Calcium-Sensing Receptors Control CYP27B1-Luciferase Expression: Transcriptional and Posttranscriptional Mechanisms

• Published in: Journal of the Endocrine Society Vol. 5; no. 9; p. bvab057
• Main Authors: Huang, Alice, Binmahfouz, Lenah, Hancock, Dale P, Anderson, Paul H, Ward, Donald T, Conigrave, Arthur D
• Format: Journal Article
• Language: English
• Published: US Oxford University Press 01.09.2021
• Subjects: Cinacalcet; Ethylenediaminetetraacetic acid; Genetic aspects; Genetic transcription; Kinases; Luciferase; Protein biosynthesis; Protein kinases; Proteins; RNA; Australia; CYP27B1; ERK1/2; transcription; protein breakdown; Thr-888 phosphorylation; calcium-sensing receptor; PKC; protein synthesis; 1αOHase

25-hydroxyvitamin D 1α-hydroxylase (encoded by CYP27B1), which catalyzes the synthesis of 1,25-dihydroxyvitamin D3, is subject to negative or positive modulation by extracellular Ca2+ (Ca2+o) depending on the tissue. However, the Ca2+ sensors and underlying mechanisms are unidentified. We tested whe...