Successive determination of urinary bilirubin and creatinine employing simultaneous injection effective mixing flow analysis

A novel four-channel simultaneous injection effective mixing flow analysis (SIEMA) system has been assembled for successive determination of bilirubin and creatinine in urinary samples. The chemical variables and physical parameters in the flow system were optimized for the enhancement of successive...

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Published inTalanta (Oxford) Vol. 133; pp. 71 - 76
Main Authors Ponhong, Kraingkrai, Teshima, Norio, Grudpan, Kate, Vichapong, Jitlada, Motomizu, Shoji, Sakai, Tadao
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 01.02.2015
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Abstract A novel four-channel simultaneous injection effective mixing flow analysis (SIEMA) system has been assembled for successive determination of bilirubin and creatinine in urinary samples. The chemical variables and physical parameters in the flow system were optimized for the enhancement of successive analytical performances. The interferences from urine matrices on the determination of bilirubin and creatinine were eliminated to dilute urine samples. The calibration graphs with the optimum conditions were achieved to be in 0.024–5.0mgL−1 for bilirubin and 2–100mgL−1 for creatinine. The relative standard deviations (RSDs) at 3mgL−1 of bilirubin and at 50mgL−1 of creatinine for 11 runs were 1.5 and 1.0%, respectively. The limits of detections (3σ of blank) for bilirubin and creatinine were 7µgL−1 and 0.6mgL−1, respectively. The sample throughput for stepwise detection was 22h−1. The proposed method was applied to the successive determination of bilirubin and creatinine in urine samples. •We assemble a 4-channel simultaneous injection effective mixing flow analysis system.•Dye-forming reactions for urinary bilirubin and creatinine determination are performed in this system.•We conclude the newly proposed flow method is attractive for clinical routine assay.
AbstractList A novel four-channel simultaneous injection effective mixing flow analysis (SIEMA) system has been assembled for successive determination of bilirubin and creatinine in urinary samples. The chemical variables and physical parameters in the flow system were optimized for the enhancement of successive analytical performances. The interferences from urine matrices on the determination of bilirubin and creatinine were eliminated to dilute urine samples. The calibration graphs with the optimum conditions were achieved to be in 0.024-5.0 mg L(-1) for bilirubin and 2-100 mg L(-1) for creatinine. The relative standard deviations (RSDs) at 3 mg L(-1) of bilirubin and at 50 mg L(-1) of creatinine for 11 runs were 1.5 and 1.0%, respectively. The limits of detections (3σ of blank) for bilirubin and creatinine were 7 µg L(-1) and 0.6 mg L(-1), respectively. The sample throughput for stepwise detection was 22 h(-1). The proposed method was applied to the successive determination of bilirubin and creatinine in urine samples.
A novel four-channel simultaneous injection effective mixing flow analysis (SIEMA) system has been assembled for successive determination of bilirubin and creatinine in urinary samples. The chemical variables and physical parameters in the flow system were optimized for the enhancement of successive analytical performances. The interferences from urine matrices on the determination of bilirubin and creatinine were eliminated to dilute urine samples. The calibration graphs with the optimum conditions were achieved to be in 0.024-5.0 mg L super(-1) for bilirubin and 2-100 mg L super(-1) for creatinine. The relative standard deviations (RSDs) at 3 mg L super(-1) of bilirubin and at 50 mg L super(-1) of creatinine for 11 runs were 1.5 and 1.0%, respectively. The limits of detections (3 sigma of blank) for bilirubin and creatinine were 7 mu g L super(-1) and 0.6 mg L super(-1), respectively. The sample throughput for stepwise detection was 22 h super(-1). The proposed method was applied to the successive determination of bilirubin and creatinine in urine samples.
A novel four-channel simultaneous injection effective mixing flow analysis (SIEMA) system has been assembled for successive determination of bilirubin and creatinine in urinary samples. The chemical variables and physical parameters in the flow system were optimized for the enhancement of successive analytical performances. The interferences from urine matrices on the determination of bilirubin and creatinine were eliminated to dilute urine samples. The calibration graphs with the optimum conditions were achieved to be in 0.024–5.0mgL−1 for bilirubin and 2–100mgL−1 for creatinine. The relative standard deviations (RSDs) at 3mgL−1 of bilirubin and at 50mgL−1 of creatinine for 11 runs were 1.5 and 1.0%, respectively. The limits of detections (3σ of blank) for bilirubin and creatinine were 7µgL−1 and 0.6mgL−1, respectively. The sample throughput for stepwise detection was 22h−1. The proposed method was applied to the successive determination of bilirubin and creatinine in urine samples. •We assemble a 4-channel simultaneous injection effective mixing flow analysis system.•Dye-forming reactions for urinary bilirubin and creatinine determination are performed in this system.•We conclude the newly proposed flow method is attractive for clinical routine assay.
Author Teshima, Norio
Grudpan, Kate
Sakai, Tadao
Vichapong, Jitlada
Motomizu, Shoji
Ponhong, Kraingkrai
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Keywords Creatinine
Simultaneous injection effective mixing flow analysis
Bilirubin
Urinalysis
Language English
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Snippet A novel four-channel simultaneous injection effective mixing flow analysis (SIEMA) system has been assembled for successive determination of bilirubin and...
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StartPage 71
SubjectTerms Bilirubin
Bilirubin - urine
Blanks
Calibration
Creatinine
Creatinine - urine
Equipment Design
Flow Injection Analysis - instrumentation
Humans
Limit of Detection
Mathematical analysis
Optimization
Physical properties
Simultaneous injection effective mixing flow analysis
Standard deviation
Urinalysis
Urinalysis - instrumentation
Urine
Title Successive determination of urinary bilirubin and creatinine employing simultaneous injection effective mixing flow analysis
URI https://dx.doi.org/10.1016/j.talanta.2014.05.065
https://www.ncbi.nlm.nih.gov/pubmed/25435229
https://search.proquest.com/docview/1629588563
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