Successive determination of urinary bilirubin and creatinine employing simultaneous injection effective mixing flow analysis

• Published in: Talanta (Oxford) Vol. 133; pp. 71 - 76
• Main Authors: Ponhong, Kraingkrai, Teshima, Norio, Grudpan, Kate, Vichapong, Jitlada, Motomizu, Shoji, Sakai, Tadao
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.02.2015
• Subjects: Bilirubin; Bilirubin - urine; Blanks; Calibration; Creatinine; Creatinine - urine; Equipment Design; Flow Injection Analysis - instrumentation; Humans; Limit of Detection; Mathematical analysis; Optimization; Physical properties; Simultaneous injection effective mixing flow analysis; Standard deviation; Urinalysis; Urinalysis - instrumentation; Urine; Creatinine; Simultaneous injection effective mixing flow analysis; Bilirubin; Urinalysis
• ISSN: 0039-9140; 1873-3573
• DOI: 10.1016/j.talanta.2014.05.065

A novel four-channel simultaneous injection effective mixing flow analysis (SIEMA) system has been assembled for successive determination of bilirubin and creatinine in urinary samples. The chemical variables and physical parameters in the flow system were optimized for the enhancement of successive analytical performances. The interferences from urine matrices on the determination of bilirubin and creatinine were eliminated to dilute urine samples. The calibration graphs with the optimum conditions were achieved to be in 0.024–5.0mgL−1 for bilirubin and 2–100mgL−1 for creatinine. The relative standard deviations (RSDs) at 3mgL−1 of bilirubin and at 50mgL−1 of creatinine for 11 runs were 1.5 and 1.0%, respectively. The limits of detections (3σ of blank) for bilirubin and creatinine were 7µgL−1 and 0.6mgL−1, respectively. The sample throughput for stepwise detection was 22h−1. The proposed method was applied to the successive determination of bilirubin and creatinine in urine samples. •We assemble a 4-channel simultaneous injection effective mixing flow analysis system.•Dye-forming reactions for urinary bilirubin and creatinine determination are performed in this system.•We conclude the newly proposed flow method is attractive for clinical routine assay.