Identification of RNA-binding protein targets with HyperTRIBE

• Published in: Nature protocols Vol. 13; no. 8; pp. 1829 - 1849
• Main Authors: Rahman, Reazur, Xu, Weijin, Jin, Hua, Rosbash, Michael
• Format: Journal Article
• Language: English
• Published: England Nature Publishing Group 01.08.2018
• Subjects: Adenosine; Adenosine deaminase; Adenosine Deaminase - genetics; Adenosine Deaminase - metabolism; Animals; Bias; Binding proteins; Binding Sites; Biosynthesis; Catalysis; Cell Line; Computation; Computer applications; Death; Drosophila; Editing; Enzymes; Evolution; Fruit flies; Fusion protein; Gene targeting; Identification and classification; Methods; Molecular biology; Mutant Proteins - genetics; Mutant Proteins - metabolism; Next-generation sequencing; Novels; Prejudice; Protein Binding; Proteins; Recombinant Fusion Proteins - genetics; Recombinant Fusion Proteins - metabolism; Ribonucleic acid; RNA; RNA - metabolism; RNA editing; RNA sequencing; RNA-binding protein; RNA-Binding Proteins - metabolism; Software; Target detection; Target recognition; United States

RNA-binding proteins (RBPs) accompany RNA from birth to death, affecting RNA biogenesis and functions. Identifying RBP-RNA interactions is essential to understanding their complex roles in different cellular processes. However, detecting in vivo RNA targets of RBPs, especially in a small number of d...