The 3′-end region of the human PDGFR-β core promoter nuclease hypersensitive element forms a mixture of two unique end-insertion G-quadruplexes

• Published in: Biochimica et biophysica acta. General subjects Vol. 1862; no. 4; pp. 846 - 854
• Main Authors: Onel, Buket, Carver, Megan, Agrawal, Prashansa, Hurley, Laurence H., Yang, Danzhou
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.04.2018
• Subjects: Base Sequence; Circular Dichroism; circular dichroism spectroscopy; DNA - chemistry; DNA - genetics; Drug target; electrophoresis; End-insertion G-quadruplexes; G-quadruplex; G-Quadruplexes; guanine; Guanine - chemistry; Humans; nuclear magnetic resonance spectroscopy; Nucleic Acid Denaturation; PDGFR-β gene downregulation; platelet-derived growth factor receptor beta; promoter regions; Promoter Regions, Genetic - genetics; Receptor, Platelet-Derived Growth Factor beta - genetics; repressor proteins; Sequence Homology, Nucleic Acid; thermal stability; Transition Temperature; Drug target; PDGFR-β gene downregulation; End-insertion G-quadruplexes; G-quadruplex

While the most stable G-quadruplex formed in the human PDGFR-β promoter nuclease hypersensitive element (NHE) is the 5′-mid G-quadruplex, the 3′-end sequence that contains a 3′-GGA run forms a less stable G-quadruplex. Recently, the 3′-end G-quadruplex was found to be a transcriptional repressor and...