Cell culture isolation and sequence analysis of genetically diverse US porcine epidemic diarrhea virus strains including a novel strain with a large deletion in the spike gene

• Published in: Veterinary microbiology Vol. 173; no. 3-4; pp. 258 - 269
• Main Authors: Oka, Tomoichiro, Saif, Linda J., Marthaler, Douglas, Esseili, Malak A., Meulia, Tea, Lin, Chun-Ming, Vlasova, Anastasia N., Jung, Kwonil, Zhang, Yan, Wang, Qiuhong
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 10.10.2014; Elsevier B.V. Published by Elsevier B.V
• Subjects: Animals; antiviral agents; Base Sequence; Canada; cell culture; Cell culture isolation; Cell Culture Techniques - veterinary; Chlorocebus aethiops; Complete genome analysis; Coronavirus Infections - epidemiology; Coronavirus Infections - veterinary; DNA primers; DNA Primers - genetics; financial economics; Fluorescent Antibody Technique, Indirect - veterinary; genes; Genetic Variation; immunoassays; Large deletion in S protein; live vaccines; Mexico; Microscopy, Electron, Transmission - veterinary; Midwestern United States - epidemiology; Molecular Sequence Data; mortality; nucleotide sequences; PEDV variants; Phylogeny; piglets; Porcine epidemic diarrhea virus; Porcine epidemic diarrhea virus (PEDV); Porcine epidemic diarrhea virus - genetics; Porcine epidemic diarrhea virus - isolation & purification; Porcine epidemic diarrhea virus - pathogenicity; Real-Time Polymerase Chain Reaction - veterinary; Reverse Transcriptase Polymerase Chain Reaction - veterinary; sequence analysis; Sequence Analysis, DNA - veterinary; Sequence Deletion - genetics; Species Specificity; Spike Glycoprotein, Coronavirus - genetics; Swine; Swine Diseases - epidemiology; Swine Diseases - virology; trypsin; United States; Vero Cells; Virulence; viruses; Midwestern United States; Canada; United States; Mexico; Porcine epidemic diarrhea virus (PEDV); Cell culture isolation; Complete genome analysis; Large deletion in S protein; PEDV variants

•We isolated a novel PEDV strain with a large deletion (197aa) in the spike gene.•We isolated US PEDV INDEL variant in Vero cells.•We isolated multiple original highly virulent US PEDV strains in Vero cells.•Genomic sequences of the isolated PEDV strains were analyzed.•The isolated genetically diver...