Energy Metabolism in Mesenchymal Stem Cells During Osteogenic Differentiation
There is emerging interest in stem cell energy metabolism and its effect on differentiation. Bioenergetic changes in differentiating bone marrow mesenchymal stem cells (MSCs) are poorly understood and were the focus of our study. Using bioenergetic profiling and transcriptomics, we have established...
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| Published in | Stem cells and development Vol. 25; no. 2; p. 114 |
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| Main Authors | , , , , |
| Format | Journal Article |
| Language | English |
| Published |
United States
15.01.2016
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| Subjects | |
| Online Access | Get more information |
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| Abstract | There is emerging interest in stem cell energy metabolism and its effect on differentiation. Bioenergetic changes in differentiating bone marrow mesenchymal stem cells (MSCs) are poorly understood and were the focus of our study. Using bioenergetic profiling and transcriptomics, we have established that MSCs activate the mitochondrial process of oxidative phosphorylation (OxPhos) during osteogenic differentiation, but they maintain levels of glycolysis similar to undifferentiated cells. Consistent with their glycolytic phenotype, undifferentiated MSCs have high levels of hypoxia-inducible factor 1 (HIF-1). Osteogenically induced MSCs downregulate HIF-1 and this downregulation is required for activation of OxPhos. In summary, our work provides important insights on MSC bioenergetics and proposes a HIF-based mechanism of regulation of mitochondrial OxPhos in MSCs. |
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| AbstractList | There is emerging interest in stem cell energy metabolism and its effect on differentiation. Bioenergetic changes in differentiating bone marrow mesenchymal stem cells (MSCs) are poorly understood and were the focus of our study. Using bioenergetic profiling and transcriptomics, we have established that MSCs activate the mitochondrial process of oxidative phosphorylation (OxPhos) during osteogenic differentiation, but they maintain levels of glycolysis similar to undifferentiated cells. Consistent with their glycolytic phenotype, undifferentiated MSCs have high levels of hypoxia-inducible factor 1 (HIF-1). Osteogenically induced MSCs downregulate HIF-1 and this downregulation is required for activation of OxPhos. In summary, our work provides important insights on MSC bioenergetics and proposes a HIF-based mechanism of regulation of mitochondrial OxPhos in MSCs. |
| Author | Eliseev, Roman A Bentley, Karen L de Mesy White, Noelle S Mills, Bradley N Shum, Laura C |
| Author_xml | – sequence: 1 givenname: Laura C surname: Shum fullname: Shum, Laura C organization: 1 Center for Musculoskeletal Research, University of Rochester School of Medicine and Dentistry , Rochester, New York – sequence: 2 givenname: Noelle S surname: White fullname: White, Noelle S organization: 1 Center for Musculoskeletal Research, University of Rochester School of Medicine and Dentistry , Rochester, New York – sequence: 3 givenname: Bradley N surname: Mills fullname: Mills, Bradley N organization: 2 Department of Neurology, University of Rochester School of Medicine and Dentistry , Rochester, New York – sequence: 4 givenname: Karen L de Mesy surname: Bentley fullname: Bentley, Karen L de Mesy organization: 3 Department of Pathology, University of Rochester School of Medicine and Dentistry , Rochester, New York – sequence: 5 givenname: Roman A surname: Eliseev fullname: Eliseev, Roman A organization: 3 Department of Pathology, University of Rochester School of Medicine and Dentistry , Rochester, New York |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/26487485$$D View this record in MEDLINE/PubMed |
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| SubjectTerms | Bone Marrow Cells - cytology Cell Differentiation - physiology Cell Proliferation - physiology Cells, Cultured Energy Metabolism - physiology Humans Hypoxia-Inducible Factor 1, alpha Subunit - metabolism Mesenchymal Stem Cells - metabolism Mitochondria - metabolism Osteogenesis - physiology |
| Title | Energy Metabolism in Mesenchymal Stem Cells During Osteogenic Differentiation |
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