Understanding the role of dynamics in the iron sulfur cluster molecular machine

• Published in: Biochimica et biophysica acta Vol. 1861; no. 1; pp. 3154 - 3163
• Main Authors: di Maio, Danilo, Chandramouli, Balasubramanian, Yan, Robert, Brancato, Giuseppe, Pastore, Annalisa
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.01.2017; Elsevier Pub. Co
• Subjects: bacterial proteins; Biocatalysis; Carbon-Sulfur Lyases - chemistry; Carbon-Sulfur Lyases - metabolism; crystal structure; CyaY; Escherichia coli - metabolism; Escherichia coli Proteins - chemistry; Escherichia coli Proteins - metabolism; Frataxin; Iron-sulfur cluster biogenesis; Iron-Sulfur Proteins - chemistry; Iron-Sulfur Proteins - metabolism; Magnetic Resonance Spectroscopy; Molecular dynamics; nuclear magnetic resonance spectroscopy; prostheses; Protein Stability; Protein Structure, Secondary; Static Electricity; Structure; sulfur; NMR; Iron-sulfur cluster biogenesis; Frataxin; MD; RMSF; Molecular dynamics; RMSD; Structure; CyaY; PCA; ED

The bacterial proteins IscS, IscU and CyaY, the bacterial orthologue of frataxin, play an essential role in the biological machine that assembles the prosthetic FeS cluster groups on proteins. They form functionally binary and ternary complexes both in vivo and in vitro. Yet, the mechanism by which...