Rapid Screening Tests for Determining In Vitro Susceptibility of Herpes Simplex Virus Clinical Isolates

Article Usage Stats Services JCM Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter current issue JCM About JCM Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Com...

Full description

Saved in:
Bibliographic Details
Published inJournal of Clinical Microbiology Vol. 36; no. 8; pp. 2389 - 2391
Main Authors DE LA IGLESIA, P, MELON, S, LOPEZ, B, RODRIGUEZ, M, BLANCO, M. I, MELLADO, P, DE ONA, M
Format Journal Article
LanguageEnglish
Published Washington, DC American Society for Microbiology 01.08.1998
SeriesNote
Subjects
Acyclovir - pharmacology
Antibiotics. Antiinfectious agents. Antiparasitic agents
Antifungal agents
Antiviral Agents - pharmacology
Biological and medical sciences
Cells, Cultured
Herpes Simplex - virology
Herpesvirus 1, Human - drug effects
Herpesvirus 1, Human - growth & development
Herpesvirus 2, Human - drug effects
Herpesvirus 2, Human - growth & development
Humans
Medical sciences
Microbial Sensitivity Tests
Pharmacology. Drug treatments
Virology
Virus
Performance evaluation
Acyclic nucleoside
Purine nucleoside
Drug susceptibility test
Herpesviridae
Alphaherpesvirinae
Aciclovir
Antiviral
Herpesvirus hominis
Comparative study
Online AccessGet full text

Cover

Abstract Article Usage Stats Services JCM Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter current issue JCM About JCM Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy JCM RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • journals@asmusa.org Print ISSN: 0095-1137 Online ISSN: 1098-660X Copyright © 2014 by the American Society for Microbiology.   For an alternate route to JCM .asm.org, visit: JCM       
AbstractList The susceptibility of human herpes simplex virus (HSV) to acyclovir (ACV) was determined with the use of a single dose of the drug (1 and 2 μg of ACV per ml for HSV-1 and HSV-2, respectively) in two rapid assays: a rapid cytopathic effect inhibitory assay (Rapid CIA) and a rapid dye uptake assay (Rapid DUA). These tests allow the simultaneous determination of virus titer and susceptibility to ACV at a determined viral concentration (100 50% tissue culture infective doses and 100 50% dye uptake units). These tests were compared with a conventional susceptibility assay (dye uptake assay) and showed similar results. Indeterminate results with the Rapid CIA appeared in 3 of 30 samples. With the use of both Rapid CIA and Rapid DUA, we were able to determine the susceptibility of 100% of the isolates. The rapid tests, unlike conventional assays, are able to provide susceptibility results within 3 days after the virus has been isolated from a clinical specimen and could thus play a direct role in therapeutic decisions.
The susceptibility of human herpes simplex virus (HSV) to acyclovir (ACV) was determined with the use of a single dose of the drug (1 and 2 micrograms of ACV per ml for HSV-1 and HSV-2, respectively) in two rapid assays: a rapid cytopathic effect inhibitory assay (Rapid CIA) and a rapid dye uptake assay (Rapid DUA). These tests allow the simultaneous determination of virus titer and susceptibility to ACV at a determined viral concentration (100 50% tissue culture infective doses and 100 50% dye uptake units). These tests were compared with a conventional susceptibility assay (dye uptake assay) and showed similar results. Indeterminate results with the Rapid CIA appeared in 3 of 30 samples. With the use of both Rapid CIA and Rapid DUA, we were able to determine the susceptibility of 100% of the isolates. The rapid tests, unlike conventional assays, are able to provide susceptibility results within 3 days after the virus has been isolated from a clinical specimen and could thus play a direct role in therapeutic decisions.
ABSTRACT The susceptibility of human herpes simplex virus (HSV) to acyclovir (ACV) was determined with the use of a single dose of the drug (1 and 2 μg of ACV per ml for HSV-1 and HSV-2, respectively) in two rapid assays: a rapid cytopathic effect inhibitory assay (Rapid CIA) and a rapid dye uptake assay (Rapid DUA). These tests allow the simultaneous determination of virus titer and susceptibility to ACV at a determined viral concentration (100 50% tissue culture infective doses and 100 50% dye uptake units). These tests were compared with a conventional susceptibility assay (dye uptake assay) and showed similar results. Indeterminate results with the Rapid CIA appeared in 3 of 30 samples. With the use of both Rapid CIA and Rapid DUA, we were able to determine the susceptibility of 100% of the isolates. The rapid tests, unlike conventional assays, are able to provide susceptibility results within 3 days after the virus has been isolated from a clinical specimen and could thus play a direct role in therapeutic decisions.
The susceptibility of human herpes simplex virus (HSV) to acyclovir (ACV) was determined with the use of a single dose of the drug (1 and 2 mu g of ACV per ml for HSV-1 and HSV-2, respectively) in two rapid assays: a rapid cytopathic effect inhibitory assay (Rapid CIA) and a rapid dye uptake assay (Rapid DUA). These tests allow the simultaneous determination of virus titer and susceptibility to ACV at a determined viral concentration (100 50% tissue culture infective doses and 100 50% dye uptake units). These tests were compared with a conventional susceptibility assay (dye uptake assay) and showed similar results. Indeterminate results with the Rapid CIA appeared in 3 of 30 samples. With the use of both Rapid CIA and Rapid DUA, we were able to determine the susceptibility of 100% of the isolates. The rapid tests, unlike conventional assays, are able to provide susceptibility results within 3 days after the virus has been isolated from a clinical specimen and could thus play a direct role in therapeutic decisions.
Article Usage Stats Services JCM Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter current issue JCM About JCM Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy JCM RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • journals@asmusa.org Print ISSN: 0095-1137 Online ISSN: 1098-660X Copyright © 2014 by the American Society for Microbiology.   For an alternate route to JCM .asm.org, visit: JCM       
Author Maria I. Blanco
Maria de Oña
Mercedes Rodriguez
Santiago Melón
Pedro de la Iglesia
Beatriz López
Purificación Mellado
AuthorAffiliation Laboratorio de Virología, Servicio de Microbiologia, Hospital Covadonga, 33006 Oviedo, Asturias, Spain
AuthorAffiliation_xml – name: Laboratorio de Virología, Servicio de Microbiologia, Hospital Covadonga, 33006 Oviedo, Asturias, Spain
Author_xml – sequence: 1
  givenname: P
  surname: DE LA IGLESIA
  fullname: DE LA IGLESIA, P
  organization: Laboratorio de Virología, Servicio de Microbiologia, Hospital Covadonga, 33006 Oviedo, Asturias, Spain
– sequence: 2
  givenname: S
  surname: MELON
  fullname: MELON, S
  organization: Laboratorio de Virología, Servicio de Microbiologia, Hospital Covadonga, 33006 Oviedo, Asturias, Spain
– sequence: 3
  givenname: B
  surname: LOPEZ
  fullname: LOPEZ, B
  organization: Laboratorio de Virología, Servicio de Microbiologia, Hospital Covadonga, 33006 Oviedo, Asturias, Spain
– sequence: 4
  givenname: M
  surname: RODRIGUEZ
  fullname: RODRIGUEZ, M
  organization: Laboratorio de Virología, Servicio de Microbiologia, Hospital Covadonga, 33006 Oviedo, Asturias, Spain
– sequence: 5
  givenname: M. I
  surname: BLANCO
  fullname: BLANCO, M. I
  organization: Laboratorio de Virología, Servicio de Microbiologia, Hospital Covadonga, 33006 Oviedo, Asturias, Spain
– sequence: 6
  givenname: P
  surname: MELLADO
  fullname: MELLADO, P
  organization: Laboratorio de Virología, Servicio de Microbiologia, Hospital Covadonga, 33006 Oviedo, Asturias, Spain
– sequence: 7
  givenname: M
  surname: DE ONA
  fullname: DE ONA, M
  organization: Laboratorio de Virología, Servicio de Microbiologia, Hospital Covadonga, 33006 Oviedo, Asturias, Spain
BackLink http://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2353924$$DView record in Pascal Francis
https://www.ncbi.nlm.nih.gov/pubmed/9666034$$D View this record in MEDLINE/PubMed
BookMark eNqFkkuP0zAUhS00aOgUfgLCSIhdgh0_Yi9YoPKYokFIdEDsLI9703qUxMFOgPn3OLQqsGJl6d7v2Pee4wt01oceEHpCSUlppV7cuq5kslRlxZQuKqZpSbVW99CCEq0KKcnXM7QgRIuCUlY_QBcp3RJCORfiHJ1rmQnGF2j3yQ5-izcuAvS-3-FrSGPCTYj4NYwQO_-7uu7xFz_GgDdTcjCM_sa3frzDocGXEAdIeOO7oYWfGYtTwqs265xt8TqF1o6QHqL7jW0TPDqeS_T57Zvr1WVx9fHdevXqqnBc12Ph8sC0riupa-5coyqhBYADKiiwZquo41VTa24V45RtGyEl8C3jUksQtZRsiV4e7h2mmw62Dvox2tYM0Xc23plgvfm30_u92YXvhhJBsiVL9Pyoj-HblL0wnc8bt63tIUzJqOyhVqT-L0ilqGTOJYP1AXQxpBShOQ1DiZmzNO9XHwyTRpk5SzNnaeYss_Lx37ucdMfwcv_ZsW9T9rqJtnc-nbCKCaarGXt6wPZ-t__hIxibOpM_0J9H2S-pHbZG
CODEN JCMIDW
CitedBy_id crossref_primary_10_1128_CDLI_9_6_1379_1381_2002
crossref_primary_10_1078_094471102321616463
crossref_primary_10_1007_s11274_009_0147_7
crossref_primary_10_1016_j_ijpharm_2005_11_025
crossref_primary_10_3390_ijms222011013
crossref_primary_10_1016_S0255_0857_21_02118_6
crossref_primary_10_1590_abd1806_4841_20142574
crossref_primary_10_1016_S0166_3542_99_00039_X
crossref_primary_10_1016_S0166_0934_02_00103_9
crossref_primary_10_1128_AAC_44_4_873_878_2000
Cites_doi 10.1128/AAC.31.10.1465
10.1056/NEJM199209103271108
10.1016/0166-3542(83)90001-3
10.1093/jac/12.suppl_B.137
10.1128/AAC.36.8.1589
10.7326/0003-4819-76-3-112-6-416
10.1056/NEJM199312093292405
ContentType Journal Article
Copyright 1998 INIST-CNRS
Copyright © 1998, American Society for Microbiology 1998
Copyright_xml – notice: 1998 INIST-CNRS
– notice: Copyright © 1998, American Society for Microbiology 1998
DBID IQODW
CGR
CUY
CVF
ECM
EIF
NPM
AAYXX
CITATION
7T7
7U9
8FD
C1K
FR3
H94
P64
7X8
5PM
DOI 10.1128/jcm.36.8.2389-2391.1998
DatabaseName Pascal-Francis
Medline
MEDLINE
MEDLINE (Ovid)
MEDLINE
MEDLINE
PubMed
CrossRef
Industrial and Applied Microbiology Abstracts (Microbiology A)
Virology and AIDS Abstracts
Technology Research Database
Environmental Sciences and Pollution Management
Engineering Research Database
AIDS and Cancer Research Abstracts
Biotechnology and BioEngineering Abstracts
MEDLINE - Academic
PubMed Central (Full Participant titles)
DatabaseTitle MEDLINE
Medline Complete
MEDLINE with Full Text
PubMed
MEDLINE (Ovid)
CrossRef
Virology and AIDS Abstracts
Technology Research Database
AIDS and Cancer Research Abstracts
Engineering Research Database
Industrial and Applied Microbiology Abstracts (Microbiology A)
Biotechnology and BioEngineering Abstracts
Environmental Sciences and Pollution Management
MEDLINE - Academic
DatabaseTitleList
MEDLINE
CrossRef
Virology and AIDS Abstracts
Database_xml – sequence: 1
  dbid: NPM
  name: PubMed
  url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
– sequence: 2
  dbid: EIF
  name: MEDLINE
  url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search
  sourceTypes: Index Database
DeliveryMethod fulltext_linktorsrc
Discipline Medicine
Biology
EISSN 1098-660X
EndPage 2391
ExternalDocumentID 10_1128_JCM_36_8_2389_2391_1998
9666034
2353924
jcm_36_8_2389
Genre Journal Article
GroupedDBID ---
.55
.GJ
08R
0R~
18M
29K
2WC
39C
3O-
4.4
41~
53G
5GY
5RE
5VS
AAUGY
AAYOK
ABOCM
ABPPZ
ACGFO
ADBBV
AENEX
AFMIJ
AGCDD
AI.
ALMA_UNASSIGNED_HOLDINGS
AOIJS
BAWUL
BTFSW
CS3
D-I
DIK
DU5
E3Z
EBS
EJD
F5P
FRP
GX1
H13
HF~
HYE
HZ~
H~9
IQODW
KQ8
L7B
O9-
OHT
OK1
P2P
P6G
RHF
RHI
RNS
RPM
RSF
TR2
UCJ
VH1
W8F
WHG
WOQ
X7M
ZA5
ZCA
ZGI
ZXP
~KM
AGVNZ
CGR
CUY
CVF
ECM
EIF
NPM
AAYXX
CITATION
7T7
7U9
8FD
C1K
FR3
H94
P64
7X8
5PM
ID FETCH-LOGICAL-c497t-c09517726974ccf82595eece151e3fd81c42f794a83413df566e4d34696e57663
IEDL.DBID RPM
ISSN 0095-1137
IngestDate Tue Sep 17 21:06:41 EDT 2024
Fri Oct 25 11:42:09 EDT 2024
Fri Oct 25 00:59:28 EDT 2024
Thu Sep 12 17:24:02 EDT 2024
Sat Sep 28 07:35:03 EDT 2024
Sun Oct 29 17:09:42 EDT 2023
Wed May 18 15:25:49 EDT 2016
IsDoiOpenAccess false
IsOpenAccess true
IsPeerReviewed true
IsScholarly true
Issue 8
Keywords Virus
Performance evaluation
Acyclic nucleoside
Purine nucleoside
Drug susceptibility test
Herpesviridae
Alphaherpesvirinae
Aciclovir
Antiviral
Herpesvirus hominis
Comparative study
Language English
License CC BY 4.0
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-c497t-c09517726974ccf82595eece151e3fd81c42f794a83413df566e4d34696e57663
Notes ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ObjectType-Article-1
ObjectType-Feature-2
Corresponding author. Mailing address: Laboratorio de Virología, Servicio de Microbiología, Hospital Covadonga, Celestino Villamil, s. n., 33006 Oviedo, Asturias, Spain. Phone: 34-8-5108720. Fax: 34-8-5108015. E-mail: virologia@hcq.es.
OpenAccessLink https://jcm.asm.org/content/jcm/36/8/2389.full.pdf
PMID 9666034
PQID 16526239
PQPubID 23462
PageCount 3
ParticipantIDs pubmed_primary_9666034
proquest_miscellaneous_16526239
highwire_asm_jcm_36_8_2389
crossref_primary_10_1128_JCM_36_8_2389_2391_1998
pubmedcentral_primary_oai_pubmedcentral_nih_gov_105060
proquest_miscellaneous_80019807
pascalfrancis_primary_2353924
PublicationCentury 1900
PublicationDate 1998-08-01
PublicationDateYYYYMMDD 1998-08-01
PublicationDate_xml – month: 08
  year: 1998
  text: 1998-08-01
  day: 01
PublicationDecade 1990
PublicationPlace Washington, DC
PublicationPlace_xml – name: Washington, DC
– name: United States
PublicationSeriesTitle Note
PublicationTitle Journal of Clinical Microbiology
PublicationTitleAlternate J Clin Microbiol
PublicationYear 1998
Publisher American Society for Microbiology
Publisher_xml – name: American Society for Microbiology
References 2829708 - Antimicrob Agents Chemother. 1987 Oct;31(10):1465-9
8232486 - N Engl J Med. 1993 Dec 9;329(24):1777-82
2155570 - Ann Intern Med. 1990 Mar 15;112(6):416-22
6313593 - J Antimicrob Chemother. 1983 Sep;12 Suppl B:137-52
6320716 - Antiviral Res. 1983 Nov;3(4):223-34
1288525 - N Engl J Med. 1992 Sep 10;327(11):782-9
1416838 - Antimicrob Agents Chemother. 1992 Aug;36(8):1589-95
e_1_3_2_9_2
e_1_3_2_8_2
e_1_3_2_7_2
e_1_3_2_6_2
e_1_3_2_5_2
e_1_3_2_4_2
e_1_3_2_3_2
e_1_3_2_2_2
References_xml – ident: e_1_3_2_5_2
– ident: e_1_3_2_8_2
  doi: 10.1128/AAC.31.10.1465
– ident: e_1_3_2_9_2
  doi: 10.1056/NEJM199209103271108
– ident: e_1_3_2_7_2
  doi: 10.1016/0166-3542(83)90001-3
– ident: e_1_3_2_3_2
  doi: 10.1093/jac/12.suppl_B.137
– ident: e_1_3_2_2_2
  doi: 10.1128/AAC.36.8.1589
– ident: e_1_3_2_4_2
  doi: 10.7326/0003-4819-76-3-112-6-416
– ident: e_1_3_2_6_2
  doi: 10.1056/NEJM199312093292405
SSID ssj0014455
Score 1.6526964
Snippet Article Usage Stats Services JCM Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley...
The susceptibility of human herpes simplex virus (HSV) to acyclovir (ACV) was determined with the use of a single dose of the drug (1 and 2 micrograms of ACV...
ABSTRACT The susceptibility of human herpes simplex virus (HSV) to acyclovir (ACV) was determined with the use of a single dose of the drug (1 and 2 μg of ACV...
The susceptibility of human herpes simplex virus (HSV) to acyclovir (ACV) was determined with the use of a single dose of the drug (1 and 2 mu g of ACV per ml...
The susceptibility of human herpes simplex virus (HSV) to acyclovir (ACV) was determined with the use of a single dose of the drug (1 and 2 μg of ACV per ml...
SourceID pubmedcentral
proquest
crossref
pubmed
pascalfrancis
highwire
SourceType Open Access Repository
Aggregation Database
Index Database
Publisher
StartPage 2389
SubjectTerms Acyclovir - pharmacology
Antibiotics. Antiinfectious agents. Antiparasitic agents
Antifungal agents
Antiviral Agents - pharmacology
Biological and medical sciences
Cells, Cultured
Herpes Simplex - virology
Herpesvirus 1, Human - drug effects
Herpesvirus 1, Human - growth & development
Herpesvirus 2, Human - drug effects
Herpesvirus 2, Human - growth & development
Humans
Medical sciences
Microbial Sensitivity Tests
Pharmacology. Drug treatments
Virology
Title Rapid Screening Tests for Determining In Vitro Susceptibility of Herpes Simplex Virus Clinical Isolates
URI http://jcm.asm.org/content/36/8/2389.abstract
https://www.ncbi.nlm.nih.gov/pubmed/9666034
https://search.proquest.com/docview/16526239
https://search.proquest.com/docview/80019807
https://pubmed.ncbi.nlm.nih.gov/PMC105060
Volume 36
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3Pb9MwFH6ik4Z2QTCYyGDDB65J4_hHneNUGN2kIkQ31JuVOA4LWtOqaSX23-85cQpFcOEaO7bi7znve_Ln9wDe25RxJS0LWZ7nIbcp7rlM8FBZIcwoycq8zdM9_Swnt_x6Lub-UljjZZW1yauovl9EdXXXaitXCzPsdWLDL9MxcoJYxsMBDNA--wjdnxxwLrqqBakIKWUjr-nC3_Dwh1lETEYqQjeVhglLqburp47gEDm_jBnf9019vmAnl8waXLGyK3XxNy76p6TyNx91-RyeeXJJLrqPeAFPbH0Mh125yYdjeDr1B-kv4fvXbFUVZGac7Aa9F7nBeRqCDJZ88AIZ9_SqJt-qzXpJZtum1b-0UtoHsizJxK5XtiGzyqUX_ond1tuG-Cyj9-QKTdqx2Fdwe_nxZjwJfc2F0PB0tAmNo1zIuCXGGcaUGD-mwlpjkRhYVhaKGp6UuIcz5dxfUSIbtLxgGGRLi6GLZCdwUC9r-xqIK4uEETBVjqYZznPDckkNLbIC52A8gLhfbb3qUmvoNiRJlL4eTzWTWmkHlXZQaQdVAKc9KjprFhoR_dUtgLM9nHaDJkwgC8T53vW4adxD7mAkq-1y22gqRYI0MP13D-WosIpHAZx0OO8G96YTgNwzgF27S9-934JW3abx7qz49H9ffANH3f1IJ0Z8Cweb9daeIUHa5Ocw-DSn5-2-eATVkw7u
link.rule.ids 230,315,730,783,787,888,27936,27937,53804,53806
linkProvider National Library of Medicine
linkToHtml http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3Pb9MwFH6CocEu_BhMZDDmA9ekSey4zhEVpnasE6It2s1KHAcCa1o1icT463lOnEInOMA1duIk_p7f9-TP7wG81jFlgmvq0jRNXaZjtLkkYq7QUaSGYZKnbZ7u6SUfL9j5VXRlD4VVVlZZqrTwyuulVxZfWm3leqkGvU5s8GE6Qk7gc39wF-6hufqsj9Ht3gFjUVe3II7cIKBDq-rChXjwVS09yj3hoaOK3ZDGgTmtJw5gH1k_9ynb9U59xmAjmEwq_Gd5V-ziT2z0tqjyNy919ggW_fd14pRvXlOnnvpxK_Xjv_6Ax_DQ0lbypmt9And0eQj7XSHLm0O4P7Vb9E_h88dkXWRkpoygB_0imeP7VwS5MXlrpTfm6qQkn4p6syKzpmqVNa1I94ascjLWm7WuyKwwiYu_Y7dNUxGbv_SaTNBYDD9-Bouzd_PR2LXVHFzF4mHtKkPmkMtzjGCUyjEyjSOtlUbKoWmeiUCxMMfVIRHGsWY58kzNMorhO9cYFHF6BHvlqtTPgZiCSxhbB8IQQMVYqmjKAxVkSYZjUOaA38-iXHdJO2Qb7IRCno-mknIppIGANBCQBgIOHPezLZNqKREpv7o5cLIz_9uHhjRCfonjnfZ4kGidZsslKfWqqWTAoxAJZvz3HsKQbOEPHTjq8LN9uIWkA3wHWNt2kxh8twXh0iYI7-Bx_L83nsKD8Xx6IS8ml-9fwEF3CtNIHl_CXr1p9AnSsDp91VrdT6vJL64
linkToPdf http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Nb9QwEB2Vola98FGoSKHUB67JJrHjdY7VltVuYauKbVE5WYnjlEA3G20SifbXM87HwlZw6TV2Ytl-k3kjP88AfNAhZYJratM4jm2mQ7S5KGC20EGghn6Uxk2e7tk5n1yxs-vgegtEfxemEe2rOHPy24WTZ98bbWWxUINeJza4mI2QE7jcHRRJOngCT9FkXd7H6d35AWNBW7sgDGzPo8NO2YU_48EPtXAod4SDziq0fRp65sae2IMdZP7cpWzTQ_VZg41oMipx3dK24MW_GOlDYeVfnmr8HL71c2wFKj-duooddf8g_eNjFuEFPOvoKzlpe7yELZ3vw05b0PJuH3Zn3VH9K7j5EhVZQubKCHvQP5JLnENJkCOT006CY55Oc_I1q1ZLMq_LRmHTiHXvyDIlE70qdEnmmUlg_Au7reqSdHlMb8kUjcbw5NdwNf54OZrYXVUHW7FwWNnKkDrk9BwjGaVSjFDDQGulkXpomibCU8xP8S8RCeNgkxT5pmYJxTCeawyOOD2A7XyZ6zdATOEljLE9YYigYixWNOae8pIowTEos8Dtd1IWbfIO2QQ9vpBno5mkXAppYCANDKSBgQWH_Y7LqFxIRMufbhYcbWBg_VGfBsgzcbzjHhMSrdQcvUS5Xtal9HjgI9EM_99DGLIt3KEFBy2G1h_vYGkB3wDXut0kCN9sQcg0icJbiBw-9sVj2L04HcvP0_NPb2GvvYxplI_vYLta1foI2VgVv28M7zcvozIu
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Rapid+Screening+Tests+for+Determining+In+Vitro+Susceptibility+of+Herpes+Simplex+Virus+Clinical+Isolates&rft.jtitle=Journal+of+Clinical+Microbiology&rft.au=Pedro+de+la+Iglesia&rft.au=Santiago+Mel%C3%B3n&rft.au=Beatriz+L%C3%B3pez&rft.au=Mercedes+Rodriguez&rft.date=1998-08-01&rft.pub=American+Society+for+Microbiology&rft.issn=0095-1137&rft.eissn=1098-660X&rft.volume=36&rft.issue=8&rft.spage=2389&rft_id=info:doi/10.1128%2Fjcm.36.8.2389-2391.1998&rft_id=info%3Apmid%2F9666034&rft.externalDBID=n%2Fa&rft.externalDocID=jcm_36_8_2389
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0095-1137&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0095-1137&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0095-1137&client=summon