Optimization of the ultrafiltration-assisted extraction of Chinese yam polysaccharide using response surface methodology and its biological activity

• Published in: International Journal of Biological Macromolecules Vol. 121; pp. 1186 - 1193
• Main Authors: Xue, Heng-Yue, Li, Jing-Ru, Liu, Yan-Geng, Gao, Qi, Wang, Xiao-Wen, Zhang, Jun-Wei, Tanokura, Masaru, Xue, You-Lin
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.01.2019; Elsevier BV
• Subjects: 2,2-diphenyl-1-picrylhydrazyl; absorption; anion exchange; Antineoplastic Agents; Antineoplastic Agents - chemistry; Antineoplastic Agents - isolation & purification; Antineoplastic Agents - pharmacology; antioxidant activity; cell growth; Cell Line, Tumor; Cell Proliferation; Cell Proliferation - drug effects; Chinese yam polysaccharide; Dioscorea; Dioscorea - chemistry; Dioscorea oppositifolia; Dioscorea polystachya; experimental design; Fourier transform infrared spectroscopy; Free Radicals; Free Radicals - chemistry; galactose; glucose; Humans; Hydrogen-Ion Concentration; hydroxyl radicals; mannose; microstructure; Monosaccharides; Monosaccharides - analysis; Polysaccharides; Polysaccharides - chemistry; Polysaccharides - isolation & purification; Polysaccharides - pharmacology; powders; Pressure; raw materials; regression analysis; response surface methodology; Structural characterization and activities; temperature; Ultrafiltration; Ultrafiltration - methods; Ultrafiltration-assisted extraction; yams; Chinese yam polysaccharide; Structural characterization and activities; Ultrafiltration-assisted extraction

Ultrafiltration is a separation process for purifying and concentrating macromolecular solutions. Using Baiyu yam (Dioscorea opposita Thunb) as the raw material, single-factor experiments, Box–Behnken design (BBD) and response surface methodology (RSM) were employed to investigate the effects of the...