Cordycepin-hypersensitive growth links elevated polyphosphate levels to inhibition of poly(A) polymerase in Saccharomyces cerevisiae

• Published in: Nucleic acids research Vol. 36; no. 2; pp. 353 - 363
• Main Authors: Holbein, Sandra, Freimoser, Florian M, Werner, Thomas P, Wengi, Agnieszka, Dichtl, Bernhard
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 01.02.2008; Oxford Publishing Limited (England)
• Subjects: Cyclin-Dependent Kinases - genetics; Cyclins - genetics; Deoxyadenosines - pharmacology; Gene Deletion; Nucleic Acid Enzymes; Polyadenylation; Polynucleotide Adenylyltransferase - antagonists & inhibitors; Polynucleotide Adenylyltransferase - metabolism; Polyphosphates - metabolism; Repressor Proteins - genetics; Saccharomyces cerevisiae - enzymology; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - growth & development; Saccharomyces cerevisiae Proteins - genetics

To identify genes involved in poly(A) metabolism, we screened the yeast gene deletion collection for growth defects in the presence of cordycepin (3'-deoxyadenosine), a precursor to the RNA chain terminating ATP analog cordycepin triphosphate. Δpho80 and Δpho85 strains, which have a constitutiv...