Effects of cellulolytic ruminal bacteria and of cell extracts on germination of Euonymus americanus L. seeds

Astract: In past attempts, the experimental germination of the seeds of Euonymus americanus L. in vitro has had little success. However, treatment of seeds with ruminal fluid containing viable microflora has been successful in stimulating germination. In the presence of the cellulolytic ruminal bact...

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Published inApplied and Environmental Microbiology Vol. 54; no. 1; pp. 218 - 224
Main Authors Howard, G.T, Elliott, L.P
Format Journal Article
LanguageEnglish
Published Washington, DC American Society for Microbiology 01.01.1988
Subjects
Animal, plant and microbial ecology
BACTERIA
BACTERIE
Biological and medical sciences
CELASTRACEAE
Euonymus americanus
Fundamental and applied biological sciences. Psychology
GERMINACION
GERMINATION
Microbial ecology
Microorganism-Plant Interactions
MICROORGANISME
MICROORGANISMOS
Normal microflora of man and animals. Rumen
RUMEN
Eubacteriales
Enzymatic treatment
Germination
Celastraceae
Clostridiales
Microflora
Rumen
Peptococcaceae
Cellulolytic enzyme
Cellulase
Dicotyledones
Seed
Angiospermae
Clostridiaceae
Exo-cellobiohydrolase
Bacteria
Spermatophyta
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Summary:Astract: In past attempts, the experimental germination of the seeds of Euonymus americanus L. in vitro has had little success. However, treatment of seeds with ruminal fluid containing viable microflora has been successful in stimulating germination. In the presence of the cellulolytic ruminal bacterium, Clostridium cellobioparum ATCC 15832, seeds of E. americanus were stimulated to germinate. Subsequent studies were designed to determine whether the bacterium synthesized a cellulolyte enzyme responsible for initiating germination. The cell-free endocellulase from C. cellobioparum induced germination of the seeds. To support the hypothesis that the endocellulase from C. cellobioparum was responsible for triggering germination, a1,4-beta-d-glucan glucanohydrolase (EC 3.2.1.4) from Penicillum funiculosum was used to treat the seeds. In addition, no germination was obtained from seeds treated with a commerical exocellulase enzyme. Also, Ruminococcus flavefaciens FD-1 was found to initiate germination of E. americanus seeds. Thus, cellulase activity is indicated in the degradation of the testa of the seed, allowing imbibition and germination
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Present address: Department of Animal Sciences, University of Illinois, Urbana, IL 61801.
Corresponding author.
ISSN:0099-2240
1098-5336
DOI:10.1128/AEM.54.1.218-224.1988