3D super-resolution deep-tissue imaging in living mice

Stimulated emission depletion (STED) microscopy enables the three-dimensional (3D) visualization of dynamic nanoscale structures in living cells, offering unique insights into their organization. However, 3D-STED imaging deep inside biological tissue is obstructed by optical aberrations and light sc...

Full description

Saved in:
Bibliographic Details
Published inOptica Vol. 8; no. 4; pp. 442 - 450
Main Authors Velasco, Mary Grace M, Zhang, Mengyang, Antonello, Jacopo, Yuan, Peng, Allgeyer, Edward S, May, Dennis, M'Saad, Ons, Kidd, Phylicia, Barentine, Andrew E S, Greco, Valentina, Grutzendler, Jaime, Booth, Martin J, Bewersdorf, Joerg
Format Journal Article
LanguageEnglish
Published United States Optical Society of America 20.04.2021
Online AccessGet full text

Cover

Abstract Stimulated emission depletion (STED) microscopy enables the three-dimensional (3D) visualization of dynamic nanoscale structures in living cells, offering unique insights into their organization. However, 3D-STED imaging deep inside biological tissue is obstructed by optical aberrations and light scattering. We present a STED system that overcomes these challenges. Through the combination of two-photon excitation, adaptive optics, red-emitting organic dyes, and a long-working-distance water-immersion objective lens, our system achieves aberration-corrected 3D super-resolution imaging, which we demonstrate 164 µm deep in fixed mouse brain tissue and 76 µm deep in the brain of a living mouse.
AbstractList Stimulated emission depletion (STED) microscopy enables the three-dimensional (3D) visualization of dynamic nanoscale structures in living cells, offering unique insights into their organization. However, 3D-STED imaging deep inside biological tissue is obstructed by optical aberrations and light scattering. We present a STED system that overcomes these challenges. Through the combination of two-photon excitation, adaptive optics, red-emitting organic dyes, and a long-working-distance water-immersion objective lens, our system achieves aberration-corrected 3D super-resolution imaging, which we demonstrate 164 µm deep in fixed mouse brain tissue and 76 µm deep in the brain of a living mouse.
Author Velasco, Mary Grace M
Bewersdorf, Joerg
Barentine, Andrew E S
Zhang, Mengyang
Allgeyer, Edward S
Grutzendler, Jaime
Yuan, Peng
Kidd, Phylicia
M'Saad, Ons
Antonello, Jacopo
May, Dennis
Booth, Martin J
Greco, Valentina
Author_xml – sequence: 1
  givenname: Mary Grace M
  surname: Velasco
  fullname: Velasco, Mary Grace M
  organization: Department of Cell Biology, Yale School of Medicine, New Haven, Connecticut 06520, USA
– sequence: 2
  givenname: Mengyang
  surname: Zhang
  fullname: Zhang, Mengyang
  organization: Department of Neuroscience, Yale School of Medicine, New Haven, Connecticut 06520, USA
– sequence: 3
  givenname: Jacopo
  orcidid: 0000-0001-5486-315X
  surname: Antonello
  fullname: Antonello, Jacopo
  organization: Department of Engineering Science, University of Oxford, Oxford OX1 3PJ, UK
– sequence: 4
  givenname: Peng
  surname: Yuan
  fullname: Yuan, Peng
  organization: Current Address: Department of Biology, Stanford University, Stanford, California 94304, USA
– sequence: 5
  givenname: Edward S
  surname: Allgeyer
  fullname: Allgeyer, Edward S
  organization: Current Address: The Gurdon Institute, University of Cambridge, Cambridge CB21QN, UK
– sequence: 6
  givenname: Dennis
  surname: May
  fullname: May, Dennis
  organization: Department of Genetics, Yale School of Medicine, New Haven, Connecticut 06520, USA
– sequence: 7
  givenname: Ons
  surname: M'Saad
  fullname: M'Saad, Ons
  organization: Department of Cell Biology, Yale School of Medicine, New Haven, Connecticut 06520, USA
– sequence: 8
  givenname: Phylicia
  surname: Kidd
  fullname: Kidd, Phylicia
  organization: Department of Cell Biology, Yale School of Medicine, New Haven, Connecticut 06520, USA
– sequence: 9
  givenname: Andrew E S
  surname: Barentine
  fullname: Barentine, Andrew E S
  organization: Department of Cell Biology, Yale School of Medicine, New Haven, Connecticut 06520, USA
– sequence: 10
  givenname: Valentina
  surname: Greco
  fullname: Greco, Valentina
  organization: Department of Dermatology, Yale Stem Cell Center, Yale Cancer Center, Yale School of Medicine, New Haven, Connecticut 06520, USA
– sequence: 11
  givenname: Jaime
  surname: Grutzendler
  fullname: Grutzendler, Jaime
  organization: Department of Neurology, Yale School of Medicine, New Haven, Connecticut 06520, USA
– sequence: 12
  givenname: Martin J
  orcidid: 0000-0002-9525-8981
  surname: Booth
  fullname: Booth, Martin J
  organization: Department of Engineering Science, University of Oxford, Oxford OX1 3PJ, UK
– sequence: 13
  givenname: Joerg
  surname: Bewersdorf
  fullname: Bewersdorf, Joerg
  organization: Department of Cell Biology, Yale School of Medicine, New Haven, Connecticut 06520, USA
BackLink https://www.ncbi.nlm.nih.gov/pubmed/34239948$$D View this record in MEDLINE/PubMed
BookMark eNpVkM1Lw0AQxRep2Fp79Co5eknd72QvgtRPKNRDPS-bzaSuJNmYTQr-96a0lnqaB_PjzZt3iUa1rwGha4LnhEl-55vOWTPnRKacnKEJZYzHVDA5OtFjNAvhC2NMGMdC4Qs0ZpwypXg6QZI9RqFvoI1bCL7sO-frKAdo4s6F0EPkKrNx9SZydVS67U5VzsIVOi9MGWB2mFP08fy0XrzGy9XL2-JhGVuuki7m1BJgSuRUUJKnOMPEKiPSTOU0JQnNqYUss0lRMJGbJCugUEoQmStlpOIJm6L7vW_TZxXkFuquNaVu2iFW-6O9cfr_pnafeuO3OqWciWRncHswaP13D6HTlQsWytLU4PugqRCYSpkqNqDxHrWtD6GF4niGYL2rW6_e18Nzel_3wN-cZjvSf-WyXy17fcc
CitedBy_id crossref_primary_10_1364_OE_434260
crossref_primary_10_1063_5_0075012
crossref_primary_10_1364_BOE_510512
crossref_primary_10_3788_CJL231342
crossref_primary_10_1088_1361_6463_abff7b
crossref_primary_10_1109_TMI_2024_3358191
crossref_primary_10_1364_AO_416585
crossref_primary_10_1109_JLT_2024_3355177
crossref_primary_10_3389_fchem_2021_746900
crossref_primary_10_1002_glia_24103
crossref_primary_10_1016_j_isci_2022_104961
crossref_primary_10_1038_s41592_024_02236_3
crossref_primary_10_1073_pnas_2201861119
crossref_primary_10_1038_s42005_024_01653_2
crossref_primary_10_1364_BOE_444525
crossref_primary_10_1016_j_neures_2021_11_011
crossref_primary_10_1364_BOE_504764
crossref_primary_10_1371_journal_pone_0290550
crossref_primary_10_1117_1_APN_2_1_016009
crossref_primary_10_1017_S155192952200089X
crossref_primary_10_3788_IRLA20240011
crossref_primary_10_1523_JNEUROSCI_1125_22_2022
crossref_primary_10_3389_fimmu_2023_1176594
crossref_primary_10_1063_5_0102527
crossref_primary_10_1088_1361_6501_ace731
crossref_primary_10_1364_BOE_524013
crossref_primary_10_3788_IRLA20220546
crossref_primary_10_1016_j_nbd_2021_105420
crossref_primary_10_1038_s41467_022_28904_6
crossref_primary_10_1016_j_molcel_2021_12_022
crossref_primary_10_1038_s41592_023_01936_6
crossref_primary_10_1063_5_0066331
crossref_primary_10_1364_AO_455628
crossref_primary_10_1021_acsphotonics_2c00957
crossref_primary_10_1038_s41598_022_13377_w
crossref_primary_10_1088_1402_4896_acdf8e
crossref_primary_10_1117_1_NPh_11_1_014415
Cites_doi 10.1016/j.bpj.2011.07.027
10.1038/nrm.2017.71
10.1016/j.cell.2015.06.067
10.1103/PhysRevLett.88.163901
10.1038/nchem.1546
10.1364/OE.18.001657
10.1073/pnas.97.15.8206
10.1073/pnas.1807104115
10.1002/jbio.201300041
10.1016/j.bpj.2018.07.028
10.1007/978-1-4939-7265-4_5
10.1364/OE.27.023378
10.1083/jcb.201809107
10.1364/OPTICA.1.000181
10.1111/j.1365-2818.2005.01431.x
10.1088/1361-6463/abac81
10.1038/nmeth.4403
10.1016/j.bpj.2011.05.020
10.1364/OPEX.12.003605
10.1364/OE.24.008862
10.1038/ncomms10778
10.1364/OE.20.020998
10.1038/s41592-018-0145-5
10.1364/JOSA.66.000207
10.1016/j.bpj.2012.12.053
10.1021/nl901398t
10.1021/cb800025k
10.1534/genetics.118.300227
10.1038/nmeth818
10.1038/lsa.2014.46
10.1038/nmeth.4337
10.1364/BOE.10.001999
10.1364/OL.24.000954
10.1038/nmeth.1214
10.1021/acsphotonics.0c00388
10.1111/j.1365-2818.2004.01267.x
10.1038/nmeth.2925
10.1038/ncomms8276
10.1364/OL.19.000780
10.1073/pnas.1819965116
10.1073/pnas.1119129109
10.1364/OPEX.12.006540
10.1038/nbt899
10.1364/BOE.9.003624
10.1126/science.1215369
10.1038/srep15348
10.1007/BF02956173
10.1016/j.cell.2018.02.007
10.1364/OL.40.004915
10.1364/BOE.2.003135
10.1364/OE.17.014567
10.1364/BOE.8.003891
10.1016/j.ymeth.2019.05.020
10.1063/1.5020249
10.1021/acsnano.9b05891
ContentType Journal Article
Copyright Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI.
Published by The Optical Society under the terms of the . Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI. 2021
Copyright_xml – notice: Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI.
– notice: Published by The Optical Society under the terms of the . Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI. 2021
DBID NPM
AAYXX
CITATION
7X8
5PM
DOI 10.1364/optica.416841
DatabaseName PubMed
CrossRef
MEDLINE - Academic
PubMed Central (Full Participant titles)
DatabaseTitle PubMed
CrossRef
MEDLINE - Academic
DatabaseTitleList
CrossRef
PubMed
Database_xml – sequence: 1
  dbid: NPM
  name: PubMed
  url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
DeliveryMethod fulltext_linktorsrc
Discipline Physics
EISSN 2334-2536
EndPage 450
ExternalDocumentID 10_1364_OPTICA_416841
34239948
Genre Journal Article
GrantInformation_xml – fundername: NIDDK NIH HHS
  grantid: P30 DK045735
– fundername: Wellcome Trust
  grantid: 203285/Z/16/Z
– fundername: National Institutes of Health
  grantid: 1R01NS089734; P30-DK45735; U01 DA047734
– fundername: Wellcome Trust
  grantid: 203285/B/16/Z; 203285/C/16/Z
– fundername: Yale’s Integrated Graduate Program in Physical and Engineering Biology
– fundername: European Research Council
  grantid: AdOMIS 695140
– fundername: G. Harold and Leila Y. Mathers Foundation
GroupedDBID 4.4
AAWJZ
ADBBV
AENEX
AKGWG
ALMA_UNASSIGNED_HOLDINGS
AZSQR
BCNDV
DSZJF
EBS
GROUPED_DOAJ
M~E
NPM
OFLFD
OK1
OPJBK
ROL
ROP
ROS
TR6
AAYXX
CITATION
7X8
5PM
ID FETCH-LOGICAL-c497t-42c1e395d2521d80b01c9a58b9d28172d2cebbc7ff35da7bfef99516d99a69473
ISSN 2334-2536
IngestDate Tue Apr 09 21:53:18 EDT 2024
Fri Apr 12 06:19:32 EDT 2024
Wed Aug 07 14:11:41 EDT 2024
Thu May 23 23:32:00 EDT 2024
IsDoiOpenAccess false
IsOpenAccess true
IsPeerReviewed true
IsScholarly true
Issue 4
Language English
License Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI.
https://creativecommons.org/licenses/by/4.0
2334-2536/21/040442-09
LinkModel OpenURL
MergedId FETCHMERGED-LOGICAL-c497t-42c1e395d2521d80b01c9a58b9d28172d2cebbc7ff35da7bfef99516d99a69473
Notes ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ORCID 0000-0001-5486-315X
0000-0002-9525-8981
OpenAccessLink http://dx.doi.org/10.1364/OPTICA.416841
PMID 34239948
PQID 2550266893
PQPubID 23479
PageCount 9
ParticipantIDs pubmedcentral_primary_oai_pubmedcentral_nih_gov_8243577
proquest_miscellaneous_2550266893
crossref_primary_10_1364_OPTICA_416841
pubmed_primary_34239948
PublicationCentury 2000
PublicationDate 2021-04-20
PublicationDateYYYYMMDD 2021-04-20
PublicationDate_xml – month: 04
  year: 2021
  text: 2021-04-20
  day: 20
PublicationDecade 2020
PublicationPlace United States
PublicationPlace_xml – name: United States
PublicationTitle Optica
PublicationTitleAlternate Optica
PublicationYear 2021
Publisher Optical Society of America
Publisher_xml – name: Optical Society of America
References Klar (optica-8-4-442-R11) 1999; 24
Abbe (optica-8-4-442-R2) 1873; 9
Turcotte (optica-8-4-442-R42) 2019; 116
Zheng (optica-8-4-442-R41) 2017; 14
Moneron (optica-8-4-442-R25) 2009; 17
Kilian (optica-8-4-442-R53) 2018; 15
Helmchen (optica-8-4-442-R23) 2005; 2
Winter (optica-8-4-442-R24) 2014; 1
Wang (optica-8-4-442-R40) 2014; 11
Schwertner (optica-8-4-442-R31) 2005; 217
Zdankowski (optica-8-4-442-R38) 2020; 14
Lukinavicius (optica-8-4-442-R51) 2013; 5
Barbotin (optica-8-4-442-R20) 2020; 7
Schroeder (optica-8-4-442-R3) 2018; 218
Lenz (optica-8-4-442-R17) 2014; 7
Aviles-Espinosa (optica-8-4-442-R39) 2011; 2
Tonnesen (optica-8-4-442-R55) 2018; 172
Gould (optica-8-4-442-R12) 2012; 20
Galiñanes (optica-8-4-442-R50) 2018; 9
Bianchini (optica-8-4-442-R28) 2012; 109
Masch (optica-8-4-442-R48) 2018; 115
Velasco (optica-8-4-442-R29) 2015; 40
Takasaki (optica-8-4-442-R27) 2013; 104
Hell (optica-8-4-442-R5) 1994; 19
Heine (optica-8-4-442-R22) 2018; 89
Bottanelli (optica-8-4-442-R44) 2016; 7
Ter Veer (optica-8-4-442-R26) 2017; 1663
Booth (optica-8-4-442-R33) 2014; 3
Zdankowski (optica-8-4-442-R37) 2019; 10
Zipfel (optica-8-4-442-R46) 2003; 21
Rankin (optica-8-4-442-R7) 2011; 100
Los (optica-8-4-442-R49) 2008; 3
Barbotin (optica-8-4-442-R19) 2019; 27
Klar (optica-8-4-442-R16) 2000; 97
Grimm (optica-8-4-442-R52) 2017; 14
Török (optica-8-4-442-R10) 2004; 12
Stockhammer (optica-8-4-442-R4) 2020; 54
Deng (optica-8-4-442-R18) 2010; 18
Berning (optica-8-4-442-R9) 2012; 335
Sahl (optica-8-4-442-R1) 2017; 18
Schwertner (optica-8-4-442-R34) 2004; 12
Richardson (optica-8-4-442-R30) 2015; 162
Noll (optica-8-4-442-R47) 1976; 66
Waldchen (optica-8-4-442-R54) 2015; 5
Schmidt (optica-8-4-442-R14) 2009; 9
Urban (optica-8-4-442-R21) 2011; 101
Patton (optica-8-4-442-R36) 2016; 24
Dunst (optica-8-4-442-R6) 2019; 211
Wang (optica-8-4-442-R43) 2015; 6
Turcotte (optica-8-4-442-R32) 2017; 8
Steffens (optica-8-4-442-R8) 2019; 174
Schmidt (optica-8-4-442-R15) 2008; 5
Schwertner (optica-8-4-442-R35) 2004; 213
Barentine (optica-8-4-442-R45) 2018; 115
Dyba (optica-8-4-442-R13) 2002; 88
References_xml – volume: 101
  start-page: 1277
  year: 2011
  ident: optica-8-4-442-R21
  publication-title: Biophys. J.
  doi: 10.1016/j.bpj.2011.07.027
  contributor:
    fullname: Urban
– volume: 18
  start-page: 685
  year: 2017
  ident: optica-8-4-442-R1
  publication-title: Nat. Rev. Mol. Cell Biol.
  doi: 10.1038/nrm.2017.71
  contributor:
    fullname: Sahl
– volume: 162
  start-page: 246
  year: 2015
  ident: optica-8-4-442-R30
  publication-title: Cell
  doi: 10.1016/j.cell.2015.06.067
  contributor:
    fullname: Richardson
– volume: 88
  start-page: 163901
  year: 2002
  ident: optica-8-4-442-R13
  publication-title: Phys. Rev. Lett.
  doi: 10.1103/PhysRevLett.88.163901
  contributor:
    fullname: Dyba
– volume: 5
  start-page: 132
  year: 2013
  ident: optica-8-4-442-R51
  publication-title: Nature Chem.
  doi: 10.1038/nchem.1546
  contributor:
    fullname: Lukinavicius
– volume: 18
  start-page: 1657
  year: 2010
  ident: optica-8-4-442-R18
  publication-title: Opt. Express
  doi: 10.1364/OE.18.001657
  contributor:
    fullname: Deng
– volume: 97
  start-page: 8206
  year: 2000
  ident: optica-8-4-442-R16
  publication-title: Proc. Natl. Acad. Sci. USA
  doi: 10.1073/pnas.97.15.8206
  contributor:
    fullname: Klar
– volume: 115
  start-page: E8047
  year: 2018
  ident: optica-8-4-442-R48
  publication-title: Proc. Natl. Acad. Sci. U.S.A.
  doi: 10.1073/pnas.1807104115
  contributor:
    fullname: Masch
– volume: 7
  start-page: 29
  year: 2014
  ident: optica-8-4-442-R17
  publication-title: J. Biophoton.
  doi: 10.1002/jbio.201300041
  contributor:
    fullname: Lenz
– volume: 115
  start-page: 951
  year: 2018
  ident: optica-8-4-442-R45
  publication-title: Biophys. J.
  doi: 10.1016/j.bpj.2018.07.028
  contributor:
    fullname: Barentine
– volume: 1663
  start-page: 45
  year: 2017
  ident: optica-8-4-442-R26
  publication-title: Methods Mol. Biol.
  doi: 10.1007/978-1-4939-7265-4_5
  contributor:
    fullname: Ter Veer
– volume: 27
  start-page: 23378
  year: 2019
  ident: optica-8-4-442-R19
  publication-title: Opt. Express
  doi: 10.1364/OE.27.023378
  contributor:
    fullname: Barbotin
– volume: 218
  start-page: 83
  year: 2018
  ident: optica-8-4-442-R3
  publication-title: J. Cell Biol.
  doi: 10.1083/jcb.201809107
  contributor:
    fullname: Schroeder
– volume: 1
  start-page: 181
  year: 2014
  ident: optica-8-4-442-R24
  publication-title: Optica
  doi: 10.1364/OPTICA.1.000181
  contributor:
    fullname: Winter
– volume: 217
  start-page: 184
  year: 2005
  ident: optica-8-4-442-R31
  publication-title: J. Microsc.
  doi: 10.1111/j.1365-2818.2005.01431.x
  contributor:
    fullname: Schwertner
– volume: 54
  start-page: 033001
  year: 2020
  ident: optica-8-4-442-R4
  publication-title: J. Phys. D
  doi: 10.1088/1361-6463/abac81
  contributor:
    fullname: Stockhammer
– volume: 14
  start-page: 987
  year: 2017
  ident: optica-8-4-442-R52
  publication-title: Nat. Methods
  doi: 10.1038/nmeth.4403
  contributor:
    fullname: Grimm
– volume: 100
  start-page: L63
  year: 2011
  ident: optica-8-4-442-R7
  publication-title: Biophys. J.
  doi: 10.1016/j.bpj.2011.05.020
  contributor:
    fullname: Rankin
– volume: 12
  start-page: 3605
  year: 2004
  ident: optica-8-4-442-R10
  publication-title: Opt. Express
  doi: 10.1364/OPEX.12.003605
  contributor:
    fullname: Török
– volume: 24
  start-page: 8862
  year: 2016
  ident: optica-8-4-442-R36
  publication-title: Opt. Express
  doi: 10.1364/OE.24.008862
  contributor:
    fullname: Patton
– volume: 7
  start-page: 10778
  year: 2016
  ident: optica-8-4-442-R44
  publication-title: Nat. Commun.
  doi: 10.1038/ncomms10778
  contributor:
    fullname: Bottanelli
– volume: 20
  start-page: 20998
  year: 2012
  ident: optica-8-4-442-R12
  publication-title: Opt. Express
  doi: 10.1364/OE.20.020998
  contributor:
    fullname: Gould
– volume: 15
  start-page: 755
  year: 2018
  ident: optica-8-4-442-R53
  publication-title: Nat. Methods
  doi: 10.1038/s41592-018-0145-5
  contributor:
    fullname: Kilian
– volume: 66
  start-page: 207
  year: 1976
  ident: optica-8-4-442-R47
  publication-title: J. Opt. Soc. Am.
  doi: 10.1364/JOSA.66.000207
  contributor:
    fullname: Noll
– volume: 104
  start-page: 770
  year: 2013
  ident: optica-8-4-442-R27
  publication-title: Biophys. J.
  doi: 10.1016/j.bpj.2012.12.053
  contributor:
    fullname: Takasaki
– volume: 9
  start-page: 2508
  year: 2009
  ident: optica-8-4-442-R14
  publication-title: Nano Lett.
  doi: 10.1021/nl901398t
  contributor:
    fullname: Schmidt
– volume: 3
  start-page: 373
  year: 2008
  ident: optica-8-4-442-R49
  publication-title: ACS Chem. Biol.
  doi: 10.1021/cb800025k
  contributor:
    fullname: Los
– volume: 211
  start-page: 15
  year: 2019
  ident: optica-8-4-442-R6
  publication-title: Genetics
  doi: 10.1534/genetics.118.300227
  contributor:
    fullname: Dunst
– volume: 2
  start-page: 932
  year: 2005
  ident: optica-8-4-442-R23
  publication-title: Nat. Methods
  doi: 10.1038/nmeth818
  contributor:
    fullname: Helmchen
– volume: 3
  start-page: e165
  year: 2014
  ident: optica-8-4-442-R33
  publication-title: Light Sci. Appl.
  doi: 10.1038/lsa.2014.46
  contributor:
    fullname: Booth
– volume: 14
  start-page: 869
  year: 2017
  ident: optica-8-4-442-R41
  publication-title: Nat. Methods
  doi: 10.1038/nmeth.4337
  contributor:
    fullname: Zheng
– volume: 10
  start-page: 1999
  year: 2019
  ident: optica-8-4-442-R37
  publication-title: Biomed. Opt. Express
  doi: 10.1364/BOE.10.001999
  contributor:
    fullname: Zdankowski
– volume: 24
  start-page: 954
  year: 1999
  ident: optica-8-4-442-R11
  publication-title: Opt. Lett.
  doi: 10.1364/OL.24.000954
  contributor:
    fullname: Klar
– volume: 5
  start-page: 539
  year: 2008
  ident: optica-8-4-442-R15
  publication-title: Nat. Methods
  doi: 10.1038/nmeth.1214
  contributor:
    fullname: Schmidt
– volume: 7
  start-page: 1742
  year: 2020
  ident: optica-8-4-442-R20
  publication-title: ACS Photon.
  doi: 10.1021/acsphotonics.0c00388
  contributor:
    fullname: Barbotin
– volume: 213
  start-page: 11
  year: 2004
  ident: optica-8-4-442-R35
  publication-title: J. Microsc.
  doi: 10.1111/j.1365-2818.2004.01267.x
  contributor:
    fullname: Schwertner
– volume: 11
  start-page: 625
  year: 2014
  ident: optica-8-4-442-R40
  publication-title: Nat. Methods
  doi: 10.1038/nmeth.2925
  contributor:
    fullname: Wang
– volume: 6
  start-page: 7276
  year: 2015
  ident: optica-8-4-442-R43
  publication-title: Nat. Commun.
  doi: 10.1038/ncomms8276
  contributor:
    fullname: Wang
– volume: 19
  start-page: 780
  year: 1994
  ident: optica-8-4-442-R5
  publication-title: Opt. Lett.
  doi: 10.1364/OL.19.000780
  contributor:
    fullname: Hell
– volume: 116
  start-page: 9586
  year: 2019
  ident: optica-8-4-442-R42
  publication-title: Proc. Natl. Acad. Sci. USA
  doi: 10.1073/pnas.1819965116
  contributor:
    fullname: Turcotte
– volume: 109
  start-page: 6390
  year: 2012
  ident: optica-8-4-442-R28
  publication-title: Proc. Natl. Acad. Sci. USA
  doi: 10.1073/pnas.1119129109
  contributor:
    fullname: Bianchini
– volume: 12
  start-page: 6540
  year: 2004
  ident: optica-8-4-442-R34
  publication-title: Opt. Express
  doi: 10.1364/OPEX.12.006540
  contributor:
    fullname: Schwertner
– volume: 21
  start-page: 1369
  year: 2003
  ident: optica-8-4-442-R46
  publication-title: Nat. Biotechnol.
  doi: 10.1038/nbt899
  contributor:
    fullname: Zipfel
– volume: 9
  start-page: 3624
  year: 2018
  ident: optica-8-4-442-R50
  publication-title: Biomed. Opt. Express
  doi: 10.1364/BOE.9.003624
  contributor:
    fullname: Galiñanes
– volume: 335
  start-page: 551
  year: 2012
  ident: optica-8-4-442-R9
  publication-title: Science
  doi: 10.1126/science.1215369
  contributor:
    fullname: Berning
– volume: 5
  start-page: 15348
  year: 2015
  ident: optica-8-4-442-R54
  publication-title: Sci. Rep.
  doi: 10.1038/srep15348
  contributor:
    fullname: Waldchen
– volume: 9
  start-page: 413
  year: 1873
  ident: optica-8-4-442-R2
  publication-title: Archiv für mikroskopische Anatomie
  doi: 10.1007/BF02956173
  contributor:
    fullname: Abbe
– volume: 172
  start-page: 1108
  year: 2018
  ident: optica-8-4-442-R55
  publication-title: Cell
  doi: 10.1016/j.cell.2018.02.007
  contributor:
    fullname: Tonnesen
– volume: 40
  start-page: 4915
  year: 2015
  ident: optica-8-4-442-R29
  publication-title: Opt. Lett.
  doi: 10.1364/OL.40.004915
  contributor:
    fullname: Velasco
– volume: 2
  start-page: 3135
  year: 2011
  ident: optica-8-4-442-R39
  publication-title: Biomed. Opt. Express
  doi: 10.1364/BOE.2.003135
  contributor:
    fullname: Aviles-Espinosa
– volume: 17
  start-page: 14567
  year: 2009
  ident: optica-8-4-442-R25
  publication-title: Opt. Express
  doi: 10.1364/OE.17.014567
  contributor:
    fullname: Moneron
– volume: 8
  start-page: 3891
  year: 2017
  ident: optica-8-4-442-R32
  publication-title: Biomed. Opt. Express
  doi: 10.1364/BOE.8.003891
  contributor:
    fullname: Turcotte
– volume: 174
  start-page: 42
  year: 2019
  ident: optica-8-4-442-R8
  publication-title: Methods
  doi: 10.1016/j.ymeth.2019.05.020
  contributor:
    fullname: Steffens
– volume: 89
  start-page: 053701
  year: 2018
  ident: optica-8-4-442-R22
  publication-title: Rev. Sci. Instrum.
  doi: 10.1063/1.5020249
  contributor:
    fullname: Heine
– volume: 14
  start-page: 394
  year: 2020
  ident: optica-8-4-442-R38
  publication-title: ACS Nano
  doi: 10.1021/acsnano.9b05891
  contributor:
    fullname: Zdankowski
SSID ssj0001340590
Score 2.4748075
Snippet Stimulated emission depletion (STED) microscopy enables the three-dimensional (3D) visualization of dynamic nanoscale structures in living cells, offering...
SourceID pubmedcentral
proquest
crossref
pubmed
SourceType Open Access Repository
Aggregation Database
Index Database
StartPage 442
Title 3D super-resolution deep-tissue imaging in living mice
URI https://www.ncbi.nlm.nih.gov/pubmed/34239948
https://search.proquest.com/docview/2550266893
https://pubmed.ncbi.nlm.nih.gov/PMC8243577
Volume 8
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3db9MwELdgCIkXxDflS0FCvFQpqe3Y8eO0AQMJtocO7S1y_KFFYmnUpg_bX7-z46ZJ24fBS1S5iWvdXS-_O9_9jNAnk9hUcQlhCUu522ZMYqmnLGaJLTQ1Elvu-p1__WYn5_TnRXqxOW3Ud5c0xUTd7O0r-R-twhjo1XXJ_oNmu0lhAD6DfuEKGobrnXRMjsfLVW0WMcTM4YfG2pg6brw4x-VVewZRWY3_lj5zcBVK3dZ49LRuesU6fwxgaTUPPTzX4-8L6f72k53ssquFvZbhpeeTCI7Te72NA062nnfuZNWmWM9MuD2kGPDU7ZbgZOOJMCEwkJLAWb1nLLjSrGcxtOcWacugteOuCaMg49Oz2Y-jwwlAw6wlwRrSYm-9rroiQr8Xx2jePp63j99HDzAXadoLrn22jVDXZetPGgzrDoSrMMOXwQKGAGUn6tgunu2hkdkT9DiEEdFhaxNP0T1TPUMPfTmvWj5HjBxH25YR9SwjCpYRlVXUWkbkLOMFOv_2dXZ0EocTMmJFBW9iitXUEJFqDChMZy6prYRMs0JonAE01ViZolDcWpJqyQtrrABIzbQQkgnKyUt0UIF9vEaRoZYLRw1ECYDMREkFUM5myhKWZFokI_R5LZa8bolQ8r0aGKGPa6Hl4Krc_pOszHy1zCF6hYifAUIeoVetELupPBGloNkI8YF4uxscDfrwm6q89HToGQbIz_mbuy7wLXq0MfJ36KBZrMx7QJZN8cEbzS3wEXat
link.rule.ids 230,315,786,790,870,891,27955,27956
linkProvider Directory of Open Access Journals
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=3D+super-resolution+deep-tissue+imaging+in+living+mice&rft.jtitle=Optica&rft.au=Velasco%2C+Mary+Grace+M.&rft.au=Zhang%2C+Mengyang&rft.au=Antonello%2C+Jacopo&rft.au=Yuan%2C+Peng&rft.date=2021-04-20&rft.issn=2334-2536&rft.eissn=2334-2536&rft.volume=8&rft.issue=4&rft.spage=442&rft_id=info:doi/10.1364%2FOPTICA.416841&rft.externalDBID=n%2Fa&rft.externalDocID=10_1364_OPTICA_416841
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=2334-2536&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=2334-2536&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=2334-2536&client=summon