Comparative analysis of four complete mitogenomes from hoverfly genus Eristalinus with phylogenetic implications

• Published in: Scientific reports Vol. 12; no. 1; p. 4164
• Main Authors: Li, Hu, Li, Juan
• Format: Journal Article
• Language: English
• Published: England Nature Publishing Group 09.03.2022; Nature Publishing Group UK; Nature Portfolio
• Subjects: Bayesian analysis; Comparative analysis; Cyclooxygenase-2; Eristalinus; Genomes; Mitochondria; Phylogenetics; Phylogeny; rRNA 12S; rRNA 16S; Stop codon; Syrphidae; Transfer RNA; tRNA
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-022-08172-6

The genus Eristalinus is widely distributed globally. Four complete mitochondrial genomes (i.e., mitogenomes) of Eristalinus were sequenced and analyzed in this study: Eristalinus viridis (Coquillett, 1898), E. quinquestriatus (Fabricius, 1781), E. tarsalis (Macquart, 1855), and E. sp. Within these four sequenced mitogenomes, most protein-coding genes (ND2, CO1, COX2, COX3, ND3, ND5, ND4, ND4L, ND6, and Cytb) began with a typical ATN (T/C/G/A) start codon and ended with a stop codon TAA or incomplete T, whereas ND1 began with the start codon TTG. ND3 ended with TAG. The secondary tRNA structure was that of a typical cloverleaf, and only the tRNA-Ser1 lacked a DHU arm. Three and five domains appeared in the 12S and 16S rRNA secondary structures, respectively. The phylogenetic relationships among the four Eristalinus species combined with the published mitogenomes of Syrphidae were reconstructed using the maximum likelihood and Bayesian inference methods, which support the monophyly of the subfamily Syrphinae but do not support that of the subfamily Eristalinae. Of note, Eristalini and Syrphini are monophyletic groups. The mitogenomes of E. viridis, E. quinquestriatus, E. sp., and E. tarsalis are useful for determining the phylogenetic relationships and evolution of Syrphidae.