Development of Dembo-PCR for cattle in accordance with Japanese livestock quarantine guidelines

• Published in: Journal of Veterinary Medical Science Vol. 87; no. 1; pp. 80 - 85
• Main Authors: OBA, Mami, IMAI, Ryo, TESHIMA, Natsuko, NODA, Miho, NUNOMURA, Yuka, KAKINUMA, Seiichi, ITO, Kazuki, OGURO, Masashi, KONAKA, Kazunari, ENOMOTO, Tomoya, MORITA, Hisamoto, OMATSU, Tsutomu, NAGAI, Makoto, MIZUTANI, Tetsuya
• Format: Journal Article
• Language: English
• Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 01.01.2025; Japan Science and Technology Agency; The Japanese Society of Veterinary Science
• Subjects: Animals; Cattle; Cattle Diseases - diagnosis; Cattle Diseases - microbiology; comprehensive; Dembo-PCR; Infectious diseases; Japan; Laboratory Animal Science; Livestock; pathogen; Pathogens; Quarantine; Quarantine - veterinary; real-time PCR; Real-Time Polymerase Chain Reaction - methods; Real-Time Polymerase Chain Reaction - veterinary; Japan; pathogen; cattle; Dembo-PCR; real-time PCR; comprehensive

Detection of microorganisms from bovine using real-time PCR (Dembo-PCR) is a comprehensive detection technique that was developed to detect pathogens causing bovine diseases. In Japan, the definitive tests for monitored infectious diseases, which are defined by law, are carried out at government age...