Alginate hydrogel matrix stiffness influences the in vitro development of caprine preantral follicles

• Published in: Molecular reproduction and development Vol. 81; no. 7; pp. 636 - 645
• Main Authors: Brito, I.R., Silva, C.M.G., Duarte, A.B.G., Lima, I.M.T., Rodrigues, G.Q., Rossetto, R., Sales, A.D., Lobo, C.H., Bernuci, M.P., Rosa-e-Silva, A.C.J.S., Campello, C.C., Xu, M., Figueiredo, J.R.
• Format: Journal Article
• Language: English
• Published: United States Blackwell Publishing Ltd 01.07.2014; Wiley Subscription Services, Inc
• Subjects: 3-Hydroxysteroid Dehydrogenases - analysis; 3-Hydroxysteroid Dehydrogenases - genetics; 3-Hydroxysteroid Dehydrogenases - metabolism; Alginates - chemistry; Alginates - pharmacology; Animals; Aromatase - analysis; Aromatase - genetics; Aromatase - metabolism; Cell Culture Techniques; Female; Goats; Hydrogel, Polyethylene Glycol Dimethacrylate - chemistry; Hydrogel, Polyethylene Glycol Dimethacrylate - pharmacology; Oocytes - drug effects; Oocytes - growth & development; Ovarian Follicle - drug effects; Ovarian Follicle - growth & development

SUMMARY This study examined caprine follicular development in different concentrations of alginate matrix to determine the optimal conditions for culture. Caprine preantral follicles were cultured in a two‐dimensional system (control) or a three‐dimensional encapsulated system in 0.25%, 0.5%, or 1%...