hexA of Erwinia carotovora ssp. carotovora strain Ecc71 negatively regulates production of RpoS and rsmB RNA, a global regulator of extracellular proteins, plant virulence and the quorum-sensing signal, N-(3-oxohexanoyl)-l-homoserine lactone
The soft‐rotting bacterium, Erwinia carotovora ssp. carotovora (E. c. carotovora ), produces an array of extracellular enzymes (= exoenzymes), including pectate lyase (Pel), polygalacturonase (Peh), cellulase (Cel) and protease (Prt), as well as HarpinEcc, the elicitor of hypersensitive reaction (HR...
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| Published in | Environmental microbiology Vol. 2; no. 2; pp. 203 - 215 |
|---|---|
| Main Authors | , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Oxford BSL
Blackwell Science Ltd
01.04.2000
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| Subjects | |
| Online Access | Get full text |
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| Abstract | The soft‐rotting bacterium, Erwinia carotovora ssp. carotovora (E. c. carotovora ), produces an array of extracellular enzymes (= exoenzymes), including pectate lyase (Pel), polygalacturonase (Peh), cellulase (Cel) and protease (Prt), as well as HarpinEcc, the elicitor of hypersensitive reaction (HR). The production of these exoenzymes and HarpinEcc responds to plant products and the quorum‐sensing signal [N‐(3‐oxohexanoyl)‐
l‐homoserine lactone; OHL] and is subject to both transcriptional and post‐transcriptional regulation. hexA of E. c. carotovora strain Ecc71 (hereafter hexA71), like that of another E. c. carotovora strain, negatively controls the production of exoenzymes, OHL and virulence in E. c. carotovora strain Ecc71. In addition to exoenzymes, HexA71 negatively regulates the expression of hrpNEcc, the structural gene for HarpinEcc. Exoenzyme overproduction is abolished by OHL deficiency in a HexA− and OhlI − double mutant, indicating that HexA and OHL are components of a common regulatory pathway controlling exoenzyme production. HexA71 negatively affects RpoS, as the levels of this alternative sigma factor are higher in the HexA− mutant than in the HexA+ strain. However, a HexA− and RpoS− double mutant produces higher levels of exoenzymes and transcripts of pel‐1, peh‐1 and celV genes than the HexA− and RpoS+ parent. Thus, the elevated levels of RpoS protein in the HexA− mutant do not account for exoenzyme overproduction. The following evidence associates for the first time the phenotypic changes in the HexA− mutant to overproduction of rsmB RNA, a global regulator of exoenzymes, HarpinEcc, OHL and secondary metabolites. Analyses of rsmB transcripts and expression of an rsmB–lacZ operon fusion in E. c. carotovora strain Ecc71 revealed that HexA71 negatively regulates transcription of rsmB. Multiple copies of hexA71+ DNA suppress various phenotypes, including exoenzyme production in E. c. carotovora strain Ecc71, and concomitantly inhibit the production of rsmB, pel‐1, peh‐1, celV and hrpNEcc transcripts. Multiple copies of rsmB+ DNA, on the other hand, stimulate exoenzyme production by relieving the negative effects of a chromosomal copy of hexA+. The occurrence of hexA homologues and the negative effect of the dosage of hexA71 DNA on rsmB transcripts were also detected in other E. c. carotovora strains as well as Erwinia carotovora atroseptica and Erwinia carotovora betavasculorum. Extrapolating from the findings with LrhA, the Escherichia coli homologue of HexA, and the presence of sprE homologues in E. carotovora subspecies, we propose that HexA71 controls several regulatory pathways in E. carotovora including rsmB transcription and the production of SprEEcc which, in turn, affects RpoS levels. A model is presented that integrates the findings presented here and our current knowledge of the major regulatory network that controls exoprotein production in soft‐rotting Erwinia carotovora subspecies. |
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| AbstractList | The soft-rotting bacterium, Erwinia carotovora ssp. carotovora (E. c. carotovora), produces an array of extracellular enzymes (= exoenzymes), including pectate lyase (Pel), polygalacturonase (Peh), cellulase (Cel) and protease (Prt), as well as HarpinEcc, the elicitor of hypersensitive reaction (HR). The production of these exoenzymes and HarpinEcc responds to plant products and the quorum-sensing signal [N-(3-oxohexanoyl)-L-homoserine lactone; OHL] and is subject to both transcriptional and post-transcriptional regulation. hexA of E. c. carotovora strain Ecc71 (hereafter hexA71), like that of another E. c. carotovora strain, negatively controls the production of exoenzymes, OHL and virulence in E. c. carotovora strain Ecc71. In addition to exoenzymes, HexA71 negatively regulates the expression of hrpNEcc, the structural gene for HarpinEcc. Exoenzyme overproduction is abolished by OHL deficiency in a HexA- and Ohll- double mutant, indicating that HexA and OHL are components of a common regulatory pathway controlling exoenzyme production. HexA71 negatively affects RpoS, as the levels of this alternative sigma factor are higher in the HexA- mutant than in the HexA+ strain. However, a HexA- and RpoS double mutant produces higher levels of exoenzymes and transcripts of pel-1, peh-1 and celVgenes than the HexA- and RpoS+ parent. Thus, the elevated levels of RpoS protein in the HexA- mutant do not account for exoenzyme overproduction. The following evidence associates for the first time the phenotypic changes in the HexA mutant to overproduction of rsmB RNA, a global regulator of exoenzymes, HarpinEcc, OHL and secondary metabolites. Analyses of rsmB transcripts and expression of an rsmB-lacZoperon fusion in E. c. carotovora strain Ecc71 revealed that HexA71 negatively regulates transcription of rsmB. Multiple copies of hexA71+ DNA suppress various phenotypes, including exoenzyme production in E. c. carotovora strain Ecc71, and concomitantly inhibit the production of rsmB, pel-1, peh-1, celV and hrpNEcc transcripts. Multiple copies of rsmB+ DNA, on the other hand, stimulate exoenzyme production by relieving the negative effects of a chromosomal copy of hexA+. The occurrence of hexA homologues and the negative effect of the dosage of hexA71 DNA on rsmB transcripts were also detected in other E. c. carotovora strains as well as Erwinia carotovora atroseptica and Erwinia carotovora betavasculorum. Extrapolating from the findings with LrhA, the Escherichia coli homologue of HexA, and the presence of sprE homologues in E. carotovora subspecies, we propose that HexA71 controls several regulatory pathways in E. carotovora including rsmB transcription and the production of SprEEcc which, in turn, affects RpoS levels. A model is presented that integrates the findings presented here and our current knowledge of the major regulatory network that controls exoprotein production in soft-rotting Erwinia carotovora subspecies.The soft-rotting bacterium, Erwinia carotovora ssp. carotovora (E. c. carotovora), produces an array of extracellular enzymes (= exoenzymes), including pectate lyase (Pel), polygalacturonase (Peh), cellulase (Cel) and protease (Prt), as well as HarpinEcc, the elicitor of hypersensitive reaction (HR). The production of these exoenzymes and HarpinEcc responds to plant products and the quorum-sensing signal [N-(3-oxohexanoyl)-L-homoserine lactone; OHL] and is subject to both transcriptional and post-transcriptional regulation. hexA of E. c. carotovora strain Ecc71 (hereafter hexA71), like that of another E. c. carotovora strain, negatively controls the production of exoenzymes, OHL and virulence in E. c. carotovora strain Ecc71. In addition to exoenzymes, HexA71 negatively regulates the expression of hrpNEcc, the structural gene for HarpinEcc. Exoenzyme overproduction is abolished by OHL deficiency in a HexA- and Ohll- double mutant, indicating that HexA and OHL are components of a common regulatory pathway controlling exoenzyme production. HexA71 negatively affects RpoS, as the levels of this alternative sigma factor are higher in the HexA- mutant than in the HexA+ strain. However, a HexA- and RpoS double mutant produces higher levels of exoenzymes and transcripts of pel-1, peh-1 and celVgenes than the HexA- and RpoS+ parent. Thus, the elevated levels of RpoS protein in the HexA- mutant do not account for exoenzyme overproduction. The following evidence associates for the first time the phenotypic changes in the HexA mutant to overproduction of rsmB RNA, a global regulator of exoenzymes, HarpinEcc, OHL and secondary metabolites. Analyses of rsmB transcripts and expression of an rsmB-lacZoperon fusion in E. c. carotovora strain Ecc71 revealed that HexA71 negatively regulates transcription of rsmB. Multiple copies of hexA71+ DNA suppress various phenotypes, including exoenzyme production in E. c. carotovora strain Ecc71, and concomitantly inhibit the production of rsmB, pel-1, peh-1, celV and hrpNEcc transcripts. Multiple copies of rsmB+ DNA, on the other hand, stimulate exoenzyme production by relieving the negative effects of a chromosomal copy of hexA+. The occurrence of hexA homologues and the negative effect of the dosage of hexA71 DNA on rsmB transcripts were also detected in other E. c. carotovora strains as well as Erwinia carotovora atroseptica and Erwinia carotovora betavasculorum. Extrapolating from the findings with LrhA, the Escherichia coli homologue of HexA, and the presence of sprE homologues in E. carotovora subspecies, we propose that HexA71 controls several regulatory pathways in E. carotovora including rsmB transcription and the production of SprEEcc which, in turn, affects RpoS levels. A model is presented that integrates the findings presented here and our current knowledge of the major regulatory network that controls exoprotein production in soft-rotting Erwinia carotovora subspecies. The soft-rotting bacterium, Erwinia carotovora ssp. carotovora (E. c. carotovora), produces an array of extracellular enzymes (= exoenzymes), including pectate lyase (Pel), polygalacturonase (Peh), cellulase (Cel) and protease (Prt), as well as HarpinEcc, the elicitor of hypersensitive reaction (HR). The production of these exoenzymes and HarpinEcc responds to plant products and the quorum-sensing signal [N-(3-oxohexanoyl)-L-homoserine lactone; OHL] and is subject to both transcriptional and post-transcriptional regulation. hexA of E. c. carotovora strain Ecc71 (hereafter hexA71), like that of another E. c. carotovora strain, negatively controls the production of exoenzymes, OHL and virulence in E. c. carotovora strain Ecc71. In addition to exoenzymes, HexA71 negatively regulates the expression of hrpNEcc, the structural gene for HarpinEcc. Exoenzyme overproduction is abolished by OHL deficiency in a HexA- and Ohll- double mutant, indicating that HexA and OHL are components of a common regulatory pathway controlling exoenzyme production. HexA71 negatively affects RpoS, as the levels of this alternative sigma factor are higher in the HexA- mutant than in the HexA+ strain. However, a HexA- and RpoS double mutant produces higher levels of exoenzymes and transcripts of pel-1, peh-1 and celVgenes than the HexA- and RpoS+ parent. Thus, the elevated levels of RpoS protein in the HexA- mutant do not account for exoenzyme overproduction. The following evidence associates for the first time the phenotypic changes in the HexA mutant to overproduction of rsmB RNA, a global regulator of exoenzymes, HarpinEcc, OHL and secondary metabolites. Analyses of rsmB transcripts and expression of an rsmB-lacZoperon fusion in E. c. carotovora strain Ecc71 revealed that HexA71 negatively regulates transcription of rsmB. Multiple copies of hexA71+ DNA suppress various phenotypes, including exoenzyme production in E. c. carotovora strain Ecc71, and concomitantly inhibit the production of rsmB, pel-1, peh-1, celV and hrpNEcc transcripts. Multiple copies of rsmB+ DNA, on the other hand, stimulate exoenzyme production by relieving the negative effects of a chromosomal copy of hexA+. The occurrence of hexA homologues and the negative effect of the dosage of hexA71 DNA on rsmB transcripts were also detected in other E. c. carotovora strains as well as Erwinia carotovora atroseptica and Erwinia carotovora betavasculorum. Extrapolating from the findings with LrhA, the Escherichia coli homologue of HexA, and the presence of sprE homologues in E. carotovora subspecies, we propose that HexA71 controls several regulatory pathways in E. carotovora including rsmB transcription and the production of SprEEcc which, in turn, affects RpoS levels. A model is presented that integrates the findings presented here and our current knowledge of the major regulatory network that controls exoprotein production in soft-rotting Erwinia carotovora subspecies. The soft‐rotting bacterium, Erwinia carotovora ssp. carotovora ( E. c. carotovora ), produces an array of extracellular enzymes (= exoenzymes), including pectate lyase (Pel), polygalacturonase (Peh), cellulase (Cel) and protease (Prt), as well as Harpin Ecc , the elicitor of hypersensitive reaction (HR). The production of these exoenzymes and Harpin Ecc responds to plant products and the quorum‐sensing signal [ N ‐(3‐oxohexanoyl)‐ l ‐homoserine lactone; OHL] and is subject to both transcriptional and post‐transcriptional regulation. hexA of E. c. carotovora strain Ecc71 (hereafter hexA 71 ), like that of another E. c. carotovora strain, negatively controls the production of exoenzymes, OHL and virulence in E. c. carotovora strain Ecc71. In addition to exoenzymes, HexA 71 negatively regulates the expression of hrpN Ecc , the structural gene for Harpin Ecc . Exoenzyme overproduction is abolished by OHL deficiency in a HexA − and OhlI − double mutant, indicating that HexA and OHL are components of a common regulatory pathway controlling exoenzyme production. HexA 71 negatively affects RpoS, as the levels of this alternative sigma factor are higher in the HexA − mutant than in the HexA + strain. However, a HexA − and RpoS − double mutant produces higher levels of exoenzymes and transcripts of pel‐1 , peh‐1 and celV genes than the HexA − and RpoS + parent. Thus, the elevated levels of RpoS protein in the HexA − mutant do not account for exoenzyme overproduction. The following evidence associates for the first time the phenotypic changes in the HexA − mutant to overproduction of rsmB RNA, a global regulator of exoenzymes, Harpin Ecc , OHL and secondary metabolites. Analyses of rsmB transcripts and expression of an rsmB–lacZ operon fusion in E. c. carotovora strain Ecc71 revealed that HexA 71 negatively regulates transcription of rsmB . Multiple copies of hexA 71 + DNA suppress various phenotypes, including exoenzyme production in E. c. carotovora strain Ecc71, and concomitantly inhibit the production of rsmB , pel‐1 , peh‐1 , celV and hrpN Ecc transcripts. Multiple copies of rsmB + DNA, on the other hand, stimulate exoenzyme production by relieving the negative effects of a chromosomal copy of hexA + . The occurrence of hexA homologues and the negative effect of the dosage of hexA 71 DNA on rsmB transcripts were also detected in other E. c. carotovora strains as well as Erwinia carotovora atroseptica and Erwinia carotovora betavasculorum . Extrapolating from the findings with LrhA, the Escherichia coli homologue of HexA, and the presence of sprE homologues in E. carotovora subspecies, we propose that HexA 71 controls several regulatory pathways in E. carotovora including rsmB transcription and the production of SprE Ecc which, in turn, affects RpoS levels. A model is presented that integrates the findings presented here and our current knowledge of the major regulatory network that controls exoprotein production in soft‐rotting Erwinia carotovora subspecies. The soft‐rotting bacterium, Erwinia carotovora ssp. carotovora (E. c. carotovora ), produces an array of extracellular enzymes (= exoenzymes), including pectate lyase (Pel), polygalacturonase (Peh), cellulase (Cel) and protease (Prt), as well as HarpinEcc, the elicitor of hypersensitive reaction (HR). The production of these exoenzymes and HarpinEcc responds to plant products and the quorum‐sensing signal [N‐(3‐oxohexanoyl)‐ l‐homoserine lactone; OHL] and is subject to both transcriptional and post‐transcriptional regulation. hexA of E. c. carotovora strain Ecc71 (hereafter hexA71), like that of another E. c. carotovora strain, negatively controls the production of exoenzymes, OHL and virulence in E. c. carotovora strain Ecc71. In addition to exoenzymes, HexA71 negatively regulates the expression of hrpNEcc, the structural gene for HarpinEcc. Exoenzyme overproduction is abolished by OHL deficiency in a HexA− and OhlI − double mutant, indicating that HexA and OHL are components of a common regulatory pathway controlling exoenzyme production. HexA71 negatively affects RpoS, as the levels of this alternative sigma factor are higher in the HexA− mutant than in the HexA+ strain. However, a HexA− and RpoS− double mutant produces higher levels of exoenzymes and transcripts of pel‐1, peh‐1 and celV genes than the HexA− and RpoS+ parent. Thus, the elevated levels of RpoS protein in the HexA− mutant do not account for exoenzyme overproduction. The following evidence associates for the first time the phenotypic changes in the HexA− mutant to overproduction of rsmB RNA, a global regulator of exoenzymes, HarpinEcc, OHL and secondary metabolites. Analyses of rsmB transcripts and expression of an rsmB–lacZ operon fusion in E. c. carotovora strain Ecc71 revealed that HexA71 negatively regulates transcription of rsmB. Multiple copies of hexA71+ DNA suppress various phenotypes, including exoenzyme production in E. c. carotovora strain Ecc71, and concomitantly inhibit the production of rsmB, pel‐1, peh‐1, celV and hrpNEcc transcripts. Multiple copies of rsmB+ DNA, on the other hand, stimulate exoenzyme production by relieving the negative effects of a chromosomal copy of hexA+. The occurrence of hexA homologues and the negative effect of the dosage of hexA71 DNA on rsmB transcripts were also detected in other E. c. carotovora strains as well as Erwinia carotovora atroseptica and Erwinia carotovora betavasculorum. Extrapolating from the findings with LrhA, the Escherichia coli homologue of HexA, and the presence of sprE homologues in E. carotovora subspecies, we propose that HexA71 controls several regulatory pathways in E. carotovora including rsmB transcription and the production of SprEEcc which, in turn, affects RpoS levels. A model is presented that integrates the findings presented here and our current knowledge of the major regulatory network that controls exoprotein production in soft‐rotting Erwinia carotovora subspecies. |
| Author | Mukherjee, Asita Cui, Yaya Liu, Yang Ma, Weilei Chatterjee, Arun K. |
| Author_xml | – sequence: 1 givenname: Asita surname: Mukherjee fullname: Mukherjee, Asita organization: Department of Plant Microbiology and Pathology, Plant Sciences Unit, 108 Waters Hall, University of Missouri, Columbia, MO 65211, USA – sequence: 2 givenname: Yaya surname: Cui fullname: Cui, Yaya organization: Department of Plant Microbiology and Pathology, Plant Sciences Unit, 108 Waters Hall, University of Missouri, Columbia, MO 65211, USA – sequence: 3 givenname: Weilei surname: Ma fullname: Ma, Weilei organization: Department of Plant Microbiology and Pathology, Plant Sciences Unit, 108 Waters Hall, University of Missouri, Columbia, MO 65211, USA – sequence: 4 givenname: Yang surname: Liu fullname: Liu, Yang organization: Department of Plant Microbiology and Pathology, Plant Sciences Unit, 108 Waters Hall, University of Missouri, Columbia, MO 65211, USA – sequence: 5 givenname: Arun K. surname: Chatterjee fullname: Chatterjee, Arun K. organization: Department of Plant Microbiology and Pathology, Plant Sciences Unit, 108 Waters Hall, University of Missouri, Columbia, MO 65211, USA |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/11220306$$D View this record in MEDLINE/PubMed |
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| ContentType | Journal Article |
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| DOI | 10.1046/j.1462-2920.2000.00093.x |
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| EndPage | 215 |
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| PublicationTitle | Environmental microbiology |
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| PublicationYear | 2000 |
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| Snippet | The soft‐rotting bacterium, Erwinia carotovora ssp. carotovora (E. c. carotovora ), produces an array of extracellular enzymes (= exoenzymes), including... The soft‐rotting bacterium, Erwinia carotovora ssp. carotovora ( E. c. carotovora ), produces an array of extracellular enzymes (= exoenzymes), including... The soft-rotting bacterium, Erwinia carotovora ssp. carotovora (E. c. carotovora), produces an array of extracellular enzymes (= exoenzymes), including pectate... |
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| SubjectTerms | 4-Butyrolactone - analogs & derivatives 4-Butyrolactone - analysis 4-Butyrolactone - genetics Bacterial Outer Membrane Proteins - genetics Bacterial Proteins - analysis Bacterial Proteins - genetics Cellulase - genetics DNA-Binding Proteins Escherichia coli Proteins Gene Expression Regulation Lac Operon Mutation Operon Pectobacterium carotovorum - genetics Pectobacterium carotovorum - pathogenicity Plant Diseases - microbiology Polygalacturonase - analysis Polygalacturonase - genetics Polysaccharide-Lyases - analysis Polysaccharide-Lyases - genetics Repressor Proteins - analysis Repressor Proteins - genetics RNA, Bacterial - analysis RNA, Messenger - analysis Sigma Factor - analysis Sigma Factor - genetics Transcription Factors Virulence |
| Title | hexA of Erwinia carotovora ssp. carotovora strain Ecc71 negatively regulates production of RpoS and rsmB RNA, a global regulator of extracellular proteins, plant virulence and the quorum-sensing signal, N-(3-oxohexanoyl)-l-homoserine lactone |
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