Accurate detection of circulating tumor DNA using nanopore consensus sequencing
Levels of circulating tumor DNA (ctDNA) in liquid biopsies may serve as a sensitive biomarker for real-time, minimally-invasive tumor diagnostics and monitoring. However, detecting ctDNA is challenging, as much fewer than 5% of the cell-free DNA in the blood typically originates from the tumor. To d...
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Published in | Npj genomic medicine Vol. 6; no. 1; p. 106 |
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Main Authors | , , , , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
England
Nature Publishing Group
09.12.2021
Nature Publishing Group UK Nature Portfolio |
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Abstract | Levels of circulating tumor DNA (ctDNA) in liquid biopsies may serve as a sensitive biomarker for real-time, minimally-invasive tumor diagnostics and monitoring. However, detecting ctDNA is challenging, as much fewer than 5% of the cell-free DNA in the blood typically originates from the tumor. To detect lowly abundant ctDNA molecules based on somatic variants, extremely sensitive sequencing methods are required. Here, we describe a new technique, CyclomicsSeq, which is based on Oxford Nanopore sequencing of concatenated copies of a single DNA molecule. Consensus calling of the DNA copies increased the base-calling accuracy ~60×, enabling accurate detection of TP53 mutations at frequencies down to 0.02%. We demonstrate that a TP53-specific CyclomicsSeq assay can be successfully used to monitor tumor burden during treatment for head-and-neck cancer patients. CyclomicsSeq can be applied to any genomic locus and offers an accurate diagnostic liquid biopsy approach that can be implemented in clinical workflows. |
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AbstractList | Levels of circulating tumor DNA (ctDNA) in liquid biopsies may serve as a sensitive biomarker for real-time, minimally-invasive tumor diagnostics and monitoring. However, detecting ctDNA is challenging, as much fewer than 5% of the cell-free DNA in the blood typically originates from the tumor. To detect lowly abundant ctDNA molecules based on somatic variants, extremely sensitive sequencing methods are required. Here, we describe a new technique, CyclomicsSeq, which is based on Oxford Nanopore sequencing of concatenated copies of a single DNA molecule. Consensus calling of the DNA copies increased the base-calling accuracy ~60×, enabling accurate detection of TP53 mutations at frequencies down to 0.02%. We demonstrate that a TP53-specific CyclomicsSeq assay can be successfully used to monitor tumor burden during treatment for head-and-neck cancer patients. CyclomicsSeq can be applied to any genomic locus and offers an accurate diagnostic liquid biopsy approach that can be implemented in clinical workflows. Abstract Levels of circulating tumor DNA (ctDNA) in liquid biopsies may serve as a sensitive biomarker for real-time, minimally-invasive tumor diagnostics and monitoring. However, detecting ctDNA is challenging, as much fewer than 5% of the cell-free DNA in the blood typically originates from the tumor. To detect lowly abundant ctDNA molecules based on somatic variants, extremely sensitive sequencing methods are required. Here, we describe a new technique, CyclomicsSeq, which is based on Oxford Nanopore sequencing of concatenated copies of a single DNA molecule. Consensus calling of the DNA copies increased the base-calling accuracy ~60×, enabling accurate detection of TP53 mutations at frequencies down to 0.02%. We demonstrate that a TP53-specific CyclomicsSeq assay can be successfully used to monitor tumor burden during treatment for head-and-neck cancer patients. CyclomicsSeq can be applied to any genomic locus and offers an accurate diagnostic liquid biopsy approach that can be implemented in clinical workflows. Levels of circulating tumor DNA (ctDNA) in liquid biopsies may serve as a sensitive biomarker for real-time, minimally-invasive tumor diagnostics and monitoring. However, detecting ctDNA is challenging, as much fewer than 5% of the cell-free DNA in the blood typically originates from the tumor. To detect lowly abundant ctDNA molecules based on somatic variants, extremely sensitive sequencing methods are required. Here, we describe a new technique, CyclomicsSeq, which is based on Oxford Nanopore sequencing of concatenated copies of a single DNA molecule. Consensus calling of the DNA copies increased the base-calling accuracy ~60×, enabling accurate detection of TP53 mutations at frequencies down to 0.02%. We demonstrate that a TP53 -specific CyclomicsSeq assay can be successfully used to monitor tumor burden during treatment for head-and-neck cancer patients. CyclomicsSeq can be applied to any genomic locus and offers an accurate diagnostic liquid biopsy approach that can be implemented in clinical workflows. Abstract Levels of circulating tumor DNA (ctDNA) in liquid biopsies may serve as a sensitive biomarker for real-time, minimally-invasive tumor diagnostics and monitoring. However, detecting ctDNA is challenging, as much fewer than 5% of the cell-free DNA in the blood typically originates from the tumor. To detect lowly abundant ctDNA molecules based on somatic variants, extremely sensitive sequencing methods are required. Here, we describe a new technique, CyclomicsSeq, which is based on Oxford Nanopore sequencing of concatenated copies of a single DNA molecule. Consensus calling of the DNA copies increased the base-calling accuracy ~60×, enabling accurate detection of TP53 mutations at frequencies down to 0.02%. We demonstrate that a TP53 -specific CyclomicsSeq assay can be successfully used to monitor tumor burden during treatment for head-and-neck cancer patients. CyclomicsSeq can be applied to any genomic locus and offers an accurate diagnostic liquid biopsy approach that can be implemented in clinical workflows. |
ArticleNumber | 106 |
Author | Peltenburg, Boris Devriese, Lot A de Ridder, Jeroen de Bree, Remco van Ginkel, Joost H Chen, Li-Ting Willems, Stefan M Kloosterman, Wigard P Renkens, Ivo Straver, Roy Terhaard, Chris Marcozzi, Alessio Jager, Myrthe Elferink, Martin van Kuik, Joyce |
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Cites_doi | 10.18632/oncotarget.20004 10.1038/nature14129 10.1073/pnas.78.8.4833 10.1056/NEJMoa1213261 10.1038/nrc2982 10.1038/cdd.2017.180 10.1101/gr.113985.110 10.1038/s41598-018-29334-5 10.1021/acs.analchem.9b00856 10.1186/s13742-016-0140-7 10.1093/nar/gky1015 10.1016/j.molonc.2014.12.003 10.1038/s41586-019-1272-6 10.1093/bioinformatics/btv098 10.1186/s12885-017-3424-0 10.3390/cancers3010994 10.18632/oncotarget.11196 10.1038/nm.1789 10.1038/s41592-019-0507-7 10.1126/scitranslmed.3007094 10.1007/s10555-020-09876-9 10.1086/302205 10.1101/cshperspect.a001008 10.1038/nm.3519 10.1021/ac202028g 10.1038/nrc.2018.11 10.1016/S1470-2045(13)70546-1 10.1038/nrc.2017.7 10.1038/nrclinonc.2013.110 10.1186/s13059-015-0670-9 10.1038/srep11198 10.1073/pnas.1806447115 10.1038/nbt.3423 10.1126/scitranslmed.aat4921 10.1038/nbt.3520 10.1038/nature12861 10.1126/scitranslmed.3004742 10.1101/645903 |
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References | 37286583 - NPJ Genom Med. 2023 Jun 7;8(1):12 BJ Hindson (272_CR15) 2011; 83 AM Newman (272_CR16) 2016; 34 SM Kiełbasa (272_CR40) 2011; 21 MC Frith (272_CR41) 2015; 16 Cancer Genome Atlas Network. (272_CR33) 2015; 517 AD Tyler (272_CR20) 2018; 8 T Ozaki (272_CR28) 2011; 3 R Volden (272_CR23) 2018; 115 CR Leemans (272_CR31) 2011; 11 F Mouliere (272_CR19) 2018; 10 C Li (272_CR22) 2016; 5 E Crowley (272_CR6) 2013; 10 M Olivier (272_CR27) 2010; 2 JH van Ginkel (272_CR34) 2016; 7 D Deamer (272_CR17) 2016; 34 EH Baugh (272_CR29) 2018; 25 JG Tate (272_CR38) 2019; 47 YM Lo (272_CR1) 1999; 64 D Shore (272_CR26) 1981; 78 272_CR39 JH van Ginkel (272_CR14) 2017; 17 272_CR36 F Diehl (272_CR8) 2008; 14 272_CR37 A Tarasov (272_CR42) 2015; 31 272_CR12 JCM Wan (272_CR2) 2017; 17 272_CR10 A Acevedo (272_CR24) 2014; 505 R De Angelis (272_CR30) 2014; 15 S Perdomo (272_CR35) 2017; 8 S Wei (272_CR18) 2018; 8 S-J Dawson (272_CR4) 2013; 368 R Lebofsky (272_CR3) 2015; 9 SC-H Tsao (272_CR7) 2015; 5 S Cristiano (272_CR13) 2019; 570 M Eisenstein (272_CR21) 2019; 16 272_CR25 RJ Leary (272_CR11) 2012; 4 CR Leemans (272_CR32) 2018; 18 AM Newman (272_CR9) 2014; 20 C Bettegowda (272_CR5) 2014; 6 |
References_xml | – volume: 8 start-page: 72621 year: 2017 ident: 272_CR35 publication-title: Oncotarget doi: 10.18632/oncotarget.20004 contributor: fullname: S Perdomo – volume: 517 start-page: 576 year: 2015 ident: 272_CR33 publication-title: Nature doi: 10.1038/nature14129 contributor: fullname: Cancer Genome Atlas Network. – volume: 78 start-page: 4833 year: 1981 ident: 272_CR26 publication-title: Proc. Natl Acad. Sci. USA doi: 10.1073/pnas.78.8.4833 contributor: fullname: D Shore – volume: 368 start-page: 1199 year: 2013 ident: 272_CR4 publication-title: N. Engl. J. Med. doi: 10.1056/NEJMoa1213261 contributor: fullname: S-J Dawson – volume: 11 start-page: 9 year: 2011 ident: 272_CR31 publication-title: Nat. Rev. Cancer doi: 10.1038/nrc2982 contributor: fullname: CR Leemans – ident: 272_CR12 – ident: 272_CR37 – volume: 25 start-page: 154 year: 2018 ident: 272_CR29 publication-title: Cell Death Differ. doi: 10.1038/cdd.2017.180 contributor: fullname: EH Baugh – volume: 21 start-page: 487 year: 2011 ident: 272_CR40 publication-title: Genome Res. doi: 10.1101/gr.113985.110 contributor: fullname: SM Kiełbasa – volume: 8 start-page: 1 year: 2018 ident: 272_CR20 publication-title: Sci. Rep. doi: 10.1038/s41598-018-29334-5 contributor: fullname: AD Tyler – ident: 272_CR25 doi: 10.1021/acs.analchem.9b00856 – volume: 5 year: 2016 ident: 272_CR22 publication-title: Gigascience doi: 10.1186/s13742-016-0140-7 contributor: fullname: C Li – volume: 47 start-page: D941 year: 2019 ident: 272_CR38 publication-title: Nucleic Acids Res. doi: 10.1093/nar/gky1015 contributor: fullname: JG Tate – volume: 9 start-page: 783 year: 2015 ident: 272_CR3 publication-title: Mol. Oncol. doi: 10.1016/j.molonc.2014.12.003 contributor: fullname: R Lebofsky – volume: 570 start-page: 385 year: 2019 ident: 272_CR13 publication-title: Nature doi: 10.1038/s41586-019-1272-6 contributor: fullname: S Cristiano – volume: 31 start-page: 2032 year: 2015 ident: 272_CR42 publication-title: Bioinformatics doi: 10.1093/bioinformatics/btv098 contributor: fullname: A Tarasov – volume: 17 year: 2017 ident: 272_CR14 publication-title: BMC Cancer doi: 10.1186/s12885-017-3424-0 contributor: fullname: JH van Ginkel – volume: 3 start-page: 994 year: 2011 ident: 272_CR28 publication-title: Cancers doi: 10.3390/cancers3010994 contributor: fullname: T Ozaki – volume: 7 start-page: 61575 year: 2016 ident: 272_CR34 publication-title: Oncotarget doi: 10.18632/oncotarget.11196 contributor: fullname: JH van Ginkel – volume: 14 start-page: 985 year: 2008 ident: 272_CR8 publication-title: Nat. Med. doi: 10.1038/nm.1789 contributor: fullname: F Diehl – volume: 16 start-page: 683 year: 2019 ident: 272_CR21 publication-title: Nat. Methods doi: 10.1038/s41592-019-0507-7 contributor: fullname: M Eisenstein – volume: 6 start-page: 224ra24 year: 2014 ident: 272_CR5 publication-title: Sci. Transl. Med. doi: 10.1126/scitranslmed.3007094 contributor: fullname: C Bettegowda – ident: 272_CR10 doi: 10.1007/s10555-020-09876-9 – volume: 8 start-page: 1649 year: 2018 ident: 272_CR18 publication-title: Genetics contributor: fullname: S Wei – ident: 272_CR36 – volume: 64 start-page: 218 year: 1999 ident: 272_CR1 publication-title: Am. J. Hum. Genet. doi: 10.1086/302205 contributor: fullname: YM Lo – volume: 2 start-page: a001008 year: 2010 ident: 272_CR27 publication-title: Cold Spring Harb. Perspect. Biol. doi: 10.1101/cshperspect.a001008 contributor: fullname: M Olivier – volume: 20 start-page: 548 year: 2014 ident: 272_CR9 publication-title: Nat. Med. doi: 10.1038/nm.3519 contributor: fullname: AM Newman – volume: 83 start-page: 8604 year: 2011 ident: 272_CR15 publication-title: Anal. Chem. doi: 10.1021/ac202028g contributor: fullname: BJ Hindson – volume: 18 start-page: 269 year: 2018 ident: 272_CR32 publication-title: Nat. Rev. Cancer doi: 10.1038/nrc.2018.11 contributor: fullname: CR Leemans – volume: 15 start-page: 23 year: 2014 ident: 272_CR30 publication-title: Lancet Oncol. doi: 10.1016/S1470-2045(13)70546-1 contributor: fullname: R De Angelis – volume: 17 start-page: 223 year: 2017 ident: 272_CR2 publication-title: Nat. Rev. Cancer doi: 10.1038/nrc.2017.7 contributor: fullname: JCM Wan – volume: 10 start-page: 472 year: 2013 ident: 272_CR6 publication-title: Nat. Rev. Clin. Oncol. doi: 10.1038/nrclinonc.2013.110 contributor: fullname: E Crowley – volume: 16 year: 2015 ident: 272_CR41 publication-title: Genome Biol. doi: 10.1186/s13059-015-0670-9 contributor: fullname: MC Frith – volume: 5 year: 2015 ident: 272_CR7 publication-title: Sci. Rep. doi: 10.1038/srep11198 contributor: fullname: SC-H Tsao – volume: 115 start-page: 9726 year: 2018 ident: 272_CR23 publication-title: Proc. Natl Acad. Sci. USA doi: 10.1073/pnas.1806447115 contributor: fullname: R Volden – volume: 34 start-page: 518 year: 2016 ident: 272_CR17 publication-title: Nat. Biotechnol. doi: 10.1038/nbt.3423 contributor: fullname: D Deamer – volume: 10 start-page: eaat4921 year: 2018 ident: 272_CR19 publication-title: Sci. Transl. Med. doi: 10.1126/scitranslmed.aat4921 contributor: fullname: F Mouliere – volume: 34 start-page: 547 year: 2016 ident: 272_CR16 publication-title: Nat. Biotechnol. doi: 10.1038/nbt.3520 contributor: fullname: AM Newman – volume: 505 start-page: 686 year: 2014 ident: 272_CR24 publication-title: Nature doi: 10.1038/nature12861 contributor: fullname: A Acevedo – volume: 4 start-page: 162ra154 year: 2012 ident: 272_CR11 publication-title: Sci. Transl. Med. doi: 10.1126/scitranslmed.3004742 contributor: fullname: RJ Leary – ident: 272_CR39 doi: 10.1101/645903 |
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Snippet | Levels of circulating tumor DNA (ctDNA) in liquid biopsies may serve as a sensitive biomarker for real-time, minimally-invasive tumor diagnostics and... Abstract Levels of circulating tumor DNA (ctDNA) in liquid biopsies may serve as a sensitive biomarker for real-time, minimally-invasive tumor diagnostics and... Abstract Levels of circulating tumor DNA (ctDNA) in liquid biopsies may serve as a sensitive biomarker for real-time, minimally-invasive tumor diagnostics and... |
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SubjectTerms | Biomarkers Biopsy Cancer therapies Data processing Deoxyribonucleic acid DNA DNA sequencing Head & neck cancer Human papillomavirus Medical prognosis Mutation Patients Tumors |
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Title | Accurate detection of circulating tumor DNA using nanopore consensus sequencing |
URI | https://www.ncbi.nlm.nih.gov/pubmed/34887408 https://www.proquest.com/docview/2608260703/abstract/ https://search.proquest.com/docview/2608537152 https://pubmed.ncbi.nlm.nih.gov/PMC8660781 https://doaj.org/article/876ba2e504214507ada4be457cb69876 |
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