Biofilm Formation by Moraxella catarrhalis In Vitro: Roles of the UspA1 Adhesin and the Hag Hemagglutinin

• Published in: Infection and Immunity Vol. 74; no. 3; pp. 1588 - 1596
• Main Authors: Pearson, Melanie M, Laurence, Cassie A, Guinn, Sarah E, Hansen, Eric J
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 01.03.2006
• Subjects: Adhesins, Bacterial; Bacterial Adhesion - physiology; Bacterial Outer Membrane Proteins - genetics; Bacterial Outer Membrane Proteins - immunology; Bacterial Outer Membrane Proteins - physiology; Bacterial Proteins - genetics; Bacterial Proteins - immunology; Bacterial Proteins - physiology; Biofilms - growth & development; Cell Line; Conjunctivitis, Bacterial; Epithelial Cells; Hemagglutination Tests; Hemagglutinins - genetics; Hemagglutinins - immunology; Molecular Pathogenesis; Moraxella (Branhamella) catarrhalis - genetics; Moraxella (Branhamella) catarrhalis - immunology; Moraxella (Branhamella) catarrhalis - physiology; Moraxella catarrhalis

Mutant analysis was used to identify Moraxella catarrhalis gene products necessary for biofilm development in a crystal violet-based assay involving 24-well tissue culture plates. The wild-type M. catarrhalis strains that formed the most extensive biofilms in this system proved to be refractory to t...