Enhanced antenatal detection of group B streptococcus colonization

To improve culture methods for the detection of group B streptococcus colonization. This study prospectively compared the standard culture medium, a blood agar plate, to a selective culture medium, Todd Hewitt broth with antibiotics, and compared vaginal culture with rectal culture at the first pren...

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Published inObstetrics and gynecology (New York. 1953) Vol. 85; no. 3; pp. 437 - 439
Main Authors Philipson, Elliot H., Palermino, Donato A., Robinson, Ann
Format Journal Article
LanguageEnglish
Published New York, NY Elsevier Inc 01.03.1995
The American College of Obstetricians and Gynecologists
Elsevier Science
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Abstract To improve culture methods for the detection of group B streptococcus colonization. This study prospectively compared the standard culture medium, a blood agar plate, to a selective culture medium, Todd Hewitt broth with antibiotics, and compared vaginal culture with rectal culture at the first prenatal exam. Of the 383 vaginal swabs received for evaluation of the two culture media, 78 (20.4%) were positive for group B streptococcus. The detection rates of the blood agar plate method and the Todd Hewitt broth with antibiotics were 64.1 and 97.4%, respectively. Using the Todd Hewitt broth with antibiotics, an additional 94 patients were cultured vaginaHy and rectally. Twenty-nine (30.9%) had positive cultures. The rate of detection was 58.6% for the vaginal culture, 89.7% for the rectal culture, and 100% for both culture sites combined. These data indicate that culture detection of group B streptococcus can be improved by using both a selective broth medium and a dual vaginal and rectal culture.
AbstractList To improve culture methods for the detection of group B streptococcus colonization. This study prospectively compared the standard culture medium, a blood agar plate, to a selective culture medium, Todd Hewitt broth with antibiotics, and compared vaginal culture with rectal culture at the first prenatal exam. Of the 383 vaginal swabs received for evaluation of the two culture media, 78 (20.4%) were positive for group B streptococcus. The detection rates of the blood agar plate method and the Todd Hewitt broth with antibiotics were 64.1 and 97.4%, respectively. Using the Todd Hewitt broth with antibiotics, an additional 94 patients were cultured vaginally and rectally. Twenty-nine (30.9%) had positive cultures. The rate of detection was 58.6% for the vaginal culture, 89.7% for the rectal culture, and 100% for both culture sites combined. These data indicate that culture detection of group B streptococcus can be improved by using both a selective broth medium and a dual vaginal and rectal culture.
OBJECTIVETo improve culture methods for the detection of group B streptococcus colonization.METHODSThis study prospectively compared the standard culture medium, a blood agar plate, to a selective culture medium, Todd Hewitt broth with antibiotics, and compared vaginal culture with rectal culture at the first prenatal exam.RESULTSOf the 383 vaginal swabs received for evaluation of the two culture media, 78 (20.4%) were positive for group B streptococcus. The detection rates of the blood agar plate method and the Todd Hewitt broth with antibiotics were 64.1 and 97.4%, respectively. Using the Todd Hewitt broth with antibiotics, an additional 94 patients were cultured vaginally and rectally. Twenty-nine (30.9%) had positive cultures. The rate of detection was 58.6% for the vaginal culture, 89.7% for the rectal culture, and 100% for both culture sites combined.CONCLUSIONThese data indicate that culture detection of group B streptococcus can be improved by using both a selective broth medium and a dual vaginal and rectal culture.
To improve culture methods for the detection of group B streptococcus colonization. This study prospectively compared the standard culture medium, a blood agar plate, to a selective culture medium, Todd Hewitt broth with antibiotics, and compared vaginal culture with rectal culture at the first prenatal exam. Of the 383 vaginal swabs received for evaluation of the two culture media, 78 (20.4%) were positive for group B streptococcus. The detection rates of the blood agar plate method and the Todd Hewitt broth with antibiotics were 64.1 and 97.4%, respectively. Using the Todd Hewitt broth with antibiotics, an additional 94 patients were cultured vaginaHy and rectally. Twenty-nine (30.9%) had positive cultures. The rate of detection was 58.6% for the vaginal culture, 89.7% for the rectal culture, and 100% for both culture sites combined. These data indicate that culture detection of group B streptococcus can be improved by using both a selective broth medium and a dual vaginal and rectal culture.
OBJECTIVETo improve culture methods for the detection of group B streptococcus colonization. METHODSThis study prospectively compared the standard culture medium, a blood agar plate, to a selective culture medium, Todd Hewitt broth with antibiotics, and compared vaginal culture with rectal culture at the first prenatal exam. RESULTSOf the 383 vaginal swabs received for evaluation of the two culture media, 78 (20.4%) were positive for group B streptococcus. The detection rates of the blood agar plate method and the Todd Hewitt broth with antibiotics were 64.1 and 97.4%, respectively. Using the Todd Hewitt broth with antibiotics, an additional 94 patients were cultured vaginally and rectally. Twenty-nine (30.9%) had positive cultures. The rate of detection was 58.6% for the vaginal culture, 89.7% for the rectal culture, and 100% for both culture sites combined. CONCLUSIONThese data indicate that culture detection of group B streptococcus can be improved by using both a selective broth medium and a dual vaginal and rectal culture.
Author Robinson, Ann
Philipson, Elliot H.
Palermino, Donato A.
AuthorAffiliation Department of Obstetrics and Gynecology and Pathology and Laboratory Medicine, University of Connecticut at Hartford Hospital, Hartford, Connecticut
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Human
Culture medium
Streptococcaceae
Pregnancy disorders
Streptococcus B
Bacteria
Micrococcales
Female
Bacteriological investigation
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American Academy of Pediatrics Committee on Infectious Disease, Committee on Fetus and Newborn (bib4) 1992; 90
American College of Obstetricians and Gynecologists (bib3) 1993; 37
Baker (bib5) 1977; 136
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Snippet To improve culture methods for the detection of group B streptococcus colonization. This study prospectively compared the standard culture medium, a blood agar...
OBJECTIVETo improve culture methods for the detection of group B streptococcus colonization. METHODSThis study prospectively compared the standard culture...
OBJECTIVETo improve culture methods for the detection of group B streptococcus colonization.METHODSThis study prospectively compared the standard culture...
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StartPage 437
SubjectTerms Biological and medical sciences
Carrier State - microbiology
Culture Media - economics
Diseases of mother, fetus and pregnancy
Female
Gynecology. Andrology. Obstetrics
Humans
Medical sciences
Pregnancy
Pregnancy Complications, Infectious - microbiology
Pregnancy. Fetus. Placenta
Prenatal Diagnosis - economics
Prenatal Diagnosis - methods
Prospective Studies
Rectal Diseases - microbiology
Streptococcal Infections - microbiology
Streptococcus agalactiae - isolation & purification
Vaginal Diseases - microbiology
Title Enhanced antenatal detection of group B streptococcus colonization
URI https://dx.doi.org/10.1016/0029-7844(94)00412-7
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