Stimulation of catecholamine secretion from adrenal chromaffin cells by 14-3-3 proteins is due to reorganisation of the cortical actin network

• Published in: FEBS letters Vol. 374; no. 1; pp. 77 - 81
• Main Authors: Roth, Dagmar, Burgoyne, Robert D.
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 23.10.1995
• Subjects: 14-3-3 Protein; 14-3-3 Proteins; Actins - physiology; Adrenal Medulla - cytology; Adrenal Medulla - metabolism; Animals; Calcium; Catecholamines - metabolism; Cattle; Cell Membrane Permeability - drug effects; Cells, Cultured; Chromaffin cell; Cytochalasin D - pharmacology; Cytoskeleton - drug effects; Cytoskeleton - metabolism; Exocytosis; Phalloidine - pharmacology; Polyethylene Glycols - pharmacology; Protein Binding; Proteins - metabolism; Proteins - physiology; Secretion; Tyrosine 3-Monooxygenase; 14-3-3 Protein; Calcium; Exocytosis; Chromaffin cell; Secretion

Catecholamine release from digitonin-permeabilized adrenal chromaffin cells is increased by exogenous 14-3-3 proteins. In order to determine how 14-3-3 proteins stimulate exocytosis their effect on the cortical actin network was examined. Increased amounts of β and γ isoforms of 14-3-3 proteins were...