Successive determination of urinary bilirubin and creatinine employing simultaneous injection effective mixing flow analysis
A novel four-channel simultaneous injection effective mixing flow analysis (SIEMA) system has been assembled for successive determination of bilirubin and creatinine in urinary samples. The chemical variables and physical parameters in the flow system were optimized for the enhancement of successive...
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Published in | Talanta (Oxford) Vol. 133; pp. 71 - 76 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
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Elsevier B.V
01.02.2015
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Abstract | A novel four-channel simultaneous injection effective mixing flow analysis (SIEMA) system has been assembled for successive determination of bilirubin and creatinine in urinary samples. The chemical variables and physical parameters in the flow system were optimized for the enhancement of successive analytical performances. The interferences from urine matrices on the determination of bilirubin and creatinine were eliminated to dilute urine samples. The calibration graphs with the optimum conditions were achieved to be in 0.024–5.0mgL−1 for bilirubin and 2–100mgL−1 for creatinine. The relative standard deviations (RSDs) at 3mgL−1 of bilirubin and at 50mgL−1 of creatinine for 11 runs were 1.5 and 1.0%, respectively. The limits of detections (3σ of blank) for bilirubin and creatinine were 7µgL−1 and 0.6mgL−1, respectively. The sample throughput for stepwise detection was 22h−1. The proposed method was applied to the successive determination of bilirubin and creatinine in urine samples.
•We assemble a 4-channel simultaneous injection effective mixing flow analysis system.•Dye-forming reactions for urinary bilirubin and creatinine determination are performed in this system.•We conclude the newly proposed flow method is attractive for clinical routine assay. |
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AbstractList | A novel four-channel simultaneous injection effective mixing flow analysis (SIEMA) system has been assembled for successive determination of bilirubin and creatinine in urinary samples. The chemical variables and physical parameters in the flow system were optimized for the enhancement of successive analytical performances. The interferences from urine matrices on the determination of bilirubin and creatinine were eliminated to dilute urine samples. The calibration graphs with the optimum conditions were achieved to be in 0.024-5.0 mg L(-1) for bilirubin and 2-100 mg L(-1) for creatinine. The relative standard deviations (RSDs) at 3 mg L(-1) of bilirubin and at 50 mg L(-1) of creatinine for 11 runs were 1.5 and 1.0%, respectively. The limits of detections (3σ of blank) for bilirubin and creatinine were 7 µg L(-1) and 0.6 mg L(-1), respectively. The sample throughput for stepwise detection was 22 h(-1). The proposed method was applied to the successive determination of bilirubin and creatinine in urine samples. A novel four-channel simultaneous injection effective mixing flow analysis (SIEMA) system has been assembled for successive determination of bilirubin and creatinine in urinary samples. The chemical variables and physical parameters in the flow system were optimized for the enhancement of successive analytical performances. The interferences from urine matrices on the determination of bilirubin and creatinine were eliminated to dilute urine samples. The calibration graphs with the optimum conditions were achieved to be in 0.024-5.0 mg L super(-1) for bilirubin and 2-100 mg L super(-1) for creatinine. The relative standard deviations (RSDs) at 3 mg L super(-1) of bilirubin and at 50 mg L super(-1) of creatinine for 11 runs were 1.5 and 1.0%, respectively. The limits of detections (3 sigma of blank) for bilirubin and creatinine were 7 mu g L super(-1) and 0.6 mg L super(-1), respectively. The sample throughput for stepwise detection was 22 h super(-1). The proposed method was applied to the successive determination of bilirubin and creatinine in urine samples. A novel four-channel simultaneous injection effective mixing flow analysis (SIEMA) system has been assembled for successive determination of bilirubin and creatinine in urinary samples. The chemical variables and physical parameters in the flow system were optimized for the enhancement of successive analytical performances. The interferences from urine matrices on the determination of bilirubin and creatinine were eliminated to dilute urine samples. The calibration graphs with the optimum conditions were achieved to be in 0.024–5.0mgL−1 for bilirubin and 2–100mgL−1 for creatinine. The relative standard deviations (RSDs) at 3mgL−1 of bilirubin and at 50mgL−1 of creatinine for 11 runs were 1.5 and 1.0%, respectively. The limits of detections (3σ of blank) for bilirubin and creatinine were 7µgL−1 and 0.6mgL−1, respectively. The sample throughput for stepwise detection was 22h−1. The proposed method was applied to the successive determination of bilirubin and creatinine in urine samples. •We assemble a 4-channel simultaneous injection effective mixing flow analysis system.•Dye-forming reactions for urinary bilirubin and creatinine determination are performed in this system.•We conclude the newly proposed flow method is attractive for clinical routine assay. |
Author | Teshima, Norio Grudpan, Kate Sakai, Tadao Vichapong, Jitlada Motomizu, Shoji Ponhong, Kraingkrai |
Author_xml | – sequence: 1 givenname: Kraingkrai surname: Ponhong fullname: Ponhong, Kraingkrai organization: Department of Applied Chemistry, Aichi Institute of Technology, 1247 Yachigusa, Yakusa-cho, Toyota 470-0392, Japan – sequence: 2 givenname: Norio surname: Teshima fullname: Teshima, Norio email: teshima@aitech.ac.jp organization: Department of Applied Chemistry, Aichi Institute of Technology, 1247 Yachigusa, Yakusa-cho, Toyota 470-0392, Japan – sequence: 3 givenname: Kate surname: Grudpan fullname: Grudpan, Kate organization: Department of Chemistry, Faculty of Science and Center of Excellence for Innovation in Analytical Science and Technology, Chiang Mai University, Chiang Mai 50200, Thailand – sequence: 4 givenname: Jitlada surname: Vichapong fullname: Vichapong, Jitlada organization: Department of Applied Chemistry, Aichi Institute of Technology, 1247 Yachigusa, Yakusa-cho, Toyota 470-0392, Japan – sequence: 5 givenname: Shoji surname: Motomizu fullname: Motomizu, Shoji organization: Graduate School of Natural Science and Technology, Okayama University, 3-1-1 Tsushimanaka, Okayama 700-8530, Japan – sequence: 6 givenname: Tadao surname: Sakai fullname: Sakai, Tadao organization: Department of Applied Chemistry, Aichi Institute of Technology, 1247 Yachigusa, Yakusa-cho, Toyota 470-0392, Japan |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/25435229$$D View this record in MEDLINE/PubMed |
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Keywords | Creatinine Simultaneous injection effective mixing flow analysis Bilirubin Urinalysis |
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SubjectTerms | Bilirubin Bilirubin - urine Blanks Calibration Creatinine Creatinine - urine Equipment Design Flow Injection Analysis - instrumentation Humans Limit of Detection Mathematical analysis Optimization Physical properties Simultaneous injection effective mixing flow analysis Standard deviation Urinalysis Urinalysis - instrumentation Urine |
Title | Successive determination of urinary bilirubin and creatinine employing simultaneous injection effective mixing flow analysis |
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