Functional Specialization of Calreticulin Domains

• Published in: The Journal of cell biology Vol. 154; no. 5; pp. 961 - 972
• Main Authors: Nakamura, Kimitoshi, Zuppini, Anna, Arnaudeau, Serge, Lynch, Jeffery, Ahsan, Irfan, Krause, Ryoko, Papp, Sylvia, De Smedt, Humbert, Parys, Jan B., Müller-Esterl, Werner, Lew, Daniel P., Krause, Karl-Heinz, Demaurex, Nicolas, Opas, Michal, Michalak, Marek
• Format: Journal Article
• Language: English
• Published: United States Rockefeller University Press 03.09.2001; The Rockefeller University Press
• Subjects: Animals; Antibodies; B lymphocytes; Biochemistry; Bradykinin - pharmacology; Bradykinin receptors; Calcium; Calcium - metabolism; Calcium Channels - genetics; Calcium Channels - metabolism; Calcium-Binding Proteins - genetics; Calcium-Binding Proteins - metabolism; Calcium-Transporting ATPases - metabolism; Calreticulin; Cell Line; Cell lines; Cell surface receptors; Cells; Cellular biology; Embryos; Endoplasmic Reticulum - metabolism; Enzyme Inhibitors - pharmacology; Fibroblasts; Fibroblasts - drug effects; Fibroblasts - physiology; Flow Cytometry; Homeostasis; Immunoblotting; Inositol 1,4,5-Trisphosphate Receptors; Mathematical functions; Mice; Mice, Knockout; Microscopy, Fluorescence; Molecular Chaperones - genetics; Molecular Chaperones - metabolism; Protein Isoforms - genetics; Protein Isoforms - metabolism; Protein Structure, Tertiary; Receptors; Receptors, Bradykinin - metabolism; Receptors, Cytoplasmic and Nuclear - genetics; Receptors, Cytoplasmic and Nuclear - metabolism; Ribonucleoproteins - genetics; Ribonucleoproteins - metabolism; Rodents; Sarcoplasmic Reticulum Calcium-Transporting ATPases; Thapsigargin - pharmacology; Transfection

Calreticulin is a Ca2+-binding chaperone in the endoplasmic reticulum (ER), and calreticulin gene knockout is embryonic lethal. Here, we used calreticulin-deficient mouse embryonic fibroblasts to examine the function of calreticulin as a regulator of Ca2+homeostasis. In cells without calreticulin, t...