Oligonucleotide Functionalised Microbeads: Indispensable Tools for High-Throughput Aptamer Selection

• Published in: Molecules (Basel, Switzerland) Vol. 20; no. 12; pp. 21298 - 21312
• Main Authors: Fraser, Lewis, Kinghorn, Andrew, Tang, Marco, Cheung, Yee-Wai, Lim, Bryce, Liang, Shaolin, Dirkzwager, Roderick, Tanner, Julian
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01.12.2015; MDPI
• Subjects: aptamer; Aptamers, Nucleotide - chemistry; Candidates; ePCR; Evolution; Experiments; FACS; High-Throughput Screening Assays - methods; Humans; Libraries; microbead; Microspheres; monoclonal bead; Oligonucleotides - chemistry; Review; SELEX; SELEX Aptamer Technique - methods; microbead; FACS; aptamer; ePCR; monoclonal bead; SELEX

The functionalisation of microbeads with oligonucleotides has become an indispensable technique for high-throughput aptamer selection in SELEX protocols. In addition to simplifying the separation of binding and non-binding aptamer candidates, microbeads have facilitated the integration of other tech...