Combining 2-DE immunoblots and mass spectrometry to identify putative soybean (Glycine max) allergens

• Published in: Food and chemical toxicology Vol. 116; no. Pt B; pp. 207 - 215
• Main Authors: Lu, Mei, Jin, Yuan, Cerny, Ron, Ballmer-Weber, Barbara, Goodman, Richard E.
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.06.2018
• Subjects: agglutinins; allergenicity; allergens; Allergens - blood; Antigens, Plant - blood; beta-conglycinin; binding proteins; Blotting, Western - methods; Chromatography, Liquid; embryogenesis; Food Hypersensitivity - immunology; gels; genetic engineering; Globulins; Glycine max; Glycine max - immunology; Glycinin; Humans; IgE; immunoglobulin E; Immunoglobulin E - metabolism; Kunitz-type proteinase inhibitor; LC-MS/MS; mass spectrometry; Mass Spectrometry - methods; quantitative analysis; risk assessment; Seed Storage Proteins - blood; soy protein; Soybean allergens; Soybean Proteins - blood; soybeans; spectrometers; Tandem Mass Spectrometry; toxicology; Trypsin Inhibitor, Kunitz Soybean - metabolism; Two-dimensional immunoblots; β-conglycinin; SBP; MOWSE; IgE; Two-dimensional immunoblots; WHO/IUIS; Glycinin; MW; SKTI; CHAPS; β-conglycinin; LC-MS/MS; OECD; vdc; LEP; SPT; NCBI; DBPCFC; GE; Soybean allergens

Soybean is recognized as a commonly allergenic food, but the identity of important allergens is not well studied. Recently, some global regulatory agencies started requiring quantitative analysis of individual allergens, including unproven allergens, as part of the risk assessment for genetically en...