Alteration of tumour response to radiation by interleukin-2 gene transfer
We have previously shown that BALB/c-derived EMT6 mammary tumours transfected with interleukin (IL)-2 have decreased hypoxia compared to parental tumours, due to increased vascularization. Since hypoxia is a critical factor in the response of tumours to radiation treatment, we compared the radiation...
Saved in:
Published in | British journal of cancer Vol. 82; no. 4; pp. 937 - 944 |
---|---|
Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Basingstoke
Nature Publishing Group
01.02.2000
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | We have previously shown that BALB/c-derived EMT6 mammary tumours transfected with interleukin (IL)-2 have decreased hypoxia compared to parental tumours, due to increased vascularization. Since hypoxia is a critical factor in the response of tumours to radiation treatment, we compared the radiation response of IL-2-transfected tumours to that of parental EMT6 tumours. Because the IL-2 tumours have an altered host cell composition, which could affect the interpretation of radiation sensitivity as measured by clonogenic cells, we employed flow cytometric analysis to determine the proportion of tumour cells vs host cells in each tumour type. Using this approach, we were able to correct the plating efficiency based on the number of actual tumour cells derived from tumours, making the comparison of the two types of tumours possible. We also excluded the possibility that cytotoxic T-cells present in EMT6/IL-2 tumours could influence the outcome of the clonogenic cell survival assay, by demonstrating that the plating efficiency of cells derived from EMT6/IL-2 tumours remained unchanged after depletion of Thy-1+ cells. The in vivo radiation response results demonstrated that IL-2-transfected tumours were more sensitive to radiation than parental EMT6 tumours. The hypoxic fraction of the EMT6/IL-2 tumours growing in vivo was markedly decreased relative to parental EMT6 tumours thus the increased sensitivity results from the increased vascularity we have previously observed in these tumours. These results indicate the potential therapeutic benefit of combining radiation and immunotherapy in the treatment of tumours. |
---|---|
AbstractList | We have previously shown that BALB/c-derived EMT6 mammary tumours transfected with interleukin (IL)-2 have decreased hypoxia compared to parental tumours, due to increased vascularization. Since hypoxia is a critical factor in the response of tumours to radiation treatment, we compared the radiation response of IL-2-transfected tumours to that of parental EMT6 tumours. Because the IL-2 tumours have an altered host cell composition, which could affect the interpretation of radiation sensitivity as measured by clonogenic cells, we employed flow cytometric analysis to determine the proportion of tumour cells vs host cells in each tumour type. Using this approach, we were able to correct the plating efficiency based on the number of actual tumour cells derived from tumours, making the comparison of the two types of tumours possible. We also excluded the possibility that cytotoxic T-cells present in EMT6/IL-2 tumours could influence the outcome of the clonogenic cell survival assay, by demonstrating that the plating efficiency of cells derived from EMT6/IL-2 tumours remained unchanged after depletion of Thy-1+ cells. The in vivo radiation response results demonstrated that IL-2-transfected tumours were more sensitive to radiation than parental EMT6 tumours. The hypoxic fraction of the EMT6/IL-2 tumours growing in vivo was markedly decreased relative to parental EMT6 tumours thus the increased sensitivity results from the increased vascularity we have previously observed in these tumours. These results indicate the potential therapeutic benefit of combining radiation and immunotherapy in the treatment of tumours. We have previously shown that BALB/c-derived EMT6 mammary tumours transfected with interleukin (IL)-2 have decreased hypoxia compared to parental tumours, due to increased vascularization. Since hypoxia is a critical factor in the response of tumours to radiation treatment, we compared the radiation response of IL-2-transfected tumours to that of parental EMT6 tumours. Because the IL-2 tumours have an altered host cell composition, which could affect the interpretation of radiation sensitivity as measured by clonogenic cells, we employed flow cytometric analysis to determine the proportion of tumour cells vs host cells in each tumour type. Using this approach, we were able to correct the plating efficiency based on the number of actual tumour cells derived from tumours, making the comparison of the two types of tumours possible. We also excluded the possibility that cytotoxic T-cells present in EMT6/IL-2 tumours could influence the outcome of the clonogenic cell survival assay, by demonstrating that the plating efficiency of cells derived from EMT6/IL-2 tumours remained unchanged after depletion of Thy-1 + cells. The in vivo radiation response results demonstrated that IL-2-transfected tumours were more sensitive to radiation than parental EMT6 tumours. The hypoxic fraction of the EMT6/IL-2 tumours growing in vivo was markedly decreased relative to parental EMT6 tumours thus the increased sensitivity results from the increased vascularity we have previously observed in these tumours. These results indicate the potential therapeutic benefit of combining radiation and immunotherapy in the treatment of tumours. © 2000 Cancer Research Campaign We have previously shown that BALB/c-derived EMT6 mammary tumours transfected with interleukin (IL)-2 have decreased hypoxia compared to parental tumours, due to increased vascularization. Since hypoxia is a critical factor in the response of tumours to radiation treatment, we compared the radiation response of IL-2-transfected tumours to that of parental EMT6 tumours. Because the IL-2 tumours have an altered host cell composition, which could affect the interpretation of radiation sensitivity as measured by clonogenic cells, we employed flow cytometric analysis to determine the proportion of tumour cells vs host cells in each tumour type. Using this approach, we were able to correct the plating efficiency based on the number of actual tumour cells derived from tumours, making the comparison of the two types of tumours possible. We also excluded the possibility that cytotoxic T-cells present in EMT6/IL-2 tumours could influence the outcome of the clonogenic cell survival assay, by demonstrating that the plating efficiency of cells derived from EMT6/IL-2 tumours remained unchanged after depletion of Thy-1 super(+)cells. The in vivo radiation response results demonstrated that IL-2-transfected tumours were more sensitive to radiation than parental EMT6 tumours. The hypoxic fraction of the EMT6/IL-2 tumours growing in vivo was markedly decreased relative to parental EMT6 tumours thus the increased sensitivity results from the increased vascularity we have previously observed in these tumours. These results indicate the potential therapeutic benefit of combining radiation and immunotherapy in the treatment of tumours. [copy 2000 Cancer Research Campaign |
Author | KENG, P. C LORD, E. M LEE, J FENTON, B. M MORAN, J. P KOCH, C. J FRELINGER, J. G |
Author_xml | – sequence: 1 givenname: J surname: LEE fullname: LEE, J organization: Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, United States – sequence: 2 givenname: J. P surname: MORAN fullname: MORAN, J. P organization: Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, United States – sequence: 3 givenname: B. M surname: FENTON fullname: FENTON, B. M organization: Cancer Center Immunology Program, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, United States – sequence: 4 givenname: C. J surname: KOCH fullname: KOCH, C. J organization: Department of Radiation Oncology, University of Pennsylvania, Philadelphia, PA 19104, United States – sequence: 5 givenname: J. G surname: FRELINGER fullname: FRELINGER, J. G organization: Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, United States – sequence: 6 givenname: P. C surname: KENG fullname: KENG, P. C organization: Department of Radiation Oncology, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, United States – sequence: 7 givenname: E. M surname: LORD fullname: LORD, E. M organization: Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, United States |
BackLink | http://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1326568$$DView record in Pascal Francis https://www.ncbi.nlm.nih.gov/pubmed/10732769$$D View this record in MEDLINE/PubMed |
BookMark | eNp9kc1v1DAQxS1URLeFKzdQhBCcsvgjsTMXpKoCWqkSFzhbjndcvGTtxU4q9b_HaVal9NCTNXq_eZ6Zd0KOQgxIyGtG14y2zad-G-2aAUApOX9GVqwVvGYdV0dkRSlVNQVOj8lJzttSAu3UC3LMqBJcSViRy7NhxGRGH0MVXTVOuzilKmHex5CxGmOVzMYven9b-VDoAaffPtS8usZQkGRCdphekufODBlfHd5T8vPrlx_nF_XV92-X52dXtW1AjjU6yY0A0zvLqXJGut4oaRl2VEArHQrXABV0I7peYOuMwJ4xhoIxMFx04pR8Xnz3U7_DjcVQJhj0PvmdSbc6Gq__V4L_pa_jjeZCNQ2dDT4eDFL8M2Ee9c5ni8NgAsYp6w6ACSaUKuSHJ0lFoaNSNQV89wjcljOGcgbNOUAnJMxu6wWyKeac0N3PzKiew9RzmHoOU89hloa3Dzd9gC_pFeD9ATDZmsGVJKzP_zjBZSvnhd8sWDDjlPBelxLu_vkLRKGz9Q |
CODEN | BJCAAI |
CitedBy_id | crossref_primary_10_1016_j_ghir_2015_02_001 crossref_primary_10_1269_jrr_10185 crossref_primary_10_1016_j_celrep_2016_02_033 crossref_primary_10_1177_039463201102400324 crossref_primary_10_1016_j_ghir_2012_07_003 crossref_primary_10_1016_j_asr_2006_11_010 crossref_primary_10_4049_immunohorizons_1900017 crossref_primary_10_1186_1476_5926_5_6 crossref_primary_10_1667_RR3196 crossref_primary_10_1006_excr_2000_5121 crossref_primary_10_1007_s11481_012_9423_7 crossref_primary_10_1186_1479_5876_10_154 crossref_primary_10_1016_j_yexmp_2012_09_023 crossref_primary_10_1158_0008_5472_CAN_12_0210 crossref_primary_10_3390_ijms23031653 crossref_primary_10_1016_j_radonc_2010_03_009 crossref_primary_10_1007_s10911_010_9194_9 crossref_primary_10_1152_physiolgenomics_90372_2008 crossref_primary_10_2217_imt_11_155 crossref_primary_10_1586_17446651_1_1_33 crossref_primary_10_1152_ajpheart_01330_2006 crossref_primary_10_1152_ajpheart_00885_2002 crossref_primary_10_1016_S1535_6108_03_00240_X crossref_primary_10_1038_cgt_2012_95 crossref_primary_10_3389_fonc_2024_1265762 crossref_primary_10_1016_j_ijrobp_2004_01_030 crossref_primary_10_1016_j_arcmed_2007_12_005 crossref_primary_10_1016_j_ymthe_2005_03_027 crossref_primary_10_1039_b911313b crossref_primary_10_1016_j_molbrainres_2005_02_011 crossref_primary_10_1111_j_0022_202X_2004_22735_x |
ContentType | Journal Article |
Copyright | 2000 INIST-CNRS Copyright Nature Publishing Group Feb 2000 Copyright © 2000 Cancer Research Campaign 2000 Cancer Research Campaign |
Copyright_xml | – notice: 2000 INIST-CNRS – notice: Copyright Nature Publishing Group Feb 2000 – notice: Copyright © 2000 Cancer Research Campaign 2000 Cancer Research Campaign |
DBID | IQODW CGR CUY CVF ECM EIF NPM AAYXX CITATION 3V. 7RV 7TO 7U9 7X7 7XB 88E 8AO 8C1 8FE 8FH 8FI 8FJ 8FK ABUWG AFKRA AN0 AZQEC BBNVY BENPR BHPHI CCPQU DWQXO FYUFA GHDGH GNUQQ H94 HCIFZ K9. KB0 LK8 M0S M1P M7P NAPCQ PQEST PQQKQ PQUKI PRINS 7X8 8FD FR3 P64 RC3 5PM |
DOI | 10.1054/bjoc.1999.1022 |
DatabaseName | Pascal-Francis Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed CrossRef ProQuest Central (Corporate) ProQuest Nursing and Allied Health Journals Oncogenes and Growth Factors Abstracts Virology and AIDS Abstracts ProQuest_Health & Medical Collection ProQuest Central (purchase pre-March 2016) Medical Database (Alumni Edition) ProQuest Pharma Collection ProQuest Public Health Database ProQuest SciTech Collection ProQuest Natural Science Collection Hospital Premium Collection Hospital Premium Collection (Alumni Edition) ProQuest Central (Alumni) (purchase pre-March 2016) ProQuest Central (Alumni) ProQuest Central British Nursing Database ProQuest Central Essentials Biological Science Collection ProQuest Central ProQuest Natural Science Collection ProQuest One Community College ProQuest Central Korea Health Research Premium Collection Health Research Premium Collection (Alumni) ProQuest Central Student AIDS and Cancer Research Abstracts SciTech Premium Collection (Proquest) (PQ_SDU_P3) ProQuest Health & Medical Complete (Alumni) Nursing & Allied Health Database (Alumni Edition) Biological Sciences Health & Medical Collection (Alumni Edition) PML(ProQuest Medical Library) Biological Science Database Nursing & Allied Health Premium ProQuest One Academic Eastern Edition (DO NOT USE) ProQuest One Academic ProQuest One Academic UKI Edition ProQuest Central China MEDLINE - Academic Technology Research Database Engineering Research Database Biotechnology and BioEngineering Abstracts Genetics Abstracts PubMed Central (Full Participant titles) |
DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) CrossRef ProQuest Central Student Oncogenes and Growth Factors Abstracts ProQuest Central Essentials ProQuest Health & Medical Complete (Alumni) ProQuest Central (Alumni Edition) SciTech Premium Collection ProQuest One Community College ProQuest Natural Science Collection ProQuest Pharma Collection ProQuest Central China ProQuest Central Health Research Premium Collection Health and Medicine Complete (Alumni Edition) Natural Science Collection ProQuest Central Korea Biological Science Collection AIDS and Cancer Research Abstracts ProQuest Medical Library (Alumni) ProQuest Public Health Virology and AIDS Abstracts ProQuest Biological Science Collection ProQuest One Academic Eastern Edition British Nursing Index with Full Text ProQuest Nursing & Allied Health Source ProQuest Hospital Collection Health Research Premium Collection (Alumni) Biological Science Database ProQuest SciTech Collection ProQuest Hospital Collection (Alumni) Nursing & Allied Health Premium ProQuest Health & Medical Complete ProQuest Medical Library ProQuest One Academic UKI Edition ProQuest Nursing & Allied Health Source (Alumni) ProQuest One Academic ProQuest Central (Alumni) MEDLINE - Academic Genetics Abstracts Engineering Research Database Technology Research Database Biotechnology and BioEngineering Abstracts |
DatabaseTitleList | ProQuest Central Student MEDLINE - Academic MEDLINE Genetics Abstracts |
Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database – sequence: 3 dbid: BENPR name: ProQuest Central url: https://www.proquest.com/central sourceTypes: Aggregation Database |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Medicine Dentistry |
EISSN | 1532-1827 |
EndPage | 944 |
ExternalDocumentID | 1002351881 10_1054_bjoc_1999_1022 10732769 1326568 6691022 |
Genre | Research Support, U.S. Gov't, P.H.S Journal Article |
GeographicLocations | United States--US |
GeographicLocations_xml | – name: United States--US |
GrantInformation_xml | – fundername: NCI NIH HHS grantid: CA52586 – fundername: NCI NIH HHS grantid: CA28332 – fundername: NCI NIH HHS grantid: CA74071 |
GroupedDBID | - -Q- 02 08R 0R 23N 39C 3V. 4.4 53G 55 5GY 5RE 70F 7RV 7X7 88E 8AO 8C1 8FI 8FJ 8R4 8R5 8WZ A6W AADWK AAPBV AAWBL AAWTL AAYJO AAZLF ABAWZ ABDBF ABDEU ABFLS ABGIJ ABOCM ABPTK ABUWG ACGFS ACKTT ACPRK ADACO ADBBV ADBIT ADHDB ADQMX AEDAW AEJRE AENEX AEXYK AFKRA AFRAH AFSHS AGEZK AHMBA AHSBF AJRNO ALFFA ALMA_UNASSIGNED_HOLDINGS AN0 ASPBG AVWKF AXYYD AZFZN B0M BAWUL BBAFP BBNVY BENPR BHPHI BKEYQ BKKNO BNQBC BPHCQ BVXVI CAG COF CS3 DIK DU5 E3Z EAD EAP EAS EBC EBD EBLON EBS EE. EIOEI EJD EMB EMK EPL ESX EX3 F5P FDQFY FEDTE FERAY FIZPM FRJ FSGXE FYUFA GJ GX1 HCIFZ HVGLF HYE HZ IH2 J5H JSO KQ8 M1P M41 M7P NAO NAPCQ O9- OK1 P2P PQEST PQQKQ PQUKI PRINS PROAC PSQYO Q- Q2X RIG RNT RNTTT RPM SNX SNYQT SV3 SWTZT TAOOD TBHMF TDRGL TR2 TUS UDS VH1 W2D WH7 WOW X7M XFK Y6R ZA5 ZGI --- .55 .GJ 0R~ 36B 406 6J9 AANZL AATNV AAUGY AAYFA ABLJU ACBMV ACBRV ACBYP ACGFO ACIGE ACRQY ACTTH ACVWB ADFRT ADMDM ADYYL AEFTE AEVLU AFNRJ AGAYW AGGBP AGHAI AI. AILAN AJCLW AJDOV AMRJV AMYLF AOIJS CCPQU DNIVK DPUIP EMOBN HZ~ IQODW IWAJR JZLTJ NQJWS NYICJ SOHCF SRMVM UKHRP ~02 ~8M AACDK AASML ABAKF ABZZP ACAOD ACZOJ AEFQL AEMSY AFBBN AGQEE AIGIU CGR CUY CVF ECM EIF FIGPU HMCUK NPM AAYXX CITATION 7TO 7U9 7XB 8FE 8FH 8FK AZQEC DWQXO GNUQQ H94 K9. LK8 7X8 8FD FR3 P64 RC3 5PM |
ID | FETCH-LOGICAL-c496t-ef62a39abfc207fa6fba76c1e803956fe3f49030d38b3e5fa3eb111e3119a2383 |
IEDL.DBID | RPM |
ISSN | 0007-0920 |
IngestDate | Tue Sep 17 21:13:19 EDT 2024 Fri Aug 16 00:02:12 EDT 2024 Fri Aug 16 04:53:57 EDT 2024 Fri Sep 13 00:37:01 EDT 2024 Fri Aug 23 04:03:54 EDT 2024 Sat Sep 28 07:35:46 EDT 2024 Sun Oct 29 17:09:35 EDT 2023 Thu Oct 07 19:36:11 EDT 2021 |
IsDoiOpenAccess | true |
IsOpenAccess | true |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 4 |
Keywords | Antineoplastic agent Animal model Carcinoma Radiosensitivity Cytokine Rodentia Malignant tumor Radiotherapy Genetic transfer Interleukin 6 Mammary gland diseases Vertebrata Mammalia Transfection Mouse Animal Immunotherapy Established cell line Genetic engineering Combined treatment Mammary gland |
Language | English |
License | CC BY 4.0 This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/. |
LinkModel | DirectLink |
MergedId | FETCHMERGED-LOGICAL-c496t-ef62a39abfc207fa6fba76c1e803956fe3f49030d38b3e5fa3eb111e3119a2383 |
Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ObjectType-Article-2 ObjectType-Feature-1 |
OpenAccessLink | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2374408/ |
PMID | 10732769 |
PQID | 229983697 |
PQPubID | 41855 |
PageCount | 8 |
ParticipantIDs | pubmedcentral_primary_oai_pubmedcentral_nih_gov_2374408 proquest_miscellaneous_899131377 proquest_miscellaneous_70980674 proquest_journals_229983697 crossref_primary_10_1054_bjoc_1999_1022 pubmed_primary_10732769 pascalfrancis_primary_1326568 nature_primary_6691022 |
ProviderPackageCode | ABDEU AEDAW AAZLF -Q- AADWK AAYJO 70F ADQMX EE. RNTTT SWTZT ABGIJ RNT NAO |
PublicationCentury | 2000 |
PublicationDate | 2000-02-01 |
PublicationDateYYYYMMDD | 2000-02-01 |
PublicationDate_xml | – month: 02 year: 2000 text: 2000-02-01 day: 01 |
PublicationDecade | 2000 |
PublicationPlace | Basingstoke |
PublicationPlace_xml | – name: Basingstoke – name: England – name: London |
PublicationTitle | British journal of cancer |
PublicationTitleAlternate | Br J Cancer |
PublicationYear | 2000 |
Publisher | Nature Publishing Group |
Publisher_xml | – name: Nature Publishing Group |
References | Hill, RP 1986; 12 Lee, J, Siemann, DW, Koch, CJ, Lord, EM 1996; 67 Gansbacher, B, Zier, K, Daniels, B, Cronin, K, Bannerji, R, Gilboa, E 1990; 172 Ledbetter, JA, Herzenberg, LA 1979; 47 Evans, SM, Jenkins, WT, Joiner, B, Lord, EM, Koch, CJ 1996; 56 McAdam, AJ, Pulaski, BA, Storozynsky, E, Yeh, KY, Sickel, JZ, Frelinger, JG, Lord, EM 1995; 165 Badalament, RA, Cibas, ES, Reuter, VE, Fair, WR, Melamed, MR 1987; 137 Colombo, MP, Ferrari, G, Stoppacciaro, A, Parenza, M, Rodolfo, M, Mavilio, F, Parmiani, G 1991; 173 Mor, F, Cohen, IR 1996; 156 Chang, AY, Keng, PC 1987; 47 Chiang, CS, Syljuasen, RG, Hong, JH, Wallis, A, Dougherty, GJ, McBride, WH 1997; 57 James, SE, Arlett, CF, Green, MH, Bridges, BA 1983; 44 Cameron, RB, Spiess, PJ, Rosenberg, SA 1990; 171 Yokota, T, Arai, N, Lee, F, Rennick, D, Mosmann, T, Arai, K 1985; 82 Hietanen, T, Kellokumpu-Lehtinen, P, Pitkanen, M 1995; 67 McBride, WH, Economou, JS, Kuber, N, Hong, JH, Chiang, CS, Syljuasen, R, Dougherty, ST, Dougherty, GJ 1995; 34 Kwok, TT, Sutherland, RM 1992; 22 Chadwick, KH, Leenhouts, HP 1973; 18 Seki, H, Iwai, K, Kanegane, H, Konno, A, Ohta, K, Ohta, K, Yachie, A, Taniguchi, N, Miyawaki, T 1995; 163 McAdam, AJ, Felcher, A, Woods, ML, Pulaski, BA, Hutter, EK, Frelinger, JG, Lord, EM 1994; 15 Gerber, M, Guichard, M, Pioch, Y, Dubois, JB 1989; 120 Hockel, M, Knoop, C, Schlenger, K, Vorndran, B, Baussmann, E, Mitze, M, Knapstein, PG, Vaupel, P 1993; 26 Koch, CJ, Evans, SM, Lord, EM 1995; 72 Pulaski, BA, Yeh, KY, Shastri, N, Maltby, KM, Penney, DP, Lord, EM, Frelinger, JG 1996; 93 Syljuasen, RG, Belldegrun, A, Tso, CL, Withers, HR, McBride, WH 1997; 148 Jaffee, EM, Pardoll, DM 1997; 12 Miller, AR, McBride, WH, Hunt, K, Economou, JS 1994; 1 Moulder, JE, Rockwell, S 1984; 10 Gatenby, RA, Kessler, HB, Rosenblum, JS, Coia, LR, Moldofsky, PJ, Hartz, WH, Broder, GJ 1988; 14 Fearon, ER, Pardoll, DM, Itaya, T, Golumbek, P, Levitsky, HI, Simons, JW, Karasuyama, H, Vogelstein, B, Frost, P 1990; 60 Lee, J, Fenton, BM, Koch, CJ, Frelinger, JG, Lord, EM 1998; 58 Braunschweiger, PG, Basrur, V, Santos, O, Adessa, A, Houdek, P, Markoe, AM 1996; 145 Gunning, P, Leavitt, J, Muscat, G, Ng, SY, Kedes, L 1987; 84 Howell, RL, Koch, CJ 1980; 6 1735690 - Int J Radiat Oncol Biol Phys. 1992;22(3):525-7 2212951 - J Exp Med. 1990 Oct 1;172(4):1217-24 398327 - Immunol Rev. 1979;47:63-90 6605945 - Int J Radiat Biol Relat Stud Phys Chem Med. 1983 Nov;44(5):417-22 2440031 - Proc Natl Acad Sci U S A. 1987 Jul;84(14):4831-5 8543801 - J Immunol. 1996 Jan 15;156(2):515-22 7533816 - Int J Radiat Biol. 1995 Feb;67(2):119-26 8542599 - Cancer Res. 1996 Jan 15;56(2):405-11 8707411 - Int J Cancer. 1996 Jul 29;67(3):372-8 9355869 - Radiat Res. 1997 Nov;148(5):443-8 4803965 - Phys Med Biol. 1973 Jan;18(1):78-87 2440565 - Cancer Res. 1987 Aug 15;47(16):4338-41 3360652 - Int J Radiat Oncol Biol Phys. 1988 May;14(5):831-8 2137372 - Cell. 1990 Feb 9;60(3):397-403 8438086 - Radiother Oncol. 1993 Jan;26(1):45-50 9307268 - Cancer Res. 1997 Sep 15;57(18):3899-903 8032538 - J Immunother Emphasis Tumor Immunol. 1994 Apr;15(3):155-64 2943705 - Int J Radiat Oncol Biol Phys. 1986 Jul;12(7):1049-54 7779438 - Acta Oncol. 1995;34(3):447-51 7758128 - Cell Immunol. 1995 Jun;163(1):30-6 7547233 - Br J Cancer. 1995 Oct;72(4):869-74 3586147 - J Urol. 1987 Jun;137(6):1159-62 6735758 - Int J Radiat Oncol Biol Phys. 1984 May;10(5):695-712 2295878 - J Exp Med. 1990 Jan 1;171(1):249-63 9537251 - Cancer Res. 1998 Apr 1;58(7):1478-85 7850546 - Ann Surg Oncol. 1994 Sep;1(5):436-50 2798779 - Radiat Res. 1989 Oct;120(1):164-76 7390904 - Int J Radiat Oncol Biol Phys. 1980 Mar;6(3):311-8 8622994 - Proc Natl Acad Sci U S A. 1996 Apr 16;93(8):3669-74 1706752 - J Exp Med. 1991 Apr 1;173(4):889-97 7553882 - Cell Immunol. 1995 Oct 15;165(2):183-92 3918306 - Proc Natl Acad Sci U S A. 1985 Jan;82(1):68-72 8606924 - Radiat Res. 1996 Feb;145(2):150-6 9184378 - Methods. 1997 Jun;12(2):143-53 |
References_xml | – volume: 172 start-page: 1217 year: 1990 end-page: 1224 article-title: Interleukin 2 gene transfer into tumor cells abrogates tumorigenicity and induces protective immunity publication-title: J Exp Med contributor: fullname: Gilboa, E – volume: 58 start-page: 1478 year: 1998 end-page: 1485 article-title: Interleukin 2 expression by tumor cells alters both the immune response and the tumor microenvironment publication-title: Cancer Res contributor: fullname: Lord, EM – volume: 56 start-page: 405 year: 1996 end-page: 411 article-title: 2-Nitroimidazole (EF5) binding predicts radiation resistance in individual 9L s.c. tumors publication-title: Cancer Res contributor: fullname: Koch, CJ – volume: 60 start-page: 397 year: 1990 end-page: 403 article-title: Interleukin-2 production by tumor cells bypasses T helper function in the generation of an antitumor response publication-title: Cell contributor: fullname: Frost, P – volume: 67 start-page: 119 year: 1995 end-page: 126 article-title: Action of recombinant interferons and interleukin 2 in modulating radiation effects on viability and cytotoxicity of large granular lymphocytes publication-title: Int J Radiat Biol contributor: fullname: Pitkanen, M – volume: 15 start-page: 155 year: 1994 end-page: 164 article-title: Transfection of transforming growth factor-beta producing tumor EMT6 with interleukin-2 elicits tumor rejection and tumor reactive cytotoxic T-lymphocytes publication-title: J Immunother Emphasis Tumor Immunol contributor: fullname: Lord, EM – volume: 148 start-page: 443 year: 1997 end-page: 448 article-title: Sensitization of renal carcinoma to radiation using alpha interferon (IFNA) gene transfection publication-title: Radiat Res contributor: fullname: McBride, WH – volume: 26 start-page: 45 year: 1993 end-page: 50 article-title: Intratumoral pO predicts survival in advanced cancer of the uterine cervix publication-title: Radiother Oncol contributor: fullname: Vaupel, P – volume: 22 start-page: 525 year: 1992 end-page: 527 article-title: Cell cycle dependence of epidermal growth factor induced radiosensitization publication-title: Int J Radiat Oncol Biol Physics contributor: fullname: Sutherland, RM – volume: 12 start-page: 1049 year: 1986 end-page: 1054 article-title: Sensitizers and radiation dose fractionation: results and interpretations publication-title: Int J Radiat Oncol Biol Physics contributor: fullname: Hill, RP – volume: 12 start-page: 143 year: 1997 end-page: 153 article-title: Considerations for the clinical development of cytokine gene-transduced tumor cell vaccines publication-title: Methods contributor: fullname: Pardoll, DM – volume: 10 start-page: 695 year: 1984 end-page: 712 article-title: Hypoxic fractions of solid tumors: experimental techniques, methods of analysis, and a survey of existing data publication-title: Int J Radiat Oncol Biol Physics contributor: fullname: Rockwell, S – volume: 93 start-page: 3669 year: 1996 end-page: 3674 article-title: Interleukin 3 enhances cytotoxic T lymphocyte development and class I major histocompatibility complex ‘re-presentation’ of exogenous antigen by tumor-infiltrating antigen-presenting cells publication-title: Proc Natl Acad Sci USA contributor: fullname: Frelinger, JG – volume: 171 start-page: 249 year: 1990 end-page: 263 article-title: Synergistic antitumor activity of tumor-infiltrating lymphocytes, interleukin 2, and local tumor irradiation. Studies on the mechanism of action publication-title: J Exp Med contributor: fullname: Rosenberg, SA – volume: 137 start-page: 1159 year: 1987 end-page: 1162 article-title: Flow cytometric analysis of primary adenocarcinoma of the bladder publication-title: J Urol contributor: fullname: Melamed, MR – volume: 165 start-page: 183 year: 1995 end-page: 192 article-title: Analysis of the effect of cytokines (interleukins 2, 3, 4, and 6, granulocyte-monocyte colony-stimulating factor, and interferon-gamma) on generation of primary cytotoxic T lymphocytes against a weakly immunogenic tumor publication-title: Cell Immunol contributor: fullname: Lord, EM – volume: 84 start-page: 4831 year: 1987 end-page: 4835 article-title: A human beta-actin expression vector system directs high-level accumulation of antisense transcripts publication-title: Proc Natl Acad Sci USA contributor: fullname: Kedes, L – volume: 67 start-page: 372 year: 1996 end-page: 378 article-title: Direct relationship between radiobiological hypoxia in tumors and monoclonal antibody detection of EF5 cellular adducts publication-title: Int J Cancer contributor: fullname: Lord, EM – volume: 72 start-page: 869 year: 1995 end-page: 874 article-title: Oxygen dependence of cellular uptake of EF5 [2-(2-nitro-1H-imidazol-1-yl)-N-(2,2,3,3,3-pentafluoropropyl)acetamide]: analysis of drug adducts by fluroescent antibodies vs bound radioactivity publication-title: Br J Cancer contributor: fullname: Lord, EM – volume: 57 start-page: 3899 year: 1997 end-page: 3903 article-title: Effects of IL-3 gene expression on tumor response to irradiation in vitro and publication-title: Cancer Res contributor: fullname: McBride, WH – volume: 18 start-page: 78 year: 1973 end-page: 87 article-title: A molecular theory of cell survival publication-title: Physics Med Biol contributor: fullname: Leenhouts, HP – volume: 163 start-page: 30 year: 1995 end-page: 36 article-title: Differential protective action of cytokines on radiation-induced apoptosis of peripheral lymphocyte subpopulations publication-title: Cell Immunol contributor: fullname: Miyawaki, T – volume: 34 start-page: 447 year: 1995 end-page: 451 article-title: Modification of tumor microenvironment by cytokine gene transfer publication-title: Acta Oncol contributor: fullname: Dougherty, GJ – volume: 120 start-page: 164 year: 1989 end-page: 176 article-title: The influence of interleukin-2, feeder cells, and timing of irradiation on the radiosensitivity of human T lymphocytes assessed by the colony-forming assay publication-title: Radiat Res contributor: fullname: Dubois, JB – volume: 44 start-page: 417 year: 1983 end-page: 422 article-title: Radiosensitivity of human T-lymphocytes proliferating in long term culture publication-title: Int J Radiat Biol Related Studies Physics, Chem Med contributor: fullname: Bridges, BA – volume: 173 start-page: 889 year: 1991 end-page: 897 article-title: Granulocyte colony-stimulating factor gene transfer suppresses tumorigenicity of a murine adenocarcinoma publication-title: J Exp Med contributor: fullname: Parmiani, G – volume: 47 start-page: 63 year: 1979 end-page: 90 article-title: Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens publication-title: Immunol Rev contributor: fullname: Herzenberg, LA – volume: 82 start-page: 68 year: 1985 end-page: 72 article-title: Use of a cDNA expression vector for isolation of mouse interleukin 2 cDNA clones: expression of T-cell growth-factor activity after transfection of monkey cells publication-title: Proc Natl Acad Sci USA contributor: fullname: Arai, K – volume: 14 start-page: 831 year: 1988 end-page: 838 article-title: Oxygen distribution in squamous cell carcinoma metastases and its relationship to outcome of radiation therapy publication-title: Int J Radiat Oncol Biol Physics contributor: fullname: Broder, GJ – volume: 6 start-page: 311 year: 1980 end-page: 318 article-title: The disaggregation, separation and identification of cells from irradiated and unirradiated EMT6 mouse tumors publication-title: Int J Radiat Oncol Biol Physics contributor: fullname: Koch, CJ – volume: 47 start-page: 4338 year: 1987 end-page: 4341 article-title: Potentiation of radiation cytotoxicity by recombinant interferons, a phenomenon associated with increased blockage at the G2-M phase of the cell cycle publication-title: Cancer Res contributor: fullname: Keng, PC – volume: 1 start-page: 436 year: 1994 end-page: 450 article-title: Cytokine-mediated gene therapy for cancer publication-title: Ann Surg Oncol contributor: fullname: Economou, JS – volume: 145 start-page: 150 year: 1996 end-page: 156 article-title: Radioresistance in murine solid tumors induced by interleukin-1 publication-title: Radiat Res contributor: fullname: Markoe, AM – volume: 156 start-page: 515 year: 1996 end-page: 522 article-title: IL-2 rescues antigen-specific T cells from radiation or dexamethasone-induced apoptosis. Correlation with induction of Bcl-2 publication-title: J Immunol contributor: fullname: Cohen, IR |
SSID | ssj0009087 |
Score | 1.7409061 |
Snippet | We have previously shown that BALB/c-derived EMT6 mammary tumours transfected with interleukin (IL)-2 have decreased hypoxia compared to parental tumours, due... |
SourceID | pubmedcentral proquest crossref pubmed pascalfrancis nature |
SourceType | Open Access Repository Aggregation Database Index Database Publisher |
StartPage | 937 |
SubjectTerms | Animals Antineoplastic agents Biological and medical sciences Cancer research Cancer therapies Combined treatments (chemotherapy of immunotherapy associated with an other treatment) Cytokines Cytotoxicity Dentistry Gene Transfer Techniques Hypoxia Immune system Immunology Immunotherapy Interleukin-2 - genetics Laboratories Medical research Medical sciences Mice Mice, Inbred BALB C Oncology Pharmacology. Drug treatments Radiation therapy Radiation Tolerance - genetics Regular T-Lymphocytes, Cytotoxic - immunology Tumors |
SummonAdditionalLinks | – databaseName: ProQuest_Health & Medical Collection dbid: 7X7 link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1Lb9QwEB6VIgESQlAoDaXgAxKnaNd24scJVUApSOVEpb1FdtYWLSXZZrMH_j0zebWLgPNMomRmPA97_A3AG-VssMvoUkuVTqYdTzGJLVPvguFKOx853R0--6pOz7Mvi3yxA5_GuzDUVjn6xM5RL-uS9shnAv2mkcrqmfO0CVC2s3er65TGR9Ex6zBL4w7c5QKzCjRsvdA36Ltz04Nn0r6cFfMRvTHPZv6yLunKniUQA7EVnSZszYcrt0aJxX7Uxd9y0T9bKm_FqJPH8GhILtlxbw1PYCdUe3D_AzUE0Uy3Pbh3NhylP4XPx1cdoDLqhdWRtZuf-DRr-pbZwNqaNQRb0NH9L0awEs1V2Py4qFLB0OqQpct5Q_MMzk8-fnt_mg5zFdIys6pNQ1TCSYt6KMVcR6eid1qVPJi5xHIpBhkzi4t_KY2XIY9OokPnPEjOrcMQL_dht6qrcAAsD87kpc-kCDKLUVq_RC8QhHHRYW3EE3g7yrNY9fAZRXfsnWcFSb4gyRck-QT2e3FPfErZnnC0Jf6b92DmmSuTwOGojmJYf-tispYEXk9UXDh0GuKqUG_WhZ5bg6E6S4D9gwNLUS4JkTGB5712b_2DlkIrm4De0vvEQKjd25Tq4nuH3i0kYTKaF__97EN40N_5p76Zl7DbNptwhNlP6191lv0bdsgGEw priority: 102 providerName: ProQuest |
Title | Alteration of tumour response to radiation by interleukin-2 gene transfer |
URI | http://dx.doi.org/10.1054/bjoc.1999.1022 https://www.ncbi.nlm.nih.gov/pubmed/10732769 https://www.proquest.com/docview/229983697/abstract/ https://search.proquest.com/docview/70980674 https://search.proquest.com/docview/899131377 https://pubmed.ncbi.nlm.nih.gov/PMC2374408 |
Volume | 82 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Lb9NAEB61RUJICPEquKVhD0ic3Hi99j6OJWpVkFJViEq5WbvOrgikduQ4B_59Z712SBBcuOQya8v5ZsYz4535FuAD18qqudOx8pVOJjSNMYktY6OtpFxo46ifHZ7e8Ou77Mssnx1APszCdE37pVmcV8v782rxveutXN2X46FPbHw7naTM09rJ8SEcCsaGEn1g2k1kIMr03-BUmgxMjXk2Nj_q0o_nKU9Y0J1jgwaeCt_svBOUtpSaT1d6jUC5cMLF31LQPzspd0LT1XN41ueU5CI8-ws4sNVLeDztd81fweeLZcedjCogtSPtBsv9hjShO9aStiaNZyjo5OYX8QwSzdJufi6qOCVoYLikS29t8xruri6_Ta7j_giFuMwUb2PreKqZQsjLNBFOc2e04CW1MmFYGTnLXKbQz-dMGmZzpxm-uym1jFKlMZqzYziq6sq-BZJbLfPSZCy1LHOOKTNHh7ep1E5jGUQj-DhgWKwCU0bR7XDnWeGBLzzwhQc-guMA8XYd5yoIzvYg_30fTDJzLiM4HVRQ9K62LlIMqJJxJSJ4v5Wij_iND13ZerMuRKIkRuUsAvKPFVh1UubJFyN4EzS68x-CjUQg9nS9XeAJuvclaLcdUXdvpyf_feUpPAmj_7595h0ctc3GnmES1JoRmv5M4K-c0BE8-nR5c_t11DnCA07_CwE |
link.rule.ids | 230,315,733,786,790,891,12083,12250,21416,27957,27958,31754,31755,33301,33302,33779,33780,43345,43614,43840,53827,53829,74102,74371,74659 |
linkProvider | National Library of Medicine |
linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1Lb9RADLagSBQJVVAopA86ByROUTOPTGZOqCpUW-j21Eq9RZPsjFqokiWbPfDvsZNs2kXQs60osWf8iO3PAB-1s97OgostZToqczzGILaMC-cN15krAqfZ4emFnlypb9fp9dCbsxjaKlc2sTPUs7qkf-RHAu2mkdpmn-e_YloaRcXVYYPGU3imJPoZGhQ_ue_wsInpITPpb5wVyQqzMVVHxY-6pEE9S9AFYs0njYiaL-dugXIK_YKLf0WgfzdSPvBMp69gawgp2XF_Bl7DE19tw-YXagOiTW7b8Hw6FNDfwNnxXQejjNpgdWDtEjP_hjV9o6xnbc0aAivo6MVvRmASzZ1f_rytYsHwrCFLF-n65i1cnX69PJnEwzaFuFRWt7EPWjhpUfqlSLLgdChcpkvuTSIxSQpeBmXxys-kKaRPg5Noxjn3knPr0LHLHdio6sq_B5Z6Z9KyUFJ4qUKQtpjh3ffCuOAwI-IRfFrJM5_3oBl5V-xOVU6Sz0nyOUk-gp1e3COf1rYnHKyJ__45GG-m2kSwt1JHPty6RT6ekQgORypeF6qBuMrXy0WeJdagg1YRsP9wYALKJeEwRvCu1-6Db8ikyLSNIFvT-8hAWN3rlOr2psPsFpKQGM3uo699CJuTy-l5fn528X0PXvRT_9Q5sw8bbbP0Bxj_tMWH7pT_ASkEBRE |
linkToPdf | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV3db9QwDLdgSGMSQjAYlDGWBySeqmuSNh9PaGKcNmATD0y6tyrtJdpgtLde74H_HrvtdTsEPNutWtuxndj5GeCtctbbeXCxpZ1Oqh2PMYkt48J5w5V2ReB0d_jsXJ1cpJ9m2WyAFFoObZVrn9g56nld0hn5RKDfNFJZPQlDV8TX4-n7xU1MA6So0DpM07gPDzBIJjTMQM_0Lf5uYnr4TDqZsyJZ4zdm6aT4Xpd0ac8SjIHYiE8juuajhVuizEI_7OJv2eifTZV3otT0CTwe0kt21NvDU7jnq114eEwtQTTVbRe2z4Zi-jM4PbruIJVRM6wOrF39xKdZ0zfNetbWrCHggo5e_GIELNFc-9WPqyoWDO0OWbqs1zfP4WL68duHk3iYrBCXqVVt7IMSTlrURCkSHZwKhdOq5N4kEjdMwcuQWlz-c2kK6bPgJLp0zr3k3DoM8nIPtqq68i-BZd6ZrCxSKbxMQ5C2mKMf8MK44HB3xCN4t5ZnvugBNPKu8J2lOUk-J8nnJPkI9npxj3xK2Z5wsCH-2_dg7pkpE8H-Wh35sAKX-WgvERyOVFw6VA9xla9Xy1wn1mCwTiNg_-DAzSiXhMkYwYteu3f-QUuhlY1Ab-h9ZCDc7k1KdXXZ4XcLSaiM5tV_P_sQttHA8y-n55_3YacHAKAmmtew1TYrf4CpUFu86Yz8N4zsCXI |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Alteration+of+tumour+response+to+radiation+by+interleukin-2+gene+transfer&rft.jtitle=British+journal+of+cancer&rft.au=Lee%2C+J&rft.au=Moran%2C+J+P&rft.au=Fenton%2C+B+M&rft.au=Koch%2C+C+J&rft.date=2000-02-01&rft.issn=0007-0920&rft.volume=82&rft.issue=4&rft.spage=937&rft.epage=944&rft_id=info:doi/10.1054%2Fbjoc.1999.1022&rft.externalDBID=NO_FULL_TEXT |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0007-0920&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0007-0920&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0007-0920&client=summon |