Histological observations of dental tissues using the confocal laser scanning microscope

• Published in: Biotechnic & histochemistry Vol. 70; no. 2; p. 66
• Main Authors: Kabasawa, M, Ejiri, S, Hanada, K, Ozawa, H
• Format: Journal Article
• Language: English
• Published: England 1995
• Subjects: Animals; Dental Cementum - anatomy & histology; Dental Enamel - anatomy & histology; Dental Pulp Cavity - anatomy & histology; Dentin - anatomy & histology; Male; Maxilla - anatomy & histology; Microscopy, Confocal - methods; Molar - anatomy & histology; Rats; Rats, Inbred F344

To investigate the time course of mineralization in undecalcified dental tissues, calcein- and tetracycline-labeled rat maxillary molar sections were stained with Villanueva bone stain en bloc, embedded in methyl-methacrylate (MMA), ground to 50 microns thickness, and observed by confocal laser scan...