Droplet microfluidics-based high-throughput bacterial cultivation for validation of taxon pairs in microbial co-occurrence networks

• Published in: Scientific reports Vol. 12; no. 1; pp. 18145 - 13
• Main Authors: Jiang, Min-Zhi, Zhu, Hai-Zhen, Zhou, Nan, Liu, Chang, Jiang, Cheng-Ying, Wang, Yulin, Liu, Shuang-Jiang
• Format: Journal Article
• Language: English
• Published: England Nature Publishing Group 28.10.2022; Nature Publishing Group UK; Nature Portfolio
• Subjects: Bacteria; Bacteria - genetics; Cultivation; Ecosystems; Flowers & plants; Glycerol; Laboratories; Microbial activity; Microbial Consortia - genetics; Microbial Interactions; Microbiota - genetics; Microfluidics; Microfluidics - methods; Mutualism; Sediments; Taxa; Wetlands

Co-occurrence networks inferred from the abundance data of microbial communities are widely applied to predict microbial interactions. However, the high workloads of bacterial isolation and the complexity of the networks themselves constrained experimental demonstrations of the predicted microbial a...