Oxygen Tension Regulates Survival and Fate of Mouse Central Nervous System Precursors at Multiple Levels

• Published in: Stem cells (Dayton, Ohio) Vol. 25; no. 9; pp. 2291 - 2301
• Main Authors: Chen, Hui‐Ling, Pistollato, Francesca, Hoeppner, Daniel J., Ni, Hsiao‐Tzu, McKay, Ronald D.G., Panchision, David M.
• Format: Journal Article
• Language: English
• Published: Bristol John Wiley & Sons, Ltd 01.09.2007
• Subjects: Animals; Apoptosis - drug effects; Cell Differentiation - drug effects; Cell Proliferation - drug effects; Cell Survival - drug effects; Cells, Cultured; Central Nervous System - cytology; Central Nervous System - drug effects; Central Nervous System - embryology; Expansion; Hypoxia; Hypoxia-Inducible Factor 1, alpha Subunit - metabolism; Mice; Mice, Inbred C57BL; Multipotent; Multipotent Stem Cells - cytology; Multipotent Stem Cells - drug effects; Neural stem cells; Nuclear Proteins - metabolism; Oligodendrocyte; Oxygen; Oxygen - pharmacology; Stem Cells - cytology; Stem Cells - drug effects; Tumor Suppressor Protein p53 - metabolism

Despite evidence that oxygen regulates neural precursor fate, the effects of changing oxygen tensions on distinct stages in precursor differentiation are poorly understood. We found that 5% oxygen permitted clonal and long‐term expansion of mouse fetal cortical precursors. In contrast, 20% oxygen ca...