Regulation of rDNA transcription by insulin in primary cultures of rat hepatocytes

• Published in: The Journal of biological chemistry Vol. 268; no. 34; pp. 25277 - 25284
• Main Authors: Antonetti, D A, Kimball, S R, Horetsky, R L, Jefferson, L S
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Elsevier Inc 05.12.1993; American Society for Biochemistry and Molecular Biology
• Subjects: Animals; Biological and medical sciences; Cells, Cultured; DNA - metabolism; DNA, Ribosomal - metabolism; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation - drug effects; Insulin - pharmacology; Kinetics; Leucine - metabolism; Liver - drug effects; Liver - metabolism; Molecular and cellular biology; Molecular genetics; Protein Biosynthesis; Rats; Ribosomes - drug effects; Ribosomes - metabolism; Ribosomes - ultrastructure; RNA, Ribosomal - biosynthesis; Transcription, Genetic - drug effects; Transcription. Transcription factor. Splicing. Rna processing; Tritium; Uridine - metabolism; Uridine Triphosphate - metabolism; Protein hormone; Vertebrata; Mammalia; Ribosome; Transcription; Hepatocyte; Rat; Rodentia; Ribosomal DNA; Hormonal regulation; Insulin

The studies presented herein were designed to investigate the role of insulin in maintaining the steady-state number of ribosomes in primary cultures of rat hepatocytes. The RNA content of hepatocytes maintained in the presence of insulin did not change during the 7 days of culture. In contrast, the...