Enhanced stress-tolerance of transgenic tobacco plants expressing a human dehydroascorbate reductase gene

• Published in: Journal of plant physiology Vol. 160; no. 4; pp. 347 - 353
• Main Authors: Kwon, Suk-Yoon, Choi, Sun-Mee, Ahn, Young-Ock, Lee, Haeng-Soon, Lee, Hae-Bok, Park, Yong-Mok, Kwak, Sang-Soo
• Format: Journal Article
• Language: English
• Published: Jena Elsevier GmbH 01.04.2003; Elsevier; Elsevier Science Ltd
• Subjects: Adaptation to environment and cultivation conditions; Adaptation, Physiological; Agronomy. Soil science and plant productions; ascorbic acid; Biological and medical sciences; Cold Temperature; dehydroascorbate reductase; Fundamental and applied biological sciences. Psychology; Genetics and breeding of economic plants; glutathione; Humans; Hydrogen Peroxide - pharmacology; Metabolism; Metabolism. Physicochemical requirements; methyl viologen; Nicotiana - genetics; Nicotiana - metabolism; Nicotiana - physiology; Nicotiana tabacum; oxidative stress; Oxidoreductases - genetics; Paraquat - pharmacology; Plant Leaves - drug effects; Plant physiology and development; Plants, Genetically Modified - genetics; Plants, Genetically Modified - metabolism; Plants, Genetically Modified - physiology; Sodium Chloride - administration & dosage; transgenic tobacco plant; Varietal selection. Specialized plant breeding, plant breeding aims; ascorbic acid; transgenic tobacco plant; methyl viologen; glutathione; dehydroascorbate reductase; oxidative stress; Glutathione dehydrogenase (ascorbate); Enzyme; Tolerance; Antioxidant; Gene expression; Nicotiana tabacum; Stress; Dicotyledones; Angiospermae; Spermatophyta; Transgenic plant; Oxidoreductases; Solanaceae

To analyze the physiological role of dehydroascorbate reductase (DHAR, EC 1.8.5.1) catalyzing the reduction of DHA to ascorbate in environmental stress adaptation, T 1 transgenic tobacco ( Nicotiana tabacum cv. Xanthi) plants expressing a human DHAR gene in chloroplasts were biochemically characteri...