Use of multiplex PCR in diagnosis of childhood acute viral diarrhoea caused by rotavirus, norovirus, astrovirus and adenovirus in Upper Egypt

• Published in: Egyptian Journal of Medical Human Genetics Vol. 23; no. 1; pp. 40 - 9
• Main Authors: Othma, Amr Abulhamd Sayed, Gomaa, Howayda Ezz Eldin, El Anany, Mervat Gaber, Abdul Rahman, Eiman Mohammed, Hassan, Eman Mahmoud, Abd Elbaky, Abeer M. Nour Eldin, Soliman, May Mohamed Sherif, Awadallah, Eman
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 03.03.2022; Springer; Springer Nature B.V; SpringerOpen
• Subjects: Adenoviruses; Astrovirus; Childhood; Children; Developing countries; Diagnosis; Diarrhea; Egypt; Electron microscopy; Gastroenteritis; Health aspects; LDCs; Medicine; Medicine & Public Health; Mixed infection; Morbidity; Mortality; Multiplex PCR; Norovirus; Polymerase chain reaction; Primary care; Public health; Reverse transcription; Rotavirus; South Korea; Viruses; South Korea; Egypt; Multiplex PCR; Astrovirus; Norovirus; Rotavirus; Gastroenteritis

Background Diarrhoea is still a major public health issue in developing countries, and it is one of the leading causes of morbidity and mortality in children. We aimed to assess the use of a multiplex reverse transcription polymerase chain reaction (RT-PCR) assay for the detection of five viruses, i...