MicroRNA-148a-3p suppresses cell proliferation and migration of esophageal carcinoma by targeting CEP55

• Published in: Cellular & molecular biology letters Vol. 26; no. 1; p. 54
• Main Authors: Lin, Yong, Chen, Yushan, Shen, Rongqiang, Chen, Dingzhu, Lin, Yimin
• Format: Journal Article
• Language: English
• Published: England BioMed Central 24.12.2021; BMC
• Subjects: 1-Phosphatidylinositol 3-kinase; AKT protein; Animals; Apoptosis; Apoptosis - genetics; Binding sites; Bioinformatics; Cell cycle; Cell Cycle Proteins - genetics; Cell Cycle Proteins - metabolism; Cell Line, Tumor; Cell migration; Cell Movement - genetics; Cell proliferation; Cell Proliferation - genetics; CEP55; Cytology; Esophageal cancer; Esophageal carcinoma; Esophageal Neoplasms - genetics; Esophageal Neoplasms - pathology; Esophagus; Flow cytometry; Gene Expression Regulation, Neoplastic; Genes; Humans; Invasion; Liver cancer; Mice; Mice, Nude; microRNA-148a-3p; MicroRNAs; MicroRNAs - genetics; Migration; miRNA; Plasmids; Proliferation; Proteins; Research Letter; Signal transduction; Tumorigenesis; Xenograft Model Antitumor Assays; Beijing China; United States > US; China; Japan; Esophageal carcinoma; Migration; microRNA-148a-3p; Proliferation; CEP55; Invasion; Apoptosis
• ISSN: 1425-8153; 1689-1392
• DOI: 10.1186/s11658-021-00298-1

This study evaluated microRNA-148a-3p in esophageal carcinoma cells. The prediction of bioinformatics analysis revealed that microRNA-148a-3p may target CEP55. qRT-PCR and western blot showed that CEP55 level in esophageal carcinoma cells and tissue was dramatically higher than that of normal cells and tissue, while microRNA-148a-3p was the opposite. Forced expression of microRNA-148a-3p restrained cell malignant behaviors of esophageal carcinoma, and repression of microRNA-148a-3p resulted in the converse results in terms of cell function. Dual-luciferase assay confirmed that microRNA-148a-3p targeted CEP55. CEP55 attenuated the suppressive effect of microRNA-148a-3p on proliferation and migration of esophageal carcinoma cells, demonstrating that microRNA-148a-3p regulated function of esophageal carcinoma cells via decreasing CEP55 level. Microscopy observation indicated that cell morphology was also affected by the microRNA-148a-3p/CEP55 axis. Furthermore, western blot analysis revealed that the PI3K/AKT signaling pathway could be suppressed by activating the microRNA-148a-3p/CEP55 axis. Finally, in vivo experiments confirmed the effects of microRNA-148a-3p on tumorigenesis. Thus, microRNA-148a-3p could act as a repressor in esophageal carcinoma via binding to CEP55.