Localization of key amino acid residues in the dominant conformational epitopes on thyroid peroxidase recognized by mouse monoclonal antibodies

• Published in: Autoimmunity (Chur, Switzerland) Vol. 45; no. 6; pp. 476 - 484
• Main Authors: Godlewska, Marlena, Czarnocka, Barbara, Gora, Monika
• Format: Journal Article
• Language: English
• Published: England Informa Healthcare 01.09.2012; Taylor & Francis
• Subjects: Animals; Antibodies, Monoclonal - immunology; autoimmune thyroid diseases; CHO Cells; Cricetinae; Epitope Mapping; Epitopes - chemistry; Humans; Immunodominant Epitopes - chemistry; immunodominant regions; Iodide Peroxidase - chemistry; Iodide Peroxidase - genetics; Iodide Peroxidase - immunology; Iodide Peroxidase - metabolism; Mice; Models, Molecular; mouse monoclonal antibody; Mutagenesis, Site-Directed; thyroid peroxidase (TPO)

Autoantibodies to thyroid peroxidase (TPO), the major target autoantigen in autoimmune thyroid diseases, recognize conformational epitopes limited to two immunodominant regions (IDRs) termed IDR-A and -B. The apparent restricted heterogeneity of TPO autoantibodies was discovered using TPO-specific m...