Localization of key amino acid residues in the dominant conformational epitopes on thyroid peroxidase recognized by mouse monoclonal antibodies
Autoantibodies to thyroid peroxidase (TPO), the major target autoantigen in autoimmune thyroid diseases, recognize conformational epitopes limited to two immunodominant regions (IDRs) termed IDR-A and -B. The apparent restricted heterogeneity of TPO autoantibodies was discovered using TPO-specific m...
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Published in | Autoimmunity (Chur, Switzerland) Vol. 45; no. 6; pp. 476 - 484 |
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Format | Journal Article |
Language | English |
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Informa Healthcare
01.09.2012
Taylor & Francis |
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Abstract | Autoantibodies to thyroid peroxidase (TPO), the major target autoantigen in autoimmune thyroid diseases, recognize conformational epitopes limited to two immunodominant regions (IDRs) termed IDR-A and -B. The apparent restricted heterogeneity of TPO autoantibodies was discovered using TPO-specific mouse monoclonal antibodies (mAbs) and later confirmed by human recombinant Fabs. In earlier studies we identified key amino acids crucial for the interaction of human autoantibodies with TPO. Here we show the critical residues that participate in binding of five mAbs to the conformational epitopes on the TPO surface. Using ELISA we tested the reactivity of single and multiple TPO mutants expressed in CHO cells with a panel of mAbs specifically recognizing IDR-A (mAb 2 and 9) and IDR-B (mAb 15, 18, 64). We show that antibodies recognizing very similar regions on the TPO surface may interact with different sets of residues. We found that residues K713 and E716 contribute to the interaction between mAb 2 and TPO. The epitope for mAb 9 is critically dependent on residues R646 and E716. Moreover, we demonstrate that amino acids E604 and D630 are part of the functional epitope for mAb 15, and amino acids D624 and K627 for mAb 18. Finally, residues E604, D620, D624, K627, and D630 constitute the epitope for mAb 64. This is the first detailed study identifying the key resides for binding of mAbs 2, 9, 15, 18, and 64. Better understanding of those antibodies' specificity will be helpful in elucidating the properties of TPO as an antigen in autoimmune disorders. |
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AbstractList | Autoantibodies to thyroid peroxidase (TPO), the major target autoantigen in autoimmune thyroid diseases, recognize conformational epitopes limited to two immunodominant regions (IDRs) termed IDR-A and -B. The apparent restricted heterogeneity of TPO autoantibodies was discovered using TPO-specific mouse monoclonal antibodies (mAbs) and later confirmed by human recombinant Fabs. In earlier studies we identified key amino acids crucial for the interaction of human autoantibodies with TPO. Here we show the critical residues that participate in binding of five mAbs to the conformational epitopes on the TPO surface. Using ELISA we tested the reactivity of single and multiple TPO mutants expressed in CHO cells with a panel of mAbs specifically recognizing IDR-A (mAb 2 and 9) and IDR-B (mAb 15, 18, 64). We show that antibodies recognizing very similar regions on the TPO surface may interact with different sets of residues. We found that residues K713 and E716 contribute to the interaction between mAb 2 and TPO. The epitope for mAb 9 is critically dependent on residues R646 and E716. Moreover, we demonstrate that amino acids E604 and D630 are part of the functional epitope for mAb 15, and amino acids D624 and K627 for mAb 18. Finally, residues E604, D620, D624, K627, and D630 constitute the epitope for mAb 64. This is the first detailed study identifying the key resides for binding of mAbs 2, 9, 15, 18, and 64. Better understanding of those antibodies' specificity will be helpful in elucidating the properties of TPO as an antigen in autoimmune disorders. |
Author | Czarnocka, Barbara Gora, Monika Godlewska, Marlena |
Author_xml | – sequence: 1 givenname: Marlena surname: Godlewska fullname: Godlewska, Marlena email: mgora@ibb.waw.pl, mgora@ibb.waw.pl organization: Department of Biochemistry and Molecular Biology, Medical Centre of Postgraduate Education – sequence: 2 givenname: Barbara surname: Czarnocka fullname: Czarnocka, Barbara email: mgora@ibb.waw.pl, mgora@ibb.waw.pl organization: Department of Biochemistry and Molecular Biology, Medical Centre of Postgraduate Education – sequence: 3 givenname: Monika surname: Gora fullname: Gora, Monika email: mgora@ibb.waw.pl, mgora@ibb.waw.pl organization: Department of Genetics, Institute of Biochemistry and Biophysics, Polish Academy of Sciences |
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References_xml | – volume: 133 start-page: 2496 year: 1993 ident: CIT0012 publication-title: Endocrinology doi: 10.1210/endo.133.6.8243270 contributor: fullname: Nishikawa T – volume: 37 start-page: 211 year: 2004 ident: CIT0006 publication-title: Autoimmunity doi: 10.1080/0891693042000193339 contributor: fullname: Jastrzębska-Bohaterewicz E – volume: 147 start-page: 5995 year: 2006 ident: CIT0027 publication-title: Endocrinology doi: 10.1210/en.2006-0912 contributor: fullname: Dubska M – volume: 11 start-page: 141 year: 1992 ident: CIT0036 publication-title: Autoimmunity doi: 10.3109/08916939209035148 contributor: fullname: Ewins DL – volume: 295 start-page: 1118 year: 2002 ident: CIT0020 publication-title: Biochem Biophys Res Commun doi: 10.1016/S0006-291X(02)00827-6 contributor: fullname: Blanchin S – volume: 226 start-page: 851 year: 1992 ident: CIT0037 publication-title: J Mol Biol doi: 10.1016/0022-2836(92)90636-X contributor: fullname: Jin L – volume: 278 start-page: 9560 year: 2003 ident: CIT0023 publication-title: J Biol Chem doi: 10.1074/jbc.M211930200 contributor: fullname: Bresson D – volume: 146 start-page: 2834 year: 2005 ident: CIT0029 publication-title: Endocrinology doi: 10.1210/en.2005-0038 contributor: fullname: Bresson D – volume: 125 start-page: 1211 year: 1989 ident: CIT0005 publication-title: Endocrinology doi: 10.1210/endo-125-3-1211 contributor: fullname: Ruf J – volume: 136 start-page: 137 year: 2004 ident: CIT0030 publication-title: Clin Exp Immunol doi: 10.1111/j.1365-2249.2004.02422.x contributor: fullname: Gora M – volume: 38 start-page: 605 year: 2005 ident: CIT0033 publication-title: Autoimmunity doi: 10.1080/08916930500315084 contributor: fullname: Bossowski A – volume: 2 start-page: 733 year: 1994 ident: CIT0038 publication-title: Structure doi: 10.1016/S0969-2126(00)00074-5 contributor: fullname: Malby RL – volume: 11 start-page: 227 year: 2001 ident: CIT0019 publication-title: Thyroid doi: 10.1089/105072501750159598 contributor: fullname: Xiong Z – ident: CIT0043 doi: 10.1677/joe.0.160R013 – volume: 79 start-page: 1648 year: 1994 ident: CIT0013 publication-title: J Clin Endocrinol Metab contributor: fullname: Nishikawa T – volume: 190 start-page: 147 year: 1985 ident: CIT0001 publication-title: FEBS Lett doi: 10.1016/0014-5793(85)80446-4 contributor: fullname: Czarnocka B – volume: 273 start-page: 8056 year: 1998 ident: CIT0011 publication-title: J Biol Chem doi: 10.1074/jbc.273.14.8056 contributor: fullname: Estienne V – volume: 71 start-page: 53 year: 1990 ident: CIT0031 publication-title: J Clin Endocrinol Metab doi: 10.1210/jcem-71-1-53 contributor: fullname: Finke R – volume: 85 start-page: 875 year: 2001 ident: CIT0032 publication-title: Br J Cancer doi: 10.1054/bjoc.2001.2015 contributor: fullname: Czarnocka B – volume: 330 start-page: 24 year: 1996 ident: CIT0009 publication-title: Arch Biochem Biophys doi: 10.1006/abbi.1996.0222 contributor: fullname: Taurog A – volume: 137 start-page: 1000 year: 1996 ident: CIT0021 publication-title: Endocrinology doi: 10.1210/endo.137.3.8603566 contributor: fullname: Nishikawa T – volume: 320 start-page: 795 year: 2004 ident: CIT0025 publication-title: Biochem Biophys Res Commun doi: 10.1016/j.bbrc.2004.06.028 contributor: fullname: Gora M – volume: 166 start-page: 1327 year: 2001 ident: CIT0028 publication-title: J Immunol doi: 10.4049/jimmunol.166.2.1327 contributor: fullname: Guo J – volume: 18 start-page: 1091 year: 2006 ident: CIT0026 publication-title: Int Immunol doi: 10.1093/intimm/dxl042 contributor: fullname: Rebuffat SA – volume: 234 start-page: 366 year: 1997 ident: CIT0042 publication-title: Biochem Biophys Res Commun doi: 10.1006/bbrc.1997.6600 contributor: fullname: Gardas A – volume: 5 start-page: 237 year: 2000 ident: CIT0015 publication-title: Redox Rep doi: 10.1179/135100000101535681 contributor: fullname: Gardas A – volume: 36 start-page: 85 year: 2009 ident: CIT0003 publication-title: Int J Immunogenet doi: 10.1111/j.1744-313X.2009.00830.x contributor: fullname: Hadj-Kacem H – volume: 23 start-page: 145 year: 1996 ident: CIT0014 publication-title: Autoimmunity doi: 10.3109/08916939608995338 contributor: fullname: Czarnocka B – volume: 17 start-page: 939 year: 2007 ident: CIT0002 publication-title: Thyroid doi: 10.1089/thy.2007.0169 contributor: fullname: McLachlan SM – volume: 30 start-page: 315 year: 2001 ident: CIT0004 publication-title: Endocrinol Metab Clin North Am doi: 10.1016/S0889-8529(05)70189-4 contributor: fullname: Saravanan P – volume: 6 start-page: 257 year: 1990 ident: CIT0016 publication-title: Autoimmunity doi: 10.3109/08916939008998418 contributor: fullname: Banga JP – volume: 141 start-page: 2018 year: 2000 ident: CIT0010 publication-title: Endocrinology doi: 10.1210/endo.141.6.7506 contributor: fullname: Hobby P – volume: 77 start-page: 1715 year: 1993 ident: CIT0007 publication-title: J Clin Endocrinol Metab contributor: fullname: Chazenbalk GD – volume: 14 start-page: 359 year: 2002 ident: CIT0022 publication-title: Int Immunol doi: 10.1093/intimm/14.4.359 contributor: fullname: Estienne V – volume: 45 start-page: 7380 year: 2006 ident: CIT0034 publication-title: Biochemistry doi: 10.1021/bi060415i contributor: fullname: Le Fourn V – volume: 21 start-page: 145 year: 2007 ident: CIT0040 publication-title: BioDrugs doi: 10.2165/00063030-200721030-00002 contributor: fullname: Gershoni JM – volume: 95 start-page: 375 year: 2010 ident: CIT0035 publication-title: J Clin Endocrinol Metab doi: 10.1210/jc.2009-1727 contributor: fullname: Song Y – volume: 271 start-page: 4966 year: 1996 ident: CIT0017 publication-title: J Biol Chem doi: 10.1074/jbc.271.9.4966 contributor: fullname: Arscott PL – volume: 82 start-page: 2639 year: 1997 ident: CIT0008 publication-title: J Clin Endocrinol Metab contributor: fullname: Czarnocka B – ident: CIT0018 doi: 10.3109/08916939908993850 – volume: 9 start-page: 508 year: 1996 ident: CIT0041 publication-title: Methods doi: 10.1006/meth.1996.0058 contributor: fullname: Benjamin DC – volume: 279 start-page: 39058 year: 2004 ident: CIT0024 publication-title: J Biol Chem doi: 10.1074/jbc.M403897200 contributor: fullname: Bresson D – ident: CIT0039 doi: 10.1006/meth.1996.0054 – volume: 111 start-page: 408 year: 1998 ident: CIT0044 publication-title: Clin Exp Immunol doi: 10.1046/j.1365-2249.1998.00492.x contributor: fullname: Guo J |
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SubjectTerms | Animals Antibodies, Monoclonal - immunology autoimmune thyroid diseases CHO Cells Cricetinae Epitope Mapping Epitopes - chemistry Humans Immunodominant Epitopes - chemistry immunodominant regions Iodide Peroxidase - chemistry Iodide Peroxidase - genetics Iodide Peroxidase - immunology Iodide Peroxidase - metabolism Mice Models, Molecular mouse monoclonal antibody Mutagenesis, Site-Directed thyroid peroxidase (TPO) |
Title | Localization of key amino acid residues in the dominant conformational epitopes on thyroid peroxidase recognized by mouse monoclonal antibodies |
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