Cell culture isolation and sequence analysis of genetically diverse US porcine epidemic diarrhea virus strains including a novel strain with a large deletion in the spike gene - Oka, Tomoichiro; Saif, Linda J.; Marthaler, Douglas; Esseili, Malak A.; Meulia, Tea; Lin, Chun-Ming; Vlasova, Anastasia N.; Jung, Kwonil; Zhang, Yan; Wang, Qiuhong (Veterinary microbiology, 2014)
Keywords: Animals; antiviral agents; Base Sequence; Canada; cell culture; Cell culture isolation; Cell Culture Techniques - veterinary; Chlorocebus aethiops; Complete genome analysis; Coronavirus Infections - epidemiology; Coronavirus Infections - veterinary; DNA primers; DNA Primers - genetics; financial economics; Fluorescent Antibody Technique, Indirect - veterinary; genes; Genetic Variation; immunoassays; Large deletion in S protein; live vaccines; Mexico; Microscopy, Electron, Transmission - veterinary; Midwestern United States - epidemiology; Molecular Sequence Data; mortality; nucleotide sequences; PEDV variants; Phylogeny; piglets; Porcine epidemic diarrhea virus; Porcine epidemic diarrhea virus (PEDV); Porcine epidemic diarrhea virus - genetics; Porcine epidemic diarrhea virus - isolation & purification; Porcine epidemic diarrhea virus - pathogenicity; Real-Time Polymerase Chain Reaction - veterinary; Reverse Transcriptase Polymerase Chain Reaction - veterinary; sequence analysis; Sequence Analysis, DNA - veterinary; Sequence Deletion - genetics; Species Specificity; Spike Glycoprotein, Coronavirus - genetics; Swine; Swine Diseases - epidemiology; Swine Diseases - virology; trypsin; United States; Vero Cells; Virulence; viruses; Midwestern United States; Canada; United States; Mexico; Porcine epidemic diarrhea virus (PEDV); Cell culture isolation; Complete genome analysis; Large deletion in S protein; PEDV variants