3D super-resolution deep-tissue imaging in living mice
Stimulated emission depletion (STED) microscopy enables the three-dimensional (3D) visualization of dynamic nanoscale structures in living cells, offering unique insights into their organization. However, 3D-STED imaging deep inside biological tissue is obstructed by optical aberrations and light sc...
Saved in:
Published in | Optica Vol. 8; no. 4; pp. 442 - 450 |
---|---|
Main Authors | , , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
Optical Society of America
20.04.2021
|
Online Access | Get full text |
Cover
Loading…
Abstract | Stimulated emission depletion (STED) microscopy enables the three-dimensional (3D) visualization of dynamic nanoscale structures in living cells, offering unique insights into their organization. However, 3D-STED imaging deep inside biological tissue is obstructed by optical aberrations and light scattering. We present a STED system that overcomes these challenges. Through the combination of two-photon excitation, adaptive optics, red-emitting organic dyes, and a long-working-distance water-immersion objective lens, our system achieves aberration-corrected 3D super-resolution imaging, which we demonstrate 164 µm deep in fixed mouse brain tissue and 76 µm deep in the brain of a living mouse. |
---|---|
AbstractList | Stimulated emission depletion (STED) microscopy enables the three-dimensional (3D) visualization of dynamic nanoscale structures in living cells, offering unique insights into their organization. However, 3D-STED imaging deep inside biological tissue is obstructed by optical aberrations and light scattering. We present a STED system that overcomes these challenges. Through the combination of two-photon excitation, adaptive optics, red-emitting organic dyes, and a long-working-distance water-immersion objective lens, our system achieves aberration-corrected 3D super-resolution imaging, which we demonstrate 164 µm deep in fixed mouse brain tissue and 76 µm deep in the brain of a living mouse. |
Author | Velasco, Mary Grace M Bewersdorf, Joerg Barentine, Andrew E S Zhang, Mengyang Allgeyer, Edward S Grutzendler, Jaime Yuan, Peng Kidd, Phylicia M'Saad, Ons Antonello, Jacopo May, Dennis Booth, Martin J Greco, Valentina |
Author_xml | – sequence: 1 givenname: Mary Grace M surname: Velasco fullname: Velasco, Mary Grace M organization: Department of Cell Biology, Yale School of Medicine, New Haven, Connecticut 06520, USA – sequence: 2 givenname: Mengyang surname: Zhang fullname: Zhang, Mengyang organization: Department of Neuroscience, Yale School of Medicine, New Haven, Connecticut 06520, USA – sequence: 3 givenname: Jacopo orcidid: 0000-0001-5486-315X surname: Antonello fullname: Antonello, Jacopo organization: Department of Engineering Science, University of Oxford, Oxford OX1 3PJ, UK – sequence: 4 givenname: Peng surname: Yuan fullname: Yuan, Peng organization: Current Address: Department of Biology, Stanford University, Stanford, California 94304, USA – sequence: 5 givenname: Edward S surname: Allgeyer fullname: Allgeyer, Edward S organization: Current Address: The Gurdon Institute, University of Cambridge, Cambridge CB21QN, UK – sequence: 6 givenname: Dennis surname: May fullname: May, Dennis organization: Department of Genetics, Yale School of Medicine, New Haven, Connecticut 06520, USA – sequence: 7 givenname: Ons surname: M'Saad fullname: M'Saad, Ons organization: Department of Cell Biology, Yale School of Medicine, New Haven, Connecticut 06520, USA – sequence: 8 givenname: Phylicia surname: Kidd fullname: Kidd, Phylicia organization: Department of Cell Biology, Yale School of Medicine, New Haven, Connecticut 06520, USA – sequence: 9 givenname: Andrew E S surname: Barentine fullname: Barentine, Andrew E S organization: Department of Cell Biology, Yale School of Medicine, New Haven, Connecticut 06520, USA – sequence: 10 givenname: Valentina surname: Greco fullname: Greco, Valentina organization: Department of Dermatology, Yale Stem Cell Center, Yale Cancer Center, Yale School of Medicine, New Haven, Connecticut 06520, USA – sequence: 11 givenname: Jaime surname: Grutzendler fullname: Grutzendler, Jaime organization: Department of Neurology, Yale School of Medicine, New Haven, Connecticut 06520, USA – sequence: 12 givenname: Martin J orcidid: 0000-0002-9525-8981 surname: Booth fullname: Booth, Martin J organization: Department of Engineering Science, University of Oxford, Oxford OX1 3PJ, UK – sequence: 13 givenname: Joerg surname: Bewersdorf fullname: Bewersdorf, Joerg organization: Department of Cell Biology, Yale School of Medicine, New Haven, Connecticut 06520, USA |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/34239948$$D View this record in MEDLINE/PubMed |
BookMark | eNpVkM1Lw0AQxRep2Fp79Co5eknd72QvgtRPKNRDPS-bzaSuJNmYTQr-96a0lnqaB_PjzZt3iUa1rwGha4LnhEl-55vOWTPnRKacnKEJZYzHVDA5OtFjNAvhC2NMGMdC4Qs0ZpwypXg6QZI9RqFvoI1bCL7sO-frKAdo4s6F0EPkKrNx9SZydVS67U5VzsIVOi9MGWB2mFP08fy0XrzGy9XL2-JhGVuuki7m1BJgSuRUUJKnOMPEKiPSTOU0JQnNqYUss0lRMJGbJCugUEoQmStlpOIJm6L7vW_TZxXkFuquNaVu2iFW-6O9cfr_pnafeuO3OqWciWRncHswaP13D6HTlQsWytLU4PugqRCYSpkqNqDxHrWtD6GF4niGYL2rW6_e18Nzel_3wN-cZjvSf-WyXy17fcc |
CitedBy_id | crossref_primary_10_1364_OE_434260 crossref_primary_10_1063_5_0075012 crossref_primary_10_1364_BOE_510512 crossref_primary_10_3788_CJL231342 crossref_primary_10_1088_1361_6463_abff7b crossref_primary_10_1109_TMI_2024_3358191 crossref_primary_10_1364_AO_416585 crossref_primary_10_1109_JLT_2024_3355177 crossref_primary_10_3389_fchem_2021_746900 crossref_primary_10_1002_glia_24103 crossref_primary_10_1016_j_isci_2022_104961 crossref_primary_10_1038_s41592_024_02236_3 crossref_primary_10_1073_pnas_2201861119 crossref_primary_10_1038_s42005_024_01653_2 crossref_primary_10_1364_BOE_444525 crossref_primary_10_1016_j_neures_2021_11_011 crossref_primary_10_1364_BOE_504764 crossref_primary_10_1371_journal_pone_0290550 crossref_primary_10_1117_1_APN_2_1_016009 crossref_primary_10_1017_S155192952200089X crossref_primary_10_3788_IRLA20240011 crossref_primary_10_1523_JNEUROSCI_1125_22_2022 crossref_primary_10_3389_fimmu_2023_1176594 crossref_primary_10_1063_5_0102527 crossref_primary_10_1088_1361_6501_ace731 crossref_primary_10_1364_BOE_524013 crossref_primary_10_3788_IRLA20220546 crossref_primary_10_1016_j_nbd_2021_105420 crossref_primary_10_1038_s41467_022_28904_6 crossref_primary_10_1016_j_molcel_2021_12_022 crossref_primary_10_1038_s41592_023_01936_6 crossref_primary_10_1063_5_0066331 crossref_primary_10_1364_AO_455628 crossref_primary_10_1021_acsphotonics_2c00957 crossref_primary_10_1038_s41598_022_13377_w crossref_primary_10_1088_1402_4896_acdf8e crossref_primary_10_1117_1_NPh_11_1_014415 |
Cites_doi | 10.1016/j.bpj.2011.07.027 10.1038/nrm.2017.71 10.1016/j.cell.2015.06.067 10.1103/PhysRevLett.88.163901 10.1038/nchem.1546 10.1364/OE.18.001657 10.1073/pnas.97.15.8206 10.1073/pnas.1807104115 10.1002/jbio.201300041 10.1016/j.bpj.2018.07.028 10.1007/978-1-4939-7265-4_5 10.1364/OE.27.023378 10.1083/jcb.201809107 10.1364/OPTICA.1.000181 10.1111/j.1365-2818.2005.01431.x 10.1088/1361-6463/abac81 10.1038/nmeth.4403 10.1016/j.bpj.2011.05.020 10.1364/OPEX.12.003605 10.1364/OE.24.008862 10.1038/ncomms10778 10.1364/OE.20.020998 10.1038/s41592-018-0145-5 10.1364/JOSA.66.000207 10.1016/j.bpj.2012.12.053 10.1021/nl901398t 10.1021/cb800025k 10.1534/genetics.118.300227 10.1038/nmeth818 10.1038/lsa.2014.46 10.1038/nmeth.4337 10.1364/BOE.10.001999 10.1364/OL.24.000954 10.1038/nmeth.1214 10.1021/acsphotonics.0c00388 10.1111/j.1365-2818.2004.01267.x 10.1038/nmeth.2925 10.1038/ncomms8276 10.1364/OL.19.000780 10.1073/pnas.1819965116 10.1073/pnas.1119129109 10.1364/OPEX.12.006540 10.1038/nbt899 10.1364/BOE.9.003624 10.1126/science.1215369 10.1038/srep15348 10.1007/BF02956173 10.1016/j.cell.2018.02.007 10.1364/OL.40.004915 10.1364/BOE.2.003135 10.1364/OE.17.014567 10.1364/BOE.8.003891 10.1016/j.ymeth.2019.05.020 10.1063/1.5020249 10.1021/acsnano.9b05891 |
ContentType | Journal Article |
Copyright | Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI. Published by The Optical Society under the terms of the . Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI. 2021 |
Copyright_xml | – notice: Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI. – notice: Published by The Optical Society under the terms of the . Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI. 2021 |
DBID | NPM AAYXX CITATION 7X8 5PM |
DOI | 10.1364/optica.416841 |
DatabaseName | PubMed CrossRef MEDLINE - Academic PubMed Central (Full Participant titles) |
DatabaseTitle | PubMed CrossRef MEDLINE - Academic |
DatabaseTitleList | CrossRef PubMed |
Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Physics |
EISSN | 2334-2536 |
EndPage | 450 |
ExternalDocumentID | 10_1364_OPTICA_416841 34239948 |
Genre | Journal Article |
GrantInformation_xml | – fundername: NIDDK NIH HHS grantid: P30 DK045735 – fundername: Wellcome Trust grantid: 203285/Z/16/Z – fundername: National Institutes of Health grantid: 1R01NS089734; P30-DK45735; U01 DA047734 – fundername: Wellcome Trust grantid: 203285/B/16/Z; 203285/C/16/Z – fundername: Yale’s Integrated Graduate Program in Physical and Engineering Biology – fundername: European Research Council grantid: AdOMIS 695140 – fundername: G. Harold and Leila Y. Mathers Foundation |
GroupedDBID | 4.4 AAWJZ ADBBV AENEX AKGWG ALMA_UNASSIGNED_HOLDINGS AZSQR BCNDV DSZJF EBS GROUPED_DOAJ M~E NPM OFLFD OK1 OPJBK ROL ROP ROS TR6 AAYXX CITATION 7X8 5PM |
ID | FETCH-LOGICAL-c497t-42c1e395d2521d80b01c9a58b9d28172d2cebbc7ff35da7bfef99516d99a69473 |
ISSN | 2334-2536 |
IngestDate | Tue Apr 09 21:53:18 EDT 2024 Fri Apr 12 06:19:32 EDT 2024 Wed Aug 07 14:11:41 EDT 2024 Thu May 23 23:32:00 EDT 2024 |
IsDoiOpenAccess | false |
IsOpenAccess | true |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 4 |
Language | English |
License | Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI. https://creativecommons.org/licenses/by/4.0 2334-2536/21/040442-09 |
LinkModel | OpenURL |
MergedId | FETCHMERGED-LOGICAL-c497t-42c1e395d2521d80b01c9a58b9d28172d2cebbc7ff35da7bfef99516d99a69473 |
Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ORCID | 0000-0001-5486-315X 0000-0002-9525-8981 |
OpenAccessLink | http://dx.doi.org/10.1364/OPTICA.416841 |
PMID | 34239948 |
PQID | 2550266893 |
PQPubID | 23479 |
PageCount | 9 |
ParticipantIDs | pubmedcentral_primary_oai_pubmedcentral_nih_gov_8243577 proquest_miscellaneous_2550266893 crossref_primary_10_1364_OPTICA_416841 pubmed_primary_34239948 |
PublicationCentury | 2000 |
PublicationDate | 2021-04-20 |
PublicationDateYYYYMMDD | 2021-04-20 |
PublicationDate_xml | – month: 04 year: 2021 text: 2021-04-20 day: 20 |
PublicationDecade | 2020 |
PublicationPlace | United States |
PublicationPlace_xml | – name: United States |
PublicationTitle | Optica |
PublicationTitleAlternate | Optica |
PublicationYear | 2021 |
Publisher | Optical Society of America |
Publisher_xml | – name: Optical Society of America |
References | Klar (optica-8-4-442-R11) 1999; 24 Abbe (optica-8-4-442-R2) 1873; 9 Turcotte (optica-8-4-442-R42) 2019; 116 Zheng (optica-8-4-442-R41) 2017; 14 Moneron (optica-8-4-442-R25) 2009; 17 Kilian (optica-8-4-442-R53) 2018; 15 Helmchen (optica-8-4-442-R23) 2005; 2 Winter (optica-8-4-442-R24) 2014; 1 Wang (optica-8-4-442-R40) 2014; 11 Schwertner (optica-8-4-442-R31) 2005; 217 Zdankowski (optica-8-4-442-R38) 2020; 14 Lukinavicius (optica-8-4-442-R51) 2013; 5 Barbotin (optica-8-4-442-R20) 2020; 7 Schroeder (optica-8-4-442-R3) 2018; 218 Lenz (optica-8-4-442-R17) 2014; 7 Aviles-Espinosa (optica-8-4-442-R39) 2011; 2 Tonnesen (optica-8-4-442-R55) 2018; 172 Gould (optica-8-4-442-R12) 2012; 20 Galiñanes (optica-8-4-442-R50) 2018; 9 Bianchini (optica-8-4-442-R28) 2012; 109 Masch (optica-8-4-442-R48) 2018; 115 Velasco (optica-8-4-442-R29) 2015; 40 Takasaki (optica-8-4-442-R27) 2013; 104 Hell (optica-8-4-442-R5) 1994; 19 Heine (optica-8-4-442-R22) 2018; 89 Bottanelli (optica-8-4-442-R44) 2016; 7 Ter Veer (optica-8-4-442-R26) 2017; 1663 Booth (optica-8-4-442-R33) 2014; 3 Zdankowski (optica-8-4-442-R37) 2019; 10 Zipfel (optica-8-4-442-R46) 2003; 21 Rankin (optica-8-4-442-R7) 2011; 100 Los (optica-8-4-442-R49) 2008; 3 Barbotin (optica-8-4-442-R19) 2019; 27 Klar (optica-8-4-442-R16) 2000; 97 Grimm (optica-8-4-442-R52) 2017; 14 Török (optica-8-4-442-R10) 2004; 12 Stockhammer (optica-8-4-442-R4) 2020; 54 Deng (optica-8-4-442-R18) 2010; 18 Berning (optica-8-4-442-R9) 2012; 335 Sahl (optica-8-4-442-R1) 2017; 18 Schwertner (optica-8-4-442-R34) 2004; 12 Richardson (optica-8-4-442-R30) 2015; 162 Noll (optica-8-4-442-R47) 1976; 66 Waldchen (optica-8-4-442-R54) 2015; 5 Schmidt (optica-8-4-442-R14) 2009; 9 Urban (optica-8-4-442-R21) 2011; 101 Patton (optica-8-4-442-R36) 2016; 24 Dunst (optica-8-4-442-R6) 2019; 211 Wang (optica-8-4-442-R43) 2015; 6 Turcotte (optica-8-4-442-R32) 2017; 8 Steffens (optica-8-4-442-R8) 2019; 174 Schmidt (optica-8-4-442-R15) 2008; 5 Schwertner (optica-8-4-442-R35) 2004; 213 Barentine (optica-8-4-442-R45) 2018; 115 Dyba (optica-8-4-442-R13) 2002; 88 |
References_xml | – volume: 101 start-page: 1277 year: 2011 ident: optica-8-4-442-R21 publication-title: Biophys. J. doi: 10.1016/j.bpj.2011.07.027 contributor: fullname: Urban – volume: 18 start-page: 685 year: 2017 ident: optica-8-4-442-R1 publication-title: Nat. Rev. Mol. Cell Biol. doi: 10.1038/nrm.2017.71 contributor: fullname: Sahl – volume: 162 start-page: 246 year: 2015 ident: optica-8-4-442-R30 publication-title: Cell doi: 10.1016/j.cell.2015.06.067 contributor: fullname: Richardson – volume: 88 start-page: 163901 year: 2002 ident: optica-8-4-442-R13 publication-title: Phys. Rev. Lett. doi: 10.1103/PhysRevLett.88.163901 contributor: fullname: Dyba – volume: 5 start-page: 132 year: 2013 ident: optica-8-4-442-R51 publication-title: Nature Chem. doi: 10.1038/nchem.1546 contributor: fullname: Lukinavicius – volume: 18 start-page: 1657 year: 2010 ident: optica-8-4-442-R18 publication-title: Opt. Express doi: 10.1364/OE.18.001657 contributor: fullname: Deng – volume: 97 start-page: 8206 year: 2000 ident: optica-8-4-442-R16 publication-title: Proc. Natl. Acad. Sci. USA doi: 10.1073/pnas.97.15.8206 contributor: fullname: Klar – volume: 115 start-page: E8047 year: 2018 ident: optica-8-4-442-R48 publication-title: Proc. Natl. Acad. Sci. U.S.A. doi: 10.1073/pnas.1807104115 contributor: fullname: Masch – volume: 7 start-page: 29 year: 2014 ident: optica-8-4-442-R17 publication-title: J. Biophoton. doi: 10.1002/jbio.201300041 contributor: fullname: Lenz – volume: 115 start-page: 951 year: 2018 ident: optica-8-4-442-R45 publication-title: Biophys. J. doi: 10.1016/j.bpj.2018.07.028 contributor: fullname: Barentine – volume: 1663 start-page: 45 year: 2017 ident: optica-8-4-442-R26 publication-title: Methods Mol. Biol. doi: 10.1007/978-1-4939-7265-4_5 contributor: fullname: Ter Veer – volume: 27 start-page: 23378 year: 2019 ident: optica-8-4-442-R19 publication-title: Opt. Express doi: 10.1364/OE.27.023378 contributor: fullname: Barbotin – volume: 218 start-page: 83 year: 2018 ident: optica-8-4-442-R3 publication-title: J. Cell Biol. doi: 10.1083/jcb.201809107 contributor: fullname: Schroeder – volume: 1 start-page: 181 year: 2014 ident: optica-8-4-442-R24 publication-title: Optica doi: 10.1364/OPTICA.1.000181 contributor: fullname: Winter – volume: 217 start-page: 184 year: 2005 ident: optica-8-4-442-R31 publication-title: J. Microsc. doi: 10.1111/j.1365-2818.2005.01431.x contributor: fullname: Schwertner – volume: 54 start-page: 033001 year: 2020 ident: optica-8-4-442-R4 publication-title: J. Phys. D doi: 10.1088/1361-6463/abac81 contributor: fullname: Stockhammer – volume: 14 start-page: 987 year: 2017 ident: optica-8-4-442-R52 publication-title: Nat. Methods doi: 10.1038/nmeth.4403 contributor: fullname: Grimm – volume: 100 start-page: L63 year: 2011 ident: optica-8-4-442-R7 publication-title: Biophys. J. doi: 10.1016/j.bpj.2011.05.020 contributor: fullname: Rankin – volume: 12 start-page: 3605 year: 2004 ident: optica-8-4-442-R10 publication-title: Opt. Express doi: 10.1364/OPEX.12.003605 contributor: fullname: Török – volume: 24 start-page: 8862 year: 2016 ident: optica-8-4-442-R36 publication-title: Opt. Express doi: 10.1364/OE.24.008862 contributor: fullname: Patton – volume: 7 start-page: 10778 year: 2016 ident: optica-8-4-442-R44 publication-title: Nat. Commun. doi: 10.1038/ncomms10778 contributor: fullname: Bottanelli – volume: 20 start-page: 20998 year: 2012 ident: optica-8-4-442-R12 publication-title: Opt. Express doi: 10.1364/OE.20.020998 contributor: fullname: Gould – volume: 15 start-page: 755 year: 2018 ident: optica-8-4-442-R53 publication-title: Nat. Methods doi: 10.1038/s41592-018-0145-5 contributor: fullname: Kilian – volume: 66 start-page: 207 year: 1976 ident: optica-8-4-442-R47 publication-title: J. Opt. Soc. Am. doi: 10.1364/JOSA.66.000207 contributor: fullname: Noll – volume: 104 start-page: 770 year: 2013 ident: optica-8-4-442-R27 publication-title: Biophys. J. doi: 10.1016/j.bpj.2012.12.053 contributor: fullname: Takasaki – volume: 9 start-page: 2508 year: 2009 ident: optica-8-4-442-R14 publication-title: Nano Lett. doi: 10.1021/nl901398t contributor: fullname: Schmidt – volume: 3 start-page: 373 year: 2008 ident: optica-8-4-442-R49 publication-title: ACS Chem. Biol. doi: 10.1021/cb800025k contributor: fullname: Los – volume: 211 start-page: 15 year: 2019 ident: optica-8-4-442-R6 publication-title: Genetics doi: 10.1534/genetics.118.300227 contributor: fullname: Dunst – volume: 2 start-page: 932 year: 2005 ident: optica-8-4-442-R23 publication-title: Nat. Methods doi: 10.1038/nmeth818 contributor: fullname: Helmchen – volume: 3 start-page: e165 year: 2014 ident: optica-8-4-442-R33 publication-title: Light Sci. Appl. doi: 10.1038/lsa.2014.46 contributor: fullname: Booth – volume: 14 start-page: 869 year: 2017 ident: optica-8-4-442-R41 publication-title: Nat. Methods doi: 10.1038/nmeth.4337 contributor: fullname: Zheng – volume: 10 start-page: 1999 year: 2019 ident: optica-8-4-442-R37 publication-title: Biomed. Opt. Express doi: 10.1364/BOE.10.001999 contributor: fullname: Zdankowski – volume: 24 start-page: 954 year: 1999 ident: optica-8-4-442-R11 publication-title: Opt. Lett. doi: 10.1364/OL.24.000954 contributor: fullname: Klar – volume: 5 start-page: 539 year: 2008 ident: optica-8-4-442-R15 publication-title: Nat. Methods doi: 10.1038/nmeth.1214 contributor: fullname: Schmidt – volume: 7 start-page: 1742 year: 2020 ident: optica-8-4-442-R20 publication-title: ACS Photon. doi: 10.1021/acsphotonics.0c00388 contributor: fullname: Barbotin – volume: 213 start-page: 11 year: 2004 ident: optica-8-4-442-R35 publication-title: J. Microsc. doi: 10.1111/j.1365-2818.2004.01267.x contributor: fullname: Schwertner – volume: 11 start-page: 625 year: 2014 ident: optica-8-4-442-R40 publication-title: Nat. Methods doi: 10.1038/nmeth.2925 contributor: fullname: Wang – volume: 6 start-page: 7276 year: 2015 ident: optica-8-4-442-R43 publication-title: Nat. Commun. doi: 10.1038/ncomms8276 contributor: fullname: Wang – volume: 19 start-page: 780 year: 1994 ident: optica-8-4-442-R5 publication-title: Opt. Lett. doi: 10.1364/OL.19.000780 contributor: fullname: Hell – volume: 116 start-page: 9586 year: 2019 ident: optica-8-4-442-R42 publication-title: Proc. Natl. Acad. Sci. USA doi: 10.1073/pnas.1819965116 contributor: fullname: Turcotte – volume: 109 start-page: 6390 year: 2012 ident: optica-8-4-442-R28 publication-title: Proc. Natl. Acad. Sci. USA doi: 10.1073/pnas.1119129109 contributor: fullname: Bianchini – volume: 12 start-page: 6540 year: 2004 ident: optica-8-4-442-R34 publication-title: Opt. Express doi: 10.1364/OPEX.12.006540 contributor: fullname: Schwertner – volume: 21 start-page: 1369 year: 2003 ident: optica-8-4-442-R46 publication-title: Nat. Biotechnol. doi: 10.1038/nbt899 contributor: fullname: Zipfel – volume: 9 start-page: 3624 year: 2018 ident: optica-8-4-442-R50 publication-title: Biomed. Opt. Express doi: 10.1364/BOE.9.003624 contributor: fullname: Galiñanes – volume: 335 start-page: 551 year: 2012 ident: optica-8-4-442-R9 publication-title: Science doi: 10.1126/science.1215369 contributor: fullname: Berning – volume: 5 start-page: 15348 year: 2015 ident: optica-8-4-442-R54 publication-title: Sci. Rep. doi: 10.1038/srep15348 contributor: fullname: Waldchen – volume: 9 start-page: 413 year: 1873 ident: optica-8-4-442-R2 publication-title: Archiv für mikroskopische Anatomie doi: 10.1007/BF02956173 contributor: fullname: Abbe – volume: 172 start-page: 1108 year: 2018 ident: optica-8-4-442-R55 publication-title: Cell doi: 10.1016/j.cell.2018.02.007 contributor: fullname: Tonnesen – volume: 40 start-page: 4915 year: 2015 ident: optica-8-4-442-R29 publication-title: Opt. Lett. doi: 10.1364/OL.40.004915 contributor: fullname: Velasco – volume: 2 start-page: 3135 year: 2011 ident: optica-8-4-442-R39 publication-title: Biomed. Opt. Express doi: 10.1364/BOE.2.003135 contributor: fullname: Aviles-Espinosa – volume: 17 start-page: 14567 year: 2009 ident: optica-8-4-442-R25 publication-title: Opt. Express doi: 10.1364/OE.17.014567 contributor: fullname: Moneron – volume: 8 start-page: 3891 year: 2017 ident: optica-8-4-442-R32 publication-title: Biomed. Opt. Express doi: 10.1364/BOE.8.003891 contributor: fullname: Turcotte – volume: 174 start-page: 42 year: 2019 ident: optica-8-4-442-R8 publication-title: Methods doi: 10.1016/j.ymeth.2019.05.020 contributor: fullname: Steffens – volume: 89 start-page: 053701 year: 2018 ident: optica-8-4-442-R22 publication-title: Rev. Sci. Instrum. doi: 10.1063/1.5020249 contributor: fullname: Heine – volume: 14 start-page: 394 year: 2020 ident: optica-8-4-442-R38 publication-title: ACS Nano doi: 10.1021/acsnano.9b05891 contributor: fullname: Zdankowski |
SSID | ssj0001340590 |
Score | 2.4748075 |
Snippet | Stimulated emission depletion (STED) microscopy enables the three-dimensional (3D) visualization of dynamic nanoscale structures in living cells, offering... |
SourceID | pubmedcentral proquest crossref pubmed |
SourceType | Open Access Repository Aggregation Database Index Database |
StartPage | 442 |
Title | 3D super-resolution deep-tissue imaging in living mice |
URI | https://www.ncbi.nlm.nih.gov/pubmed/34239948 https://search.proquest.com/docview/2550266893 https://pubmed.ncbi.nlm.nih.gov/PMC8243577 |
Volume | 8 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3db9MwELdgCIkXxDflS0FCvFQpqe3Y8eO0AQMJtocO7S1y_KFFYmnUpg_bX7-z46ZJ24fBS1S5iWvdXS-_O9_9jNAnk9hUcQlhCUu522ZMYqmnLGaJLTQ1Elvu-p1__WYn5_TnRXqxOW3Ud5c0xUTd7O0r-R-twhjo1XXJ_oNmu0lhAD6DfuEKGobrnXRMjsfLVW0WMcTM4YfG2pg6brw4x-VVewZRWY3_lj5zcBVK3dZ49LRuesU6fwxgaTUPPTzX4-8L6f72k53ssquFvZbhpeeTCI7Te72NA062nnfuZNWmWM9MuD2kGPDU7ZbgZOOJMCEwkJLAWb1nLLjSrGcxtOcWacugteOuCaMg49Oz2Y-jwwlAw6wlwRrSYm-9rroiQr8Xx2jePp63j99HDzAXadoLrn22jVDXZetPGgzrDoSrMMOXwQKGAGUn6tgunu2hkdkT9DiEEdFhaxNP0T1TPUMPfTmvWj5HjBxH25YR9SwjCpYRlVXUWkbkLOMFOv_2dXZ0EocTMmJFBW9iitXUEJFqDChMZy6prYRMs0JonAE01ViZolDcWpJqyQtrrABIzbQQkgnKyUt0UIF9vEaRoZYLRw1ECYDMREkFUM5myhKWZFokI_R5LZa8bolQ8r0aGKGPa6Hl4Krc_pOszHy1zCF6hYifAUIeoVetELupPBGloNkI8YF4uxscDfrwm6q89HToGQbIz_mbuy7wLXq0MfJ36KBZrMx7QJZN8cEbzS3wEXat |
link.rule.ids | 230,315,786,790,870,891,27955,27956 |
linkProvider | Directory of Open Access Journals |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=3D+super-resolution+deep-tissue+imaging+in+living+mice&rft.jtitle=Optica&rft.au=Velasco%2C+Mary+Grace+M.&rft.au=Zhang%2C+Mengyang&rft.au=Antonello%2C+Jacopo&rft.au=Yuan%2C+Peng&rft.date=2021-04-20&rft.issn=2334-2536&rft.eissn=2334-2536&rft.volume=8&rft.issue=4&rft.spage=442&rft_id=info:doi/10.1364%2FOPTICA.416841&rft.externalDBID=n%2Fa&rft.externalDocID=10_1364_OPTICA_416841 |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=2334-2536&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=2334-2536&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=2334-2536&client=summon |