A mutation associated with centronuclear myopathy enhances the size and stability of dynamin 2 complexes in cells - James, Nicholas G.; Digman, Michelle A.; Ross, Justin A.; Barylko, Barbara; Wang, Lei; Li, Jinhui; Chen, Yan; Mueller, Joachim D.; Gratton, Enrico; Albanesi, Joseph P.; Jameson, David M. (Biochimica et biophysica acta, 2014)
Keywords: Animals; Bone Neoplasms - genetics; Bone Neoplasms - metabolism; Bone Neoplasms - pathology; Cell Membrane - metabolism; Cells, Cultured; Centronuclear myopathy; Clathrin - metabolism; cytosol; Cytosol - metabolism; Dynamin 2; Dynamin II - genetics; Dynamin II - metabolism; dynamins; EGFP; Embryo, Mammalian - cytology; Embryo, Mammalian - metabolism; Endocytosis; Fibroblasts - cytology; Fibroblasts - metabolism; fluorescence; Fluorescence fluctuation spectroscopy; Golgi Apparatus - metabolism; Humans; Image Processing, Computer-Assisted; Mice; Microscopy, Fluorescence; muscular diseases; mutants; mutation; Mutation - genetics; Myopathies, Structural, Congenital - genetics; Myopathies, Structural, Congenital - metabolism; Myopathies, Structural, Congenital - pathology; Osteosarcoma - genetics; Osteosarcoma - metabolism; Osteosarcoma - pathology; patients; physiological transport; plasma membrane; polymers; Protein Multimerization - genetics; Protein Transport; R369W mutation; spectroscopy; TIRF; PSF; CNM; ACF; Fluorescence fluctuation spectroscopy; GED; R369W; N&B; EMCCD; EGFP; TIRF; MEF; FFS; R369W mutation; PCH; Dyn2-EGFP; Dyn; PH; ICS; CME; Centronuclear myopathy; Dynamin 2; wt; PM; Mouse embryo fibroblasts; rat dynamin 2 isoform 2ba with a C- terminal EGFP and terminal hexa-histidine tag; point spread function; Number and Brightness; autocorrelation function; Pleckstrin Homology; Image Correlation Spectroscopy; Clathrin-mediated endocytosis; plasma membrane; GTPase effector domain; Photon Counting Histogram; Total Internal Reflection Fluorescence; rat dynamin 2 isoform 2ba construct containing an Arg to Trp mutation at residue 369; electron multiplying charge-coupled device; Dynamin; wild-type