Coexistence patterns of soil methanogens are closely tied to methane generation and community assembly in rice paddies

• Published in: Microbiome Vol. 9; no. 1; pp. 20 - 13
• Main Authors: Li, Dong, Ni, Haowei, Jiao, Shuo, Lu, Yahai, Zhou, Jizhong, Sun, Bo, Liang, Yuting
• Format: Journal Article
• Language: English
• Published: England BioMed Central 22.01.2021; BMC
• Subjects: Abiotic factors; Asia; Biogeography; Carbon; CH4 emission; Co-occurrence network; Coexistence; Common and endemic coexistence; Consortia; Deoxyribonucleic acid; DNA; Euryarchaeota - metabolism; Laboratories; Metabolism; Methane; Methane - biosynthesis; Methanobacterium - metabolism; Methanogenic bacteria; Methanogens; Oryza - microbiology; Potassium; Sediments; Soil Microbiology; Species diversity; Stochastic and deterministic processes; Stochastic models; Stochasticity; Asia; United States > US; China; Stochastic and deterministic processes; CH4 emission; Co-occurrence network; Methanogens; Common and endemic coexistence
• DOI: 10.1186/s40168-020-00978-8
• ISSN: 2049-2618

Soil methanogens participate in complex interactions, which determine the community structures and functions. Studies continue to seek the coexistence patterns of soil methanogens, influencing factors and the contribution to methane (CH ) production, which are regulated primarily by species interactions, and the functional significance of these interactions. Here, methane emissions were measured in rice paddies across the Asian continent, and the complex interactions involved in coexistence patterns of methanogenic archaeal communities were represented as pairwise links in co-occurrence networks. The network topological properties, which were positively correlated with mean annual temperature, were the most important predictor of CH emissions among all the biotic and abiotic factors. The methanogenic groups involved in commonly co-occurring links among the 39 local networks contributed most to CH emission (53.3%), much higher than the contribution of methanogenic groups with endemic links (36.8%). The potential keystone taxa, belonging to Methanobacterium, Methanocella, Methanothrix, and Methanosarcina, possessed high linkages with the methane generation functional genes mcrA, fwdB, mtbA, and mtbC. Moreover, the commonly coexisting taxa showed a very different assembly pattern, with ~ 30% determinism and ~ 70% stochasticity. In contrast, a higher proportion of stochasticity (93~99%) characterized the assembly of endemically coexisting taxa. These results suggest that the coexistence patterns of microbes are closely tied to their functional significance, and the potential importance of common coexistence further imply that complex networks of interactions may contribute more than species diversity to soil functions. Video abstract.