Evaluation of Three Influenza A and B Real-Time Reverse Transcription-PCR Assays and a New 2009 H1N1 Assay for Detection of Influenza Viruses

• Published in: Journal of Clinical Microbiology Vol. 48; no. 11; pp. 3870 - 3875
• Main Authors: Selvaraju, Suresh B, Selvarangan, Rangaraj
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.11.2010; American Society for Microbiology (ASM)
• Subjects: Biological and medical sciences; Fundamental and applied biological sciences. Psychology; Humans; Influenza A virus - isolation & purification; Influenza B virus - isolation & purification; Influenza virus; Influenza, Human - diagnosis; Influenza, Human - virology; Microbiology; Miscellaneous; Reagent Kits, Diagnostic; Reverse Transcriptase Polymerase Chain Reaction - methods; Sensitivity and Specificity; Virology; Virology - methods; Infection; Virus; Influenzavirus A; Influenza A virus; Viral disease; Orthomyxoviridae; Influenza A; Detection; Real time; Reverse transcription polymerase chain reaction; Influenza B

The performance characteristics of three real-time influenza A/B virus reverse transcription-PCR (RT-PCR) assays and two real-time 2009 H1N1 RT-PCR assays were evaluated using previously characterized clinical specimens. A total of 150 respiratory specimens from children (30 influenza A/H1 virus-, 3...