Methylation pattern of IFN-γ and IL-10 genes in periodontal tissues
DNA methylation is characterized by the addition of methyl groups in cytosines within CpG islands. Unmethylated CpGs are related to transcriptionally active structure, whereas methylated CpG recruits methyl-binding proteins that promote chromatin compaction. DNA methylation can influence the express...
Saved in:
| Published in | Immunobiology (1979) Vol. 216; no. 8; pp. 936 - 941 |
|---|---|
| Main Authors | , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Netherlands
Elsevier GmbH
01.08.2011
|
| Subjects | |
| Online Access | Get full text |
| ISSN | 0171-2985 1878-3279 1878-3279 |
| DOI | 10.1016/j.imbio.2011.01.006 |
Cover
Loading…
| Abstract | DNA methylation is characterized by the addition of methyl groups in cytosines within CpG islands. Unmethylated CpGs are related to transcriptionally active structure, whereas methylated CpG recruits methyl-binding proteins that promote chromatin compaction. DNA methylation can influence the expression of cytokines and affect the development of periodontal disease.
The purpose of the present study was to evaluate the methylation status of the interferon gamma (IFN-γ) and interleukin-10 (IL-10) genes in periodontal tissues.
Methylation-specific polymerase chain reaction (MSP) and DNA sequencing analysis were used to verify the DNA methylation status of the IFN-γ and IL-10 genes, respectively, in samples from subjects without periodontitis and individuals with chronic periodontitis. Histological sections stained by hematoxylin–eosin were used for histopathological evaluation of samples.
The methylation status of the IFN-γ and IL-10 genes was similar among the groups. Most of the samples were positive for IFN-γ methylation. Only 11% of the periodontitis group showed unmethylated DNA. Considering the IL-10 gene, no unmethylated sample was observed. The profile of total or partial methylation was detected in CpGs evaluated.
The results showed evidence that methylation of IFN-γ and IL-10 genes is a usual feature on periodontal tissues. Further studies are needed to determine the functional relevance of these alterations. |
|---|---|
| AbstractList | DNA methylation is characterized by the addition of methyl groups in cytosines within CpG islands. Unmethylated CpGs are related to transcriptionally active structure, whereas methylated CpG recruits methyl-binding proteins that promote chromatin compaction. DNA methylation can influence the expression of cytokines and affect the development of periodontal disease.UNLABELLEDDNA methylation is characterized by the addition of methyl groups in cytosines within CpG islands. Unmethylated CpGs are related to transcriptionally active structure, whereas methylated CpG recruits methyl-binding proteins that promote chromatin compaction. DNA methylation can influence the expression of cytokines and affect the development of periodontal disease.The purpose of the present study was to evaluate the methylation status of the interferon gamma (IFN-γ) and interleukin-10 (IL-10) genes in periodontal tissues.OBJECTIVESThe purpose of the present study was to evaluate the methylation status of the interferon gamma (IFN-γ) and interleukin-10 (IL-10) genes in periodontal tissues.Methylation-specific polymerase chain reaction (MSP) and DNA sequencing analysis were used to verify the DNA methylation status of the IFN-γ and IL-10 genes, respectively, in samples from subjects without periodontitis and individuals with chronic periodontitis. Histological sections stained by hematoxylin-eosin were used for histopathological evaluation of samples.DESIGNMethylation-specific polymerase chain reaction (MSP) and DNA sequencing analysis were used to verify the DNA methylation status of the IFN-γ and IL-10 genes, respectively, in samples from subjects without periodontitis and individuals with chronic periodontitis. Histological sections stained by hematoxylin-eosin were used for histopathological evaluation of samples.The methylation status of the IFN-γ and IL-10 genes was similar among the groups. Most of the samples were positive for IFN-γ methylation. Only 11% of the periodontitis group showed unmethylated DNA. Considering the IL-10 gene, no unmethylated sample was observed. The profile of total or partial methylation was detected in CpGs evaluated.RESULTSThe methylation status of the IFN-γ and IL-10 genes was similar among the groups. Most of the samples were positive for IFN-γ methylation. Only 11% of the periodontitis group showed unmethylated DNA. Considering the IL-10 gene, no unmethylated sample was observed. The profile of total or partial methylation was detected in CpGs evaluated.The results showed evidence that methylation of IFN-γ and IL-10 genes is a usual feature on periodontal tissues. Further studies are needed to determine the functional relevance of these alterations.CONCLUSIONSThe results showed evidence that methylation of IFN-γ and IL-10 genes is a usual feature on periodontal tissues. Further studies are needed to determine the functional relevance of these alterations. DNA methylation is characterized by the addition of methyl groups in cytosines within CpG islands. Unmethylated CpGs are related to transcriptionally active structure, whereas methylated CpG recruits methyl-binding proteins that promote chromatin compaction. DNA methylation can influence the expression of cytokines and affect the development of periodontal disease. The purpose of the present study was to evaluate the methylation status of the interferon gamma (IFN-γ) and interleukin-10 (IL-10) genes in periodontal tissues. Methylation-specific polymerase chain reaction (MSP) and DNA sequencing analysis were used to verify the DNA methylation status of the IFN-γ and IL-10 genes, respectively, in samples from subjects without periodontitis and individuals with chronic periodontitis. Histological sections stained by hematoxylin–eosin were used for histopathological evaluation of samples. The methylation status of the IFN-γ and IL-10 genes was similar among the groups. Most of the samples were positive for IFN-γ methylation. Only 11% of the periodontitis group showed unmethylated DNA. Considering the IL-10 gene, no unmethylated sample was observed. The profile of total or partial methylation was detected in CpGs evaluated. The results showed evidence that methylation of IFN-γ and IL-10 genes is a usual feature on periodontal tissues. Further studies are needed to determine the functional relevance of these alterations. DNA methylation is characterized by the addition of methyl groups in cytosines within CpG islands. Unmethylated CpGs are related to transcriptionally active structure, whereas methylated CpG recruits methyl-binding proteins that promote chromatin compaction. DNA methylation can influence the expression of cytokines and affect the development of periodontal disease. Objectives: The purpose of the present study was to evaluate the methylation status of the interferon gamma (IFN- gamma ) and interleukin-10 (IL-10) genes in periodontal tissues. Design: Methylation-specific polymerase chain reaction (MSP) and DNA sequencing analysis were used to verify the DNA methylation status of the IFN- gamma and IL-10 genes, respectively, in samples from subjects without periodontitis and individuals with chronic periodontitis. Histological sections stained by hematoxylin-eosin were used for histopathological evaluation of samples. Results: The methylation status of the IFN- gamma and IL-10 genes was similar among the groups. Most of the samples were positive for IFN- gamma methylation. Only 11% of the periodontitis group showed unmethylated DNA. Considering the IL-10 gene, no unmethylated sample was observed. The profile of total or partial methylation was detected in CpGs evaluated. Conclusions: The results showed evidence that methylation of IFN- gamma and IL-10 genes is a usual feature on periodontal tissues. Further studies are needed to determine the functional relevance of these alterations. Abstract DNA methylation is characterized by the addition of methyl groups in cytosines within CpG islands. Unmethylated CpGs are related to transcriptionally active structure, whereas methylated CpG recruits methyl-binding proteins that promote chromatin compaction. DNA methylation can influence the expression of cytokines and affect the development of periodontal disease. Objectives The purpose of the present study was to evaluate the methylation status of the interferon gamma ( IFN-γ ) and interleukin-10 ( IL-10 ) genes in periodontal tissues. Design Methylation-specific polymerase chain reaction (MSP) and DNA sequencing analysis were used to verify the DNA methylation status of the IFN-γ and IL-10 genes, respectively, in samples from subjects without periodontitis and individuals with chronic periodontitis. Histological sections stained by hematoxylin–eosin were used for histopathological evaluation of samples. Results The methylation status of the IFN-γ and IL-10 genes was similar among the groups. Most of the samples were positive for IFN-γ methylation. Only 11% of the periodontitis group showed unmethylated DNA. Considering the IL-10 gene, no unmethylated sample was observed. The profile of total or partial methylation was detected in CpGs evaluated. Conclusions The results showed evidence that methylation of IFN-γ and IL-10 genes is a usual feature on periodontal tissues. Further studies are needed to determine the functional relevance of these alterations. |
| Author | Viana, Michelle Beatriz Diniz, Marina Gonçalves Costa, Fernando Oliveira Gomez, Ricardo Santiago Moreira, Paula Rocha da Costa, José Eustáquio Cardoso, Fabiano Pereira |
| Author_xml | – sequence: 1 givenname: Michelle Beatriz surname: Viana fullname: Viana, Michelle Beatriz organization: Laboratory of Molecular Biology, Department of Oral Surgery and Pathology, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil – sequence: 2 givenname: Fabiano Pereira surname: Cardoso fullname: Cardoso, Fabiano Pereira organization: Laboratory of Molecular Biology, Department of Oral Surgery and Pathology, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil – sequence: 3 givenname: Marina Gonçalves surname: Diniz fullname: Diniz, Marina Gonçalves organization: Laboratory of Molecular Biology, Department of Oral Surgery and Pathology, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil – sequence: 4 givenname: Fernando Oliveira surname: Costa fullname: Costa, Fernando Oliveira organization: Department of Periodontology, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil – sequence: 5 givenname: José Eustáquio surname: da Costa fullname: da Costa, José Eustáquio organization: Department of Periodontology, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil – sequence: 6 givenname: Ricardo Santiago surname: Gomez fullname: Gomez, Ricardo Santiago organization: Laboratory of Molecular Biology, Department of Oral Surgery and Pathology, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil – sequence: 7 givenname: Paula Rocha surname: Moreira fullname: Moreira, Paula Rocha email: paularocha2003@yahoo.com.br organization: Laboratory of Cell-Cell Interactions, Department of Morphology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Avenida Antônio Carlos 6627, CEP 31.270-901, Belo Horizonte, Minas Gerais, Brazil |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/21281983$$D View this record in MEDLINE/PubMed |
| BookMark | eNqFksFuEzEURS1URNPCFyCh2bGa8J7HM-MRAgmVFiIFWABry_G8AYeJndoOUr6L_-g34WnaTSWI9SRvzr227n1n7MR5R4w9R5gjYPNqPbeblfVzDohzyAPNIzZD2cqy4m13wmaALZa8k_UpO4txDYAdb-UTdsqRS-xkNWPvP1H6uR91st4VW50SBVf4oVhcfS5v_hTa9cViWSIUP8hRLGyGKFjfe5f0WCQb447iU_Z40GOkZ3f3Oft-dfnt4mO5_PJhcfFuWRrRtalsmr5uqo4MSeBkOCJVhnoBXNbDIHs5gBbSGAIjB7FCDkOFJHSnGy3qnlfn7OXBdxv8dX43qY2NhsZRO_K7qKSsQIDgzXGyFTkc4HUmX9yRu9WGerUNdqPDXt1HlIHuAJjgYww0KGPTbV4paDsqBDXVodbqtg411aEgD0zfqB5o7-3_r3pzUFHO8reloKKx5HJUNpBJqvf2iP7tA70ZrbNGj79oT3Htd8HlmhSqyBWor9OaTFuCCPm0k8Hrfxscff4vJhHMPQ |
| CitedBy_id | crossref_primary_10_1016_j_imbio_2012_12_001 crossref_primary_10_3109_00365513_2016_1149616 crossref_primary_10_1902_jop_2013_120294 crossref_primary_10_1111_prd_12000 crossref_primary_10_1111_prd_12222 crossref_primary_10_1902_jop_2012_120531 crossref_primary_10_1111_prd_12388 crossref_primary_10_1002_JPER_18_0692 crossref_primary_10_1186_s13148_021_01074_w crossref_primary_10_1007_s00784_017_2052_z crossref_primary_10_1186_s13148_020_00982_7 crossref_primary_10_1080_20548923_2021_1882122 crossref_primary_10_1111_odi_13654 crossref_primary_10_1016_j_joen_2012_12_026 crossref_primary_10_1111_jcpe_12783 crossref_primary_10_1007_s00784_015_1460_1 crossref_primary_10_1590_1807_3107bor_2021_vol35_0095 crossref_primary_10_1007_s12010_023_04417_w crossref_primary_10_3389_fimmu_2020_01105 crossref_primary_10_1007_s00005_014_0271_4 crossref_primary_10_13005_bpj_1684 crossref_primary_10_3390_cells11203211 crossref_primary_10_1111_jre_12726 crossref_primary_10_1016_j_humimm_2013_04_037 crossref_primary_10_1111_j_1600_051X_2011_01765_x crossref_primary_10_1902_jop_2014_140559 crossref_primary_10_3390_ijms21176217 crossref_primary_10_1007_s12291_018_0740_3 |
| Cites_doi | 10.1902/jop.2010.100082 10.1177/154411130301400605 10.1038/ncponc0354 10.1016/j.leukres.2005.04.012 10.1007/s10875-007-9148-1 10.1902/annals.1999.4.1.1 10.1111/j.1600-051X.2005.00821.x 10.1006/bbrc.1999.0566 10.1111/j.1600-0757.1997.tb00199.x 10.1186/1479-5876-8-110 10.1046/j.1365-2249.2001.01448.x 10.1001/archotol.130.1.39 10.1111/j.1600-0757.2006.00188.x 10.1016/S1521-6616(03)00206-7 10.1016/j.ejca.2008.02.046 10.1111/j.1600-051X.2010.01616.x 10.1902/jop.2008.080172 10.1111/j.1600-0757.1997.tb00194.x 10.1177/154405910708600212 10.1016/j.coi.2007.07.016 10.1902/jop.2008.080220 10.1016/j.molimm.2005.11.010 10.1111/j.1600-051X.2009.01446.x 10.1177/0022034509356512 10.1034/j.1600-0765.2003.02012.x 10.4049/jimmunol.165.7.3626 10.1189/jlb.1004604 |
| ContentType | Journal Article |
| Copyright | 2011 Elsevier GmbH Elsevier GmbH Copyright © 2011 Elsevier GmbH. All rights reserved. |
| Copyright_xml | – notice: 2011 Elsevier GmbH – notice: Elsevier GmbH – notice: Copyright © 2011 Elsevier GmbH. All rights reserved. |
| DBID | AAYXX CITATION CGR CUY CVF ECM EIF NPM 7X8 7T5 7TM 8FD FR3 H94 P64 RC3 |
| DOI | 10.1016/j.imbio.2011.01.006 |
| DatabaseName | CrossRef Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed MEDLINE - Academic Immunology Abstracts Nucleic Acids Abstracts Technology Research Database Engineering Research Database AIDS and Cancer Research Abstracts Biotechnology and BioEngineering Abstracts Genetics Abstracts |
| DatabaseTitle | CrossRef MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) MEDLINE - Academic Genetics Abstracts Technology Research Database Nucleic Acids Abstracts AIDS and Cancer Research Abstracts Immunology Abstracts Engineering Research Database Biotechnology and BioEngineering Abstracts |
| DatabaseTitleList | MEDLINE - Academic Genetics Abstracts MEDLINE |
| Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Biology |
| EISSN | 1878-3279 |
| EndPage | 941 |
| ExternalDocumentID | 21281983 10_1016_j_imbio_2011_01_006 S0171298511000076 1_s2_0_S0171298511000076 |
| Genre | Research Support, Non-U.S. Gov't Journal Article |
| GroupedDBID | --- --K --M -~X .1- .55 .FO .GJ .~1 0R~ 1B1 1P~ 1RT 1~. 1~5 29I 3O- 4.4 457 4G. 53G 5GY 5RE 5VS 7-5 71M 7X7 88E 88I 8AF 8AO 8FE 8FH 8FI 8FJ 8P~ 8R4 8R5 9JM AAAJQ AABNK AAEDT AAEDW AAIKJ AAKOC AALRI AAOAW AAQFI AAQXK AARKO AATTM AAXKI AAXUO AAYWO ABBQC ABFNM ABFRF ABGSF ABJNI ABMAC ABMZM ABOHT ABUDA ABUWG ABWVN ABXDB ACDAQ ACGFO ACGFS ACGOD ACIEU ACPRK ACRLP ACRPL ACVFH ADBBV ADCNI ADEZE ADKUU ADMUD ADNMO ADUVX ADVLN AEBSH AEFWE AEHWI AEIPS AEKER AENEX AEUPX AEVXI AFJKZ AFKRA AFPUW AFRHN AFTJW AFXIZ AGCQF AGEKW AGHFR AGQPQ AGRDE AGUBO AGYEJ AHHHB AHMBA AI. AIEXJ AIGII AIIUN AIKHN AITUG AJRQY AJUYK AKBMS AKRWK AKYEP ALMA_UNASSIGNED_HOLDINGS AMRAJ ANKPU ANZVX APXCP ASPBG AVWKF AXJTR AZFZN AZQEC BBNVY BENPR BES BHPHI BKOJK BLXMC BNPGV BPHCQ BVXVI CAG CCPQU CJTIS COF CS3 DU5 DWQXO EBS EFJIC EFKBS EJD EO8 EO9 EP2 EP3 F5P FDB FEDTE FGOYB FIRID FNPLU FYGXN FYUFA G-Q GBLVA GNUQQ GROUPED_DOAJ HCIFZ HMCUK HVGLF HZ~ IHE J1W KOM LK8 LUGTX M1P M2P M2Q M41 M7P MO0 M~E N9A O-L O9- O9~ OAUVE OZT P-8 P-9 P2P PC. PHGZM PHGZT PJZUB PPXIY PQGLB PQQKQ PROAC PSQYO PUEGO Q2X Q38 R2- ROL RPZ S0X SDF SDG SES SEW SPCBC SSH SSI SSU SSZ T5F T5J T5K UKHRP UNMZH VH1 X7M Y6R Z5R ZGI ZXP ~G- 3V. AACTN AFCTW AFKWA AJOXV ALIPV AMFUW RIG 0SF AAIAV ABLVK ABYKQ AHPSJ AJBFU DOVZS EFLBG LCYCR AAYXX AGRNS CITATION CGR CUY CVF ECM EIF NPM 7X8 7T5 7TM 8FD FR3 H94 P64 RC3 |
| ID | FETCH-LOGICAL-c497t-66d5639ece802ec211e3ced40285ff8d8f0a48cce0c8f4b120f31e4a9a6a45d23 |
| IEDL.DBID | AIKHN |
| ISSN | 0171-2985 1878-3279 |
| IngestDate | Fri Sep 05 12:04:24 EDT 2025 Thu Sep 04 17:14:03 EDT 2025 Thu Apr 03 07:01:20 EDT 2025 Thu Jul 17 06:02:51 EDT 2025 Thu Apr 24 23:07:06 EDT 2025 Fri Feb 23 02:24:37 EST 2024 Sun Feb 23 10:19:13 EST 2025 Tue Aug 26 17:55:31 EDT 2025 |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 8 |
| Keywords | Inflammation Cytokines Periodontitis Methylation |
| Language | English |
| License | https://www.elsevier.com/tdm/userlicense/1.0 Copyright © 2011 Elsevier GmbH. All rights reserved. |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c497t-66d5639ece802ec211e3ced40285ff8d8f0a48cce0c8f4b120f31e4a9a6a45d23 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ObjectType-Article-2 ObjectType-Feature-1 |
| PMID | 21281983 |
| PQID | 874017025 |
| PQPubID | 23479 |
| PageCount | 6 |
| ParticipantIDs | proquest_miscellaneous_883040426 proquest_miscellaneous_874017025 pubmed_primary_21281983 crossref_citationtrail_10_1016_j_imbio_2011_01_006 crossref_primary_10_1016_j_imbio_2011_01_006 elsevier_sciencedirect_doi_10_1016_j_imbio_2011_01_006 elsevier_clinicalkeyesjournals_1_s2_0_S0171298511000076 elsevier_clinicalkey_doi_10_1016_j_imbio_2011_01_006 |
| PublicationCentury | 2000 |
| PublicationDate | 2011-08-01 |
| PublicationDateYYYYMMDD | 2011-08-01 |
| PublicationDate_xml | – month: 08 year: 2011 text: 2011-08-01 day: 01 |
| PublicationDecade | 2010 |
| PublicationPlace | Netherlands |
| PublicationPlace_xml | – name: Netherlands |
| PublicationTitle | Immunobiology (1979) |
| PublicationTitleAlternate | Immunobiology |
| PublicationYear | 2011 |
| Publisher | Elsevier GmbH |
| Publisher_xml | – name: Elsevier GmbH |
| References | Offenbacher, Barros, Beck (bib0095) 2008; 79 Wilson (bib0125) 2008; 79 Adcock, Tsaprouni, Bhavsar, Ito (bib0005) 2009; 19 Ishikawa (bib0065) 2007; 43 Kwon, Kim, Lee, Oh, Choi (bib0075) 2008; 28 Madianos, Bobetsis, Kinane (bib0090) 2005; 32 Richardson (bib0110) 2003; 109 Page (bib0105) 1997; 14 Gopissetty, Ramachandram, Singal (bib0060) 2006; 43 Szalmás, Bánáti, Koroknai, László, Fehér, Salamon, Gergely, Minárovits, Kónya (bib0120) 2008; 44 Andia, de Oliveira, Casarin, Casati, Line, de Souza (bib0010) 2010; 81 Baylin (bib0025) 2005; 2 Goldenberg, Harden, Masayesva, Há, Benoit, Westra, Koch, Sidransky, Califano (bib0055) 2004; 130 Sasaki, Hou, Belani, Wang, Uchiyama, Müller, Stashenko (bib0115) 2000; 165 Zhang, Crivello, Offenbacher, Moretti, Paquette, Barros (bib0135) 2010; 37 Loo, Jin, Cheung, Wang, Chow (bib0085) 2010; 8 Kinane, Hart (bib0070) 2003; 14 Oliveira, Damm, Andia, Salmon, Nociti, Line, de Souza (bib0100) 2009; 36 Armenante, Merola, Furia, Palmiere (bib0015) 1999; 258 Armitage (bib0020) 1999; 4 Bobetsis, Barros, Lin, Weidman, Dolinoy, Jirtle, Boggess, Beck, Offenbacher (bib0030) 2007; 86 Brakensiek, Länger, Kreipe, Lehmann (bib0040) 2005; 29 Gemmel, Marshall, Seymour (bib0050) 1997; 14 Garlet, Martins, Ferreira, Milanezi, Silva (bib0045) 2003; 38 Lappin, Macleod, Kerr, Mitchell, Kinane (bib0080) 2001; 123 Bonilla-Henao, Martínez, Sobrino, Pintado (bib0035) 2005; 78 Zhang, Barros, Niculescu, Moretti, Preisser, Offenbacher (bib0130) 2010; 89 Garlet (10.1016/j.imbio.2011.01.006_bib0045) 2003; 38 Oliveira (10.1016/j.imbio.2011.01.006_bib0100) 2009; 36 Kwon (10.1016/j.imbio.2011.01.006_bib0075) 2008; 28 Adcock (10.1016/j.imbio.2011.01.006_bib0005) 2009; 19 Armenante (10.1016/j.imbio.2011.01.006_bib0015) 1999; 258 Brakensiek (10.1016/j.imbio.2011.01.006_bib0040) 2005; 29 Lappin (10.1016/j.imbio.2011.01.006_bib0080) 2001; 123 Zhang (10.1016/j.imbio.2011.01.006_bib0130) 2010; 89 Zhang (10.1016/j.imbio.2011.01.006_bib0135) 2010; 37 Goldenberg (10.1016/j.imbio.2011.01.006_bib0055) 2004; 130 Richardson (10.1016/j.imbio.2011.01.006_bib0110) 2003; 109 Gopissetty (10.1016/j.imbio.2011.01.006_bib0060) 2006; 43 Offenbacher (10.1016/j.imbio.2011.01.006_bib0095) 2008; 79 Szalmás (10.1016/j.imbio.2011.01.006_bib0120) 2008; 44 Page (10.1016/j.imbio.2011.01.006_bib0105) 1997; 14 Andia (10.1016/j.imbio.2011.01.006_bib0010) 2010; 81 Armitage (10.1016/j.imbio.2011.01.006_bib0020) 1999; 4 Loo (10.1016/j.imbio.2011.01.006_bib0085) 2010; 8 Wilson (10.1016/j.imbio.2011.01.006_bib0125) 2008; 79 Bobetsis (10.1016/j.imbio.2011.01.006_bib0030) 2007; 86 Sasaki (10.1016/j.imbio.2011.01.006_bib0115) 2000; 165 Madianos (10.1016/j.imbio.2011.01.006_bib0090) 2005; 32 Ishikawa (10.1016/j.imbio.2011.01.006_bib0065) 2007; 43 Kinane (10.1016/j.imbio.2011.01.006_bib0070) 2003; 14 Baylin (10.1016/j.imbio.2011.01.006_bib0025) 2005; 2 Gemmel (10.1016/j.imbio.2011.01.006_bib0050) 1997; 14 Bonilla-Henao (10.1016/j.imbio.2011.01.006_bib0035) 2005; 78 |
| References_xml | – volume: 36 start-page: 719 year: 2009 end-page: 725 ident: bib0100 article-title: DNA methylation status of IL-8 gene promoter in oral cells of smokers and nonsmokers with chronic periodontitis publication-title: J. Clin. Periodontol. – volume: 38 start-page: 210 year: 2003 end-page: 217 ident: bib0045 article-title: Patterns of chemokine receptors expression in different forms of human periodontal disease publication-title: J. Periodontol. Res. – volume: 81 start-page: 1336 year: 2010 end-page: 1341 ident: bib0010 article-title: DNA methylation status of the IL-8 gene promoter in aggressive periodontitis publication-title: J. Periodontol. – volume: 130 start-page: 39 year: 2004 end-page: 44 ident: bib0055 article-title: Intraoperative molecular margin analysis in head and neck cancer publication-title: Arch. Otolaryngol. Head Neck Surg. – volume: 2 start-page: 4 year: 2005 end-page: 11 ident: bib0025 article-title: DNA methylation and gene silencing in cancer publication-title: Nat. Clin. Pract. Oncol. – volume: 43 start-page: 1729 year: 2006 end-page: 1740 ident: bib0060 article-title: DNA methylation and apoptosis publication-title: Mol. Immunol. – volume: 79 start-page: 1577 year: 2008 end-page: 1584 ident: bib0095 article-title: Rethinking periodontal inflammation publication-title: J. Periodontol. – volume: 19 start-page: 694 year: 2009 end-page: 700 ident: bib0005 article-title: Epigenetic regulation of airway inflammation publication-title: Curr. Opin. Immunol. – volume: 14 start-page: 216 year: 1997 end-page: 248 ident: bib0105 article-title: Advances in the pathogenesis of periodontitis: summary of developments, clinical implications and future directions publication-title: Periodontol. 2000 – volume: 79 start-page: 1514 year: 2008 end-page: 1519 ident: bib0125 article-title: Epigenetic regulation in the inflammatory response and relevance to common diseases publication-title: J. Periodontol. – volume: 8 start-page: 110 year: 2010 ident: bib0085 article-title: Epigenetic change in E-cadherin and Cox-2 to predict chronic periodontitis publication-title: J. Trans. Med. – volume: 78 start-page: 1339 year: 2005 end-page: 1346 ident: bib0035 article-title: Different signaling pathways inhibit DNA methylation activity and up-regulated IFN-γ in human lymphocytes publication-title: J. Leukoc. Biol. – volume: 29 start-page: 1357 year: 2005 end-page: 1360 ident: bib0040 article-title: Absence of p21 (C1P1), p27 (KIP 1) and p57 (KIP2) methylation in MDS and AML publication-title: Leuk. Res. – volume: 89 start-page: 133 year: 2010 end-page: 137 ident: bib0130 article-title: Alteration of PTGS2 promoter methylation in chronic periodontitis publication-title: J. Dent. Res. – volume: 4 start-page: 1 year: 1999 end-page: 112 ident: bib0020 publication-title: International Workshop for a Classification of Periodontal Diseases and Conditions – volume: 43 start-page: 9 year: 2007 end-page: 13 ident: bib0065 article-title: Host responses in periodontal disease: a preview publication-title: Periodontol. 2000 – volume: 37 start-page: 953 year: 2010 end-page: 961 ident: bib0135 article-title: Interferon-gamma promoter hypomethylation and increased expression in chronic periodontitis publication-title: J. Clin. Periodontol. – volume: 44 start-page: 1030 year: 2008 end-page: 1038 ident: bib0120 article-title: Lineage-specifc silencing of human IL-10 expression by promoter methylation in cervical cancer cells publication-title: Eur. J. Cancer – volume: 258 start-page: 644 year: 1999 end-page: 647 ident: bib0015 article-title: Repression of the IL-6 gene is associated with hypermethylation publication-title: Biochem. Biophys. Res. Commun. – volume: 14 start-page: 112 year: 1997 end-page: 143 ident: bib0050 article-title: Cytokines and prostaglandinsin immune homeostasis and tissue destruction in periodontal disease publication-title: Periodontol. 2000 – volume: 14 start-page: 430 year: 2003 end-page: 449 ident: bib0070 article-title: Genes and gene polymorphisms associated with periodontal disease publication-title: Crit. Rev. Oral Biol. Med. – volume: 32 start-page: 57 year: 2005 end-page: 71 ident: bib0090 article-title: Generation of inflammatory stimuli: how bacteria set up inflammatory responses in the gingiva publication-title: J. Clin. Periodontol. – volume: 123 start-page: 294 year: 2001 end-page: 300 ident: bib0080 article-title: Anti-inflammtory cytokine IL-10 and T cell cytokine profile in periodotitis granulation tissue publication-title: Clin. Exp. Immunol. – volume: 28 start-page: 139 year: 2008 end-page: 146 ident: bib0075 article-title: DNA methylation and expression of IL-4 and IFN-g gene promoter genes in patients with bronchial asthma publication-title: J. Clin. Immunol. – volume: 165 start-page: 3626 year: 2000 end-page: 3630 ident: bib0115 article-title: IL-10 but not IL-4, suppresses infection-stimulated bone resorption in vivo publication-title: J. Immunol. – volume: 86 start-page: 169 year: 2007 end-page: 174 ident: bib0030 article-title: Bacterial infection promotes DNA hypermethylation publication-title: J. Dent. Res. – volume: 109 start-page: 72 year: 2003 end-page: 79 ident: bib0110 article-title: DNA methylation and auto imune disease publication-title: Clin. Immunol. – volume: 81 start-page: 1336 issue: 9 year: 2010 ident: 10.1016/j.imbio.2011.01.006_bib0010 article-title: DNA methylation status of the IL-8 gene promoter in aggressive periodontitis publication-title: J. Periodontol. doi: 10.1902/jop.2010.100082 – volume: 14 start-page: 430 year: 2003 ident: 10.1016/j.imbio.2011.01.006_bib0070 article-title: Genes and gene polymorphisms associated with periodontal disease publication-title: Crit. Rev. Oral Biol. Med. doi: 10.1177/154411130301400605 – volume: 2 start-page: 4 issue: Suppl. 1 year: 2005 ident: 10.1016/j.imbio.2011.01.006_bib0025 article-title: DNA methylation and gene silencing in cancer publication-title: Nat. Clin. Pract. Oncol. doi: 10.1038/ncponc0354 – volume: 29 start-page: 1357 year: 2005 ident: 10.1016/j.imbio.2011.01.006_bib0040 article-title: Absence of p21 (C1P1), p27 (KIP 1) and p57 (KIP2) methylation in MDS and AML publication-title: Leuk. Res. doi: 10.1016/j.leukres.2005.04.012 – volume: 28 start-page: 139 year: 2008 ident: 10.1016/j.imbio.2011.01.006_bib0075 article-title: DNA methylation and expression of IL-4 and IFN-g gene promoter genes in patients with bronchial asthma publication-title: J. Clin. Immunol. doi: 10.1007/s10875-007-9148-1 – volume: 4 start-page: 1 year: 1999 ident: 10.1016/j.imbio.2011.01.006_bib0020 publication-title: Ann. Periodontol. doi: 10.1902/annals.1999.4.1.1 – volume: 32 start-page: 57 issue: Suppl. 6 year: 2005 ident: 10.1016/j.imbio.2011.01.006_bib0090 article-title: Generation of inflammatory stimuli: how bacteria set up inflammatory responses in the gingiva publication-title: J. Clin. Periodontol. doi: 10.1111/j.1600-051X.2005.00821.x – volume: 258 start-page: 644 issue: 3 year: 1999 ident: 10.1016/j.imbio.2011.01.006_bib0015 article-title: Repression of the IL-6 gene is associated with hypermethylation publication-title: Biochem. Biophys. Res. Commun. doi: 10.1006/bbrc.1999.0566 – volume: 14 start-page: 216 issue: 1 year: 1997 ident: 10.1016/j.imbio.2011.01.006_bib0105 article-title: Advances in the pathogenesis of periodontitis: summary of developments, clinical implications and future directions publication-title: Periodontol. 2000 doi: 10.1111/j.1600-0757.1997.tb00199.x – volume: 8 start-page: 110 year: 2010 ident: 10.1016/j.imbio.2011.01.006_bib0085 article-title: Epigenetic change in E-cadherin and Cox-2 to predict chronic periodontitis publication-title: J. Trans. Med. doi: 10.1186/1479-5876-8-110 – volume: 123 start-page: 294 year: 2001 ident: 10.1016/j.imbio.2011.01.006_bib0080 article-title: Anti-inflammtory cytokine IL-10 and T cell cytokine profile in periodotitis granulation tissue publication-title: Clin. Exp. Immunol. doi: 10.1046/j.1365-2249.2001.01448.x – volume: 130 start-page: 39 year: 2004 ident: 10.1016/j.imbio.2011.01.006_bib0055 article-title: Intraoperative molecular margin analysis in head and neck cancer publication-title: Arch. Otolaryngol. Head Neck Surg. doi: 10.1001/archotol.130.1.39 – volume: 43 start-page: 9 year: 2007 ident: 10.1016/j.imbio.2011.01.006_bib0065 article-title: Host responses in periodontal disease: a preview publication-title: Periodontol. 2000 doi: 10.1111/j.1600-0757.2006.00188.x – volume: 109 start-page: 72 issue: 1 year: 2003 ident: 10.1016/j.imbio.2011.01.006_bib0110 article-title: DNA methylation and auto imune disease publication-title: Clin. Immunol. doi: 10.1016/S1521-6616(03)00206-7 – volume: 44 start-page: 1030 issue: 7 year: 2008 ident: 10.1016/j.imbio.2011.01.006_bib0120 article-title: Lineage-specifc silencing of human IL-10 expression by promoter methylation in cervical cancer cells publication-title: Eur. J. Cancer doi: 10.1016/j.ejca.2008.02.046 – volume: 37 start-page: 953 issue: 11 year: 2010 ident: 10.1016/j.imbio.2011.01.006_bib0135 article-title: Interferon-gamma promoter hypomethylation and increased expression in chronic periodontitis publication-title: J. Clin. Periodontol. doi: 10.1111/j.1600-051X.2010.01616.x – volume: 79 start-page: 1514 issue: 8 year: 2008 ident: 10.1016/j.imbio.2011.01.006_bib0125 article-title: Epigenetic regulation in the inflammatory response and relevance to common diseases publication-title: J. Periodontol. doi: 10.1902/jop.2008.080172 – volume: 14 start-page: 112 year: 1997 ident: 10.1016/j.imbio.2011.01.006_bib0050 article-title: Cytokines and prostaglandinsin immune homeostasis and tissue destruction in periodontal disease publication-title: Periodontol. 2000 doi: 10.1111/j.1600-0757.1997.tb00194.x – volume: 86 start-page: 169 year: 2007 ident: 10.1016/j.imbio.2011.01.006_bib0030 article-title: Bacterial infection promotes DNA hypermethylation publication-title: J. Dent. Res. doi: 10.1177/154405910708600212 – volume: 19 start-page: 694 year: 2009 ident: 10.1016/j.imbio.2011.01.006_bib0005 article-title: Epigenetic regulation of airway inflammation publication-title: Curr. Opin. Immunol. doi: 10.1016/j.coi.2007.07.016 – volume: 79 start-page: 1577 issue: 8 Suppl. year: 2008 ident: 10.1016/j.imbio.2011.01.006_bib0095 article-title: Rethinking periodontal inflammation publication-title: J. Periodontol. doi: 10.1902/jop.2008.080220 – volume: 43 start-page: 1729 year: 2006 ident: 10.1016/j.imbio.2011.01.006_bib0060 article-title: DNA methylation and apoptosis publication-title: Mol. Immunol. doi: 10.1016/j.molimm.2005.11.010 – volume: 36 start-page: 719 year: 2009 ident: 10.1016/j.imbio.2011.01.006_bib0100 article-title: DNA methylation status of IL-8 gene promoter in oral cells of smokers and nonsmokers with chronic periodontitis publication-title: J. Clin. Periodontol. doi: 10.1111/j.1600-051X.2009.01446.x – volume: 89 start-page: 133 issue: 2 year: 2010 ident: 10.1016/j.imbio.2011.01.006_bib0130 article-title: Alteration of PTGS2 promoter methylation in chronic periodontitis publication-title: J. Dent. Res. doi: 10.1177/0022034509356512 – volume: 38 start-page: 210 year: 2003 ident: 10.1016/j.imbio.2011.01.006_bib0045 article-title: Patterns of chemokine receptors expression in different forms of human periodontal disease publication-title: J. Periodontol. Res. doi: 10.1034/j.1600-0765.2003.02012.x – volume: 165 start-page: 3626 issue: 7 year: 2000 ident: 10.1016/j.imbio.2011.01.006_bib0115 article-title: IL-10 but not IL-4, suppresses infection-stimulated bone resorption in vivo publication-title: J. Immunol. doi: 10.4049/jimmunol.165.7.3626 – volume: 78 start-page: 1339 year: 2005 ident: 10.1016/j.imbio.2011.01.006_bib0035 article-title: Different signaling pathways inhibit DNA methylation activity and up-regulated IFN-γ in human lymphocytes publication-title: J. Leukoc. Biol. doi: 10.1189/jlb.1004604 |
| SSID | ssj0019278 |
| Score | 2.1179004 |
| Snippet | DNA methylation is characterized by the addition of methyl groups in cytosines within CpG islands. Unmethylated CpGs are related to transcriptionally active... Abstract DNA methylation is characterized by the addition of methyl groups in cytosines within CpG islands. Unmethylated CpGs are related to transcriptionally... |
| SourceID | proquest pubmed crossref elsevier |
| SourceType | Aggregation Database Index Database Enrichment Source Publisher |
| StartPage | 936 |
| SubjectTerms | Advanced Basic Science Allergy and Immunology Base Sequence Case-Control Studies Chromatin - genetics Chromatin - immunology Chromatin - metabolism Chronic Periodontitis - genetics Chronic Periodontitis - immunology Chronic Periodontitis - metabolism CpG Islands - immunology Cytokines Cytosine - metabolism DNA Methylation - immunology Humans Inflammation Interferon-gamma - genetics Interferon-gamma - metabolism Interleukin-10 - genetics Interleukin-10 - metabolism Methylation Molecular Sequence Data Periodontitis Polymerase Chain Reaction Promoter Regions, Genetic - immunology Sequence Analysis, DNA Transcription, Genetic - genetics Transcription, Genetic - immunology |
| Title | Methylation pattern of IFN-γ and IL-10 genes in periodontal tissues |
| URI | https://www.clinicalkey.com/#!/content/1-s2.0-S0171298511000076 https://www.clinicalkey.es/playcontent/1-s2.0-S0171298511000076 https://dx.doi.org/10.1016/j.imbio.2011.01.006 https://www.ncbi.nlm.nih.gov/pubmed/21281983 https://www.proquest.com/docview/874017025 https://www.proquest.com/docview/883040426 |
| Volume | 216 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3NbtQwEB61WyFxQeV_S6l84EhYx3YS51i1Xe0Cuxeo1JvlODYKguyKbA-99KV4D56JseOshEoXCSmXRDOKNRl_nsn8AbxxuUidpjSpnNSJEHWaaCayxHD0JmRVCed8vfNimc8uxfur7GoPzoZaGJ9WGbG_x_SA1vHJJEpzsm6aySff6YWV3mII8bh8Hw4YL_NsBAen8w-z5TaYULIekJE-8QxD86GQ5tV8r5pVbOWZ-iyv-w6o-wzQcBBND-FRtCDJab_Ix7Bn2yfwoJ8pefMUzhcWZd9nuJF16J7ZkpUj8-ky-fWT6LYm848Ii-SLRznSIBEqITqnviySbMJ36J7B5fTi89ksiaMSEiPKYpPkeZ2hrWGNlZRZg16d5ShBdA5l5pyspaNaSGMsNdKJKmXU8dQKXepci6xm_DmM2lVrXwIxpk5Lp6Xv5CZqXUjOGbqJxpsurLDVGNggH2ViH3E_zuKbGhLGvqogVOWFqiheNB_D2y3Tum-jsZtcDIJXQ4UoYppCmN_NVvyNzXZxX3YqVR1TVN3RnTHkW84_1O_frySDXijcmD7aolu7uu5UmHVYoEm5g0RyxFC0kcbwoleprXBYiHBKfvS_C3sFD4ff3zQ9htHmx7V9jfbTpjqB_Xe36UncJXi3uL34DWCdGMc |
| linkProvider | Elsevier |
| linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Lb9QwEB6VIgSXqry3D_CBI2Yd20m8x6pltdvu7oVW6s1yHBsFQXZFtgcu_Cn-B7-pYydZCUG3ElJO0YwSTcafv4nnAfDOZzLxhjFaeGWolGVCDZcptQKjCVUU0vtQ7zxfZJMreX6dXu_AaV8LE9IqO-xvMT2idXdn2FlzuKqq4afQ6YWPAmOI53HZA3goU5GHvL4PPzd5HshgWjhGaRrE-9ZDMcmr-lZUy66RZxJyvO7anu6in3EbGu_DXscfyUn7ik9hx9XP4FE7UfLHczibO7R8m99GVrF3Zk2WnkzHC_r7FzF1SaYzBEXyOWAcqVAIXRBD01AUSdbxKzQv4Gr88fJ0QrtBCdTKUb6mWVamyDScdYpxZzGmcwLth6GhSr1XpfLMSGWtY1Z5WSSceZE4aUYmMzItuXgJu_Wydq-BWFsmI29U6OMmS5MrITgGiTYQF567YgC8t4-2XRfxMMziq-7Txb7oaFQdjKoZXiwbwPuN0qptorFdXPaG1319KCKaRpDfrpb_S8013apsdKIbrpn-y3MGkG00_3C--x9Jer_QuCzDWYup3fKm0XHSYY6EcouIEoigyJAG8Kp1qY1xeDzfVOLgf1_sLTyeXM5nejZdXBzCk_5HOEuOYHf9_cYdI5NaF2_iSrkFLZIYYQ |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Methylation+pattern+of+IFN-%CE%B3+and+IL-10+genes+in+periodontal+tissues&rft.jtitle=Immunobiology+%281979%29&rft.au=Viana%2C+Michelle+Beatriz&rft.au=Cardoso%2C+Fabiano+Pereira&rft.au=Diniz%2C+Marina+Gon%C3%A7alves&rft.au=Costa%2C+Fernando+Oliveira&rft.date=2011-08-01&rft.issn=0171-2985&rft.volume=216&rft.issue=8&rft.spage=936&rft.epage=941&rft_id=info:doi/10.1016%2Fj.imbio.2011.01.006&rft.externalDBID=ECK1-s2.0-S0171298511000076&rft.externalDocID=1_s2_0_S0171298511000076 |
| thumbnail_m | http://utb.summon.serialssolutions.com/2.0.0/image/custom?url=https%3A%2F%2Fcdn.clinicalkey.com%2Fck-thumbnails%2F01712985%2FS0171298511X00074%2Fcov150h.gif |