Development of Dembo-PCR for cattle in accordance with Japanese livestock quarantine guidelines
Detection of microorganisms from bovine using real-time PCR (Dembo-PCR) is a comprehensive detection technique that was developed to detect pathogens causing bovine diseases. In Japan, the definitive tests for monitored infectious diseases, which are defined by law, are carried out at government age...
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| Published in | Journal of Veterinary Medical Science Vol. 87; no. 1; pp. 80 - 85 |
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| Main Authors | , , , , , , , , , , , , , |
| Format | Journal Article |
| Language | English |
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Japan
JAPANESE SOCIETY OF VETERINARY SCIENCE
01.01.2025
Japan Science and Technology Agency The Japanese Society of Veterinary Science |
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| Abstract | Detection of microorganisms from bovine using real-time PCR (Dembo-PCR) is a comprehensive detection technique that was developed to detect pathogens causing bovine diseases. In Japan, the definitive tests for monitored infectious diseases, which are defined by law, are carried out at government agencies. On the other hand, the existence of pathogens other than monitored infectious diseases are not well understood. From the perspective of livestock quarantine, it is important to make it possible to identify pathogens other than monitored infectious diseases, so we extended the existing Dembo-PCR system in this study. In particular, the number of targets other than monitored infectious disease was increased. The new version of Dembo-PCR may be useful in elucidating new pathologies associated with multiple pathogens. |
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| AbstractList | Detection of microorganisms from bovine using real-time PCR (Dembo-PCR) is a comprehensive detection technique that was developed to detect pathogens causing bovine diseases. In Japan, the definitive tests for monitored infectious diseases, which are defined by law, are carried out at government agencies. On the other hand, the existence of pathogens other than monitored infectious diseases are not well understood. From the perspective of livestock quarantine, it is important to make it possible to identify pathogens other than monitored infectious diseases, so we extended the existing Dembo-PCR system in this study. In particular, the number of targets other than monitored infectious disease was increased. The new version of Dembo-PCR may be useful in elucidating new pathologies associated with multiple pathogens. Detection of microorganisms from bovine using real-time PCR (Dembo-PCR) is a comprehensive detection technique that was developed to detect pathogens causing bovine diseases. In Japan, the definitive tests for monitored infectious diseases, which are defined by law, are carried out at government agencies. On the other hand, the existence of pathogens other than monitored infectious diseases are not well understood. From the perspective of livestock quarantine, it is important to make it possible to identify pathogens other than monitored infectious diseases, so we extended the existing Dembo-PCR system in this study. In particular, the number of targets other than monitored infectious disease was increased. The new version of Dembo-PCR may be useful in elucidating new pathologies associated with multiple pathogens. Detection of microorganisms from bovine using real-time PCR (Dembo PCR) is a comprehensive detection technique that was developed to detect pathogens causing bovine diseases. In Japan, the definitive tests for monitored infectious diseases, which are defined by law, are carried out at government agencies. On the other hand, the existence of pathogens other than monitored infectious diseases are not well understood. From the perspective of livestock quarantine, it is important to make it possible to identify pathogens other than monitored infectious diseases, so we extended the existing Dembo PCR system in this study. In particular, the number of targets other than monitored infectious disease was increased. The new version of Dembo PCR may be useful in elucidating new pathologies associated with multiple pathogens.Detection of microorganisms from bovine using real-time PCR (Dembo PCR) is a comprehensive detection technique that was developed to detect pathogens causing bovine diseases. In Japan, the definitive tests for monitored infectious diseases, which are defined by law, are carried out at government agencies. On the other hand, the existence of pathogens other than monitored infectious diseases are not well understood. From the perspective of livestock quarantine, it is important to make it possible to identify pathogens other than monitored infectious diseases, so we extended the existing Dembo PCR system in this study. In particular, the number of targets other than monitored infectious disease was increased. The new version of Dembo PCR may be useful in elucidating new pathologies associated with multiple pathogens. |
| ArticleNumber | 24-0030 |
| Author | KONAKA, Kazunari KAKINUMA, Seiichi OGURO, Masashi NAGAI, Makoto IMAI, Ryo OMATSU, Tsutomu NUNOMURA, Yuka MIZUTANI, Tetsuya OBA, Mami ITO, Kazuki NODA, Miho MORITA, Hisamoto TESHIMA, Natsuko ENOMOTO, Tomoya |
| Author_xml | – sequence: 1 fullname: OBA, Mami organization: Center for infectious Disease Epidemiology and Prevention Research, Tokyo University of Agriculture and Technology, Tokyo, Japan – sequence: 2 fullname: IMAI, Ryo organization: Center for infectious Disease Epidemiology and Prevention Research, Tokyo University of Agriculture and Technology, Tokyo, Japan – sequence: 3 fullname: TESHIMA, Natsuko organization: Center for infectious Disease Epidemiology and Prevention Research, Tokyo University of Agriculture and Technology, Tokyo, Japan – sequence: 4 fullname: NODA, Miho organization: Center for infectious Disease Epidemiology and Prevention Research, Tokyo University of Agriculture and Technology, Tokyo, Japan – sequence: 5 fullname: NUNOMURA, Yuka organization: Center for infectious Disease Epidemiology and Prevention Research, Tokyo University of Agriculture and Technology, Tokyo, Japan – sequence: 6 fullname: KAKINUMA, Seiichi organization: Kakinuma Veterinary Hospital, Saitama, Japan – sequence: 7 fullname: ITO, Kazuki organization: Veterinary Clinic, Saitama Agricultural Mutual Aid Association, Saitama, Japan – sequence: 8 fullname: OGURO, Masashi organization: MYDS Corporation, Gunma, Japan – sequence: 9 fullname: KONAKA, Kazunari organization: KM Clinic, Tochigi, Japan – sequence: 10 fullname: ENOMOTO, Tomoya organization: Veterinary Clinic, Kanagawa Agricultural Mutual Aid Association, Kanagawa, Japan – sequence: 11 fullname: MORITA, Hisamoto organization: Morita Veterinary Hospital, Kanagawa, Japan – sequence: 12 fullname: OMATSU, Tsutomu organization: Center for infectious Disease Epidemiology and Prevention Research, Tokyo University of Agriculture and Technology, Tokyo, Japan – sequence: 13 fullname: NAGAI, Makoto organization: School of Veterinary Medicine, Azabu University, Kanagawa, Japan – sequence: 14 fullname: MIZUTANI, Tetsuya organization: Center for infectious Disease Epidemiology and Prevention Research, Tokyo University of Agriculture and Technology, Tokyo, Japan |
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| References | 11. Lew AE, Bock RE, Miles J, Cuttell LB, Steer P, Nadin-Davis SA. 2004. Sensitive and specific detection of bovine immunodeficiency virus and bovine syncytial virus by 5′ Taq nuclease assays with fluorescent 3′ minor groove binder-DNA probes. J Virol Methods 116: 1–9. 17. Rahpaya SS, Tsuchiaka S, Kishimoto M, Oba M, Katayama Y, Nunomura Y, Kokawa S, Kimura T, Kobayashi A, Kirino Y, Okabayashi T, Nonaka N, Mekata H, Aoki H, Shiokawa M, Umetsu M, Morita T, Hasebe A, Otsu K, Asai T, Yamaguchi T, Makino S, Murata Y, Abi AJ, Omatsu T, Mizutani T. 2018. Dembo polymerase chain reaction technique for detection of bovine abortion, diarrhea, and respiratory disease complex infectious agents in potential vectors and reservoirs. J Vet Sci 19: 350–357. 22. Zheng W, Porter E, Noll L, Stoy C, Lu N, Wang Y, Liu X, Purvis T, Peddireddi L, Lubbers B, Hanzlicek G, Henningson J, Liu Z, Bai J. 2019. A multiplex real-time PCR assay for the detection and differentiation of five bovine pinkeye pathogens. J Microbiol Methods 160: 87–92. 5. Burnet JB, Ogorzaly L, Tissier A, Penny C, Cauchie HM. 2013. Novel quantitative TaqMan real-time PCR assays for detection of Cryptosporidium at the genus level and genotyping of major human and cattle-infecting species. J Appl Microbiol 114: 1211–1222. 13. Ministry of Agriculture Forestry and Fisheries (MAFF). 2020. Act on the Prevention of Infectious Diseases in Livestock. https://www.japaneselawtranslation.go.jp/ja/laws/view/4114 [accessed on Jan 20, 2024]. 16. Ouedraogo A, Luciani L, Zannou O, Biguezoton A, Pezzi L, Thirion L, Belem A, Saegerman C, Charrel R, Lempereur L. 2020. Detection of two species of the genus Parapoxvirus (bovine papular stomatitis virus and pseudocowpox virus) in ticks infesting cattle in Burkina Faso. Microorganisms 8: 8. 18. Schindler AR, Vögtlin A, Hilbe M, Puorger M, Zlinszky K, Ackermann M, Ehrensperger F. 2007. Reverse transcription real-time PCR assays for detection and quantification of Borna disease virus in diseased hosts. Mol Cell Probes 21: 47–55. 21. United States Environmental Protection Agency. 2014. EPA technology for mold identification and enumeration. https://irp-cdn.multiscreensite.com/c4e267ab/files/uploaded/gCQnkBNWQuSD96fPIikY_EPA_Technology%20for%20Mold%20Identification%20and%20Enumeration.pdf [accessed on March 23, 2021]. 10. Kishimoto M, Tsuchiaka S, Rahpaya SS, Hasebe A, Otsu K, Sugimura S, Kobayashi S, Komatsu N, Nagai M, Omatsu T, Naoi Y, Sano K, Okazaki-Terashima S, Oba M, Katayama Y, Sato R, Asai T, Mizutani T. 2017. Development of a one-run real-time PCR detection system for pathogens associated with bovine respiratory disease complex. J Vet Med Sci 79: 517–523. 14. Oba M, Obinata S, Takemae H, Kazama K, Oguro M, Ito K, Kakinuma S, Ishida H, Murakami H, Sakaguchi S, Mizutani T, Nagai M. 2023. Prevalence and genetic diversity in bovine parechovirus infecting Japanese cattle. Arch Virol 168: 91. 19. Suo B, He Y, Tu SI, Shi X. 2010. A multiplex real-time polymerase chain reaction for simultaneous detection of Salmonella spp., Escherichia coli O157, and Listeria monocytogenes in meat products. Foodborne Pathog Dis 7: 619–628. 3. Bergmans AM, van der Ent M, Klaassen A, Böhm N, Andriesse GI, Wintermans RG. 2010. Evaluation of a single-tube real-time PCR for detection and identification of 11 dermatophyte species in clinical material. Clin Microbiol Infect 16: 704–710. 7. Decaro N, Carelli G, Lorusso E, Lucente MS, Greco G, Lorusso A, Radogna A, Ceci L, Buonavoglia C. 2008. Duplex real-time polymerase chain reaction for simultaneous detection and quantification of Anaplasma marginale and Anaplasma centrale. J Vet Diagn Invest 20: 606–611. 9. Kawasaki J, Kojima S, Tomonaga K, Horie M. 2021. Hidden viral sequences in public sequencing data and warning for future emerging diseases. MBio 12: e0163821. 12. Maksyutov RA, Gavrilova EV, Meyer H, Shchelkunov SN. 2015. Real-time PCR assay for specific detection of cowpox virus. J Virol Methods 211: 8–11. 20. Tsuchiaka S, Masuda T, Sugimura S, Kobayashi S, Komatsu N, Nagai M, Omatsu T, Furuya T, Oba M, Katayama Y, Kanda S, Yokoyama T, Mizutani T. 2016. Development of a novel detection system for microbes from bovine diarrhea by real-time PCR. J Vet Med Sci 78: 383–389. 1. Ade J, Niethammer F, Schade B, Schilling T, Hoelzle K, Hoelzle LE. 2018. Quantitative analysis of Mycoplasma wenyonii and ‘Candidatus Mycoplasma haemobos” infections in cattle using novel gapN-based realtime PCR assays. Vet Microbiol 220: 1–6. 8. Gomez A, Cook NB, Bernardoni ND, Rieman J, Dusick AF, Hartshorn R, Socha MT, Read DH, Döpfer D. 2012. An experimental infection model to induce digital dermatitis infection in cattle. J Dairy Sci 95: 1821–1830. 15. Oba M, Sakaguchi S, Wu H, Fujioka Y, Takemae H, Oki H, Kawai M, Shiokawa M, Aoki H, Fukase Y, Madarame H, Nakano T, Mizutani T, Nagai M. 2022. First isolation and genomic characterization of bovine parechovirus from faecal samples of cattle in Japan. J Gen Virol 103: 103. 2. Bašková L, Landlinger C, Preuner S, Lion T. 2007. The Pan-AC assay: a single-reaction real-time PCR test for quantitative detection of a broad range of Aspergillus and Candida species. J Med Microbiol 56: 1167–1173. 4. Bogaert L, Van Poucke M, De Baere C, Dewulf J, Peelman L, Ducatelle R, Gasthuys F, Martens A. 2007. Bovine papillomavirus load and mRNA expression, cell proliferation and p53 expression in four clinical types of equine sarcoid. J Gen Virol 88: 2155–2161. 6. de Bruin A, de Groot A, de Heer L, Bok J, Wielinga PR, Hamans M, van Rotterdam BJ, Janse I. 2011. Detection of Coxiella burnetii in complex matrices by using multiplex quantitative PCR during a major Q fever outbreak in The Netherlands. Appl Environ Microbiol 77: 6516–6523. 11 22 12 13 14 15 16 17 18 19 1 2 3 4 5 6 7 8 9 20 10 21 |
| References_xml | – reference: 16. Ouedraogo A, Luciani L, Zannou O, Biguezoton A, Pezzi L, Thirion L, Belem A, Saegerman C, Charrel R, Lempereur L. 2020. Detection of two species of the genus Parapoxvirus (bovine papular stomatitis virus and pseudocowpox virus) in ticks infesting cattle in Burkina Faso. Microorganisms 8: 8. – reference: 22. Zheng W, Porter E, Noll L, Stoy C, Lu N, Wang Y, Liu X, Purvis T, Peddireddi L, Lubbers B, Hanzlicek G, Henningson J, Liu Z, Bai J. 2019. A multiplex real-time PCR assay for the detection and differentiation of five bovine pinkeye pathogens. J Microbiol Methods 160: 87–92. – reference: 12. Maksyutov RA, Gavrilova EV, Meyer H, Shchelkunov SN. 2015. Real-time PCR assay for specific detection of cowpox virus. J Virol Methods 211: 8–11. – reference: 13. Ministry of Agriculture Forestry and Fisheries (MAFF). 2020. Act on the Prevention of Infectious Diseases in Livestock. https://www.japaneselawtranslation.go.jp/ja/laws/view/4114 [accessed on Jan 20, 2024]. – reference: 18. Schindler AR, Vögtlin A, Hilbe M, Puorger M, Zlinszky K, Ackermann M, Ehrensperger F. 2007. Reverse transcription real-time PCR assays for detection and quantification of Borna disease virus in diseased hosts. Mol Cell Probes 21: 47–55. – reference: 14. Oba M, Obinata S, Takemae H, Kazama K, Oguro M, Ito K, Kakinuma S, Ishida H, Murakami H, Sakaguchi S, Mizutani T, Nagai M. 2023. Prevalence and genetic diversity in bovine parechovirus infecting Japanese cattle. Arch Virol 168: 91. – reference: 10. Kishimoto M, Tsuchiaka S, Rahpaya SS, Hasebe A, Otsu K, Sugimura S, Kobayashi S, Komatsu N, Nagai M, Omatsu T, Naoi Y, Sano K, Okazaki-Terashima S, Oba M, Katayama Y, Sato R, Asai T, Mizutani T. 2017. Development of a one-run real-time PCR detection system for pathogens associated with bovine respiratory disease complex. J Vet Med Sci 79: 517–523. – reference: 9. Kawasaki J, Kojima S, Tomonaga K, Horie M. 2021. Hidden viral sequences in public sequencing data and warning for future emerging diseases. MBio 12: e0163821. – reference: 3. Bergmans AM, van der Ent M, Klaassen A, Böhm N, Andriesse GI, Wintermans RG. 2010. Evaluation of a single-tube real-time PCR for detection and identification of 11 dermatophyte species in clinical material. Clin Microbiol Infect 16: 704–710. – reference: 2. Bašková L, Landlinger C, Preuner S, Lion T. 2007. The Pan-AC assay: a single-reaction real-time PCR test for quantitative detection of a broad range of Aspergillus and Candida species. J Med Microbiol 56: 1167–1173. – reference: 7. Decaro N, Carelli G, Lorusso E, Lucente MS, Greco G, Lorusso A, Radogna A, Ceci L, Buonavoglia C. 2008. Duplex real-time polymerase chain reaction for simultaneous detection and quantification of Anaplasma marginale and Anaplasma centrale. J Vet Diagn Invest 20: 606–611. – reference: 19. Suo B, He Y, Tu SI, Shi X. 2010. A multiplex real-time polymerase chain reaction for simultaneous detection of Salmonella spp., Escherichia coli O157, and Listeria monocytogenes in meat products. Foodborne Pathog Dis 7: 619–628. – reference: 17. Rahpaya SS, Tsuchiaka S, Kishimoto M, Oba M, Katayama Y, Nunomura Y, Kokawa S, Kimura T, Kobayashi A, Kirino Y, Okabayashi T, Nonaka N, Mekata H, Aoki H, Shiokawa M, Umetsu M, Morita T, Hasebe A, Otsu K, Asai T, Yamaguchi T, Makino S, Murata Y, Abi AJ, Omatsu T, Mizutani T. 2018. Dembo polymerase chain reaction technique for detection of bovine abortion, diarrhea, and respiratory disease complex infectious agents in potential vectors and reservoirs. J Vet Sci 19: 350–357. – reference: 20. Tsuchiaka S, Masuda T, Sugimura S, Kobayashi S, Komatsu N, Nagai M, Omatsu T, Furuya T, Oba M, Katayama Y, Kanda S, Yokoyama T, Mizutani T. 2016. Development of a novel detection system for microbes from bovine diarrhea by real-time PCR. J Vet Med Sci 78: 383–389. – reference: 5. Burnet JB, Ogorzaly L, Tissier A, Penny C, Cauchie HM. 2013. Novel quantitative TaqMan real-time PCR assays for detection of Cryptosporidium at the genus level and genotyping of major human and cattle-infecting species. J Appl Microbiol 114: 1211–1222. – reference: 6. de Bruin A, de Groot A, de Heer L, Bok J, Wielinga PR, Hamans M, van Rotterdam BJ, Janse I. 2011. Detection of Coxiella burnetii in complex matrices by using multiplex quantitative PCR during a major Q fever outbreak in The Netherlands. Appl Environ Microbiol 77: 6516–6523. – reference: 11. Lew AE, Bock RE, Miles J, Cuttell LB, Steer P, Nadin-Davis SA. 2004. Sensitive and specific detection of bovine immunodeficiency virus and bovine syncytial virus by 5′ Taq nuclease assays with fluorescent 3′ minor groove binder-DNA probes. J Virol Methods 116: 1–9. – reference: 1. Ade J, Niethammer F, Schade B, Schilling T, Hoelzle K, Hoelzle LE. 2018. Quantitative analysis of Mycoplasma wenyonii and ‘Candidatus Mycoplasma haemobos” infections in cattle using novel gapN-based realtime PCR assays. Vet Microbiol 220: 1–6. – reference: 8. Gomez A, Cook NB, Bernardoni ND, Rieman J, Dusick AF, Hartshorn R, Socha MT, Read DH, Döpfer D. 2012. An experimental infection model to induce digital dermatitis infection in cattle. J Dairy Sci 95: 1821–1830. – reference: 21. United States Environmental Protection Agency. 2014. EPA technology for mold identification and enumeration. https://irp-cdn.multiscreensite.com/c4e267ab/files/uploaded/gCQnkBNWQuSD96fPIikY_EPA_Technology%20for%20Mold%20Identification%20and%20Enumeration.pdf [accessed on March 23, 2021]. – reference: 4. Bogaert L, Van Poucke M, De Baere C, Dewulf J, Peelman L, Ducatelle R, Gasthuys F, Martens A. 2007. Bovine papillomavirus load and mRNA expression, cell proliferation and p53 expression in four clinical types of equine sarcoid. J Gen Virol 88: 2155–2161. – reference: 15. Oba M, Sakaguchi S, Wu H, Fujioka Y, Takemae H, Oki H, Kawai M, Shiokawa M, Aoki H, Fukase Y, Madarame H, Nakano T, Mizutani T, Nagai M. 2022. First isolation and genomic characterization of bovine parechovirus from faecal samples of cattle in Japan. J Gen Virol 103: 103. – ident: 1 doi: 10.1016/j.vetmic.2018.04.028 – ident: 10 doi: 10.1292/jvms.16-0489 – ident: 5 doi: 10.1111/jam.12103 – ident: 11 doi: 10.1016/j.jviromet.2003.10.006 – ident: 18 doi: 10.1016/j.mcp.2006.08.001 – ident: 6 doi: 10.1128/AEM.05097-11 – ident: 12 doi: 10.1016/j.jviromet.2014.10.004 – ident: 9 doi: 10.1128/mBio.01638-21 – ident: 17 doi: 10.4142/jvs.2018.19.3.350 – ident: 4 doi: 10.1099/vir.0.82876-0 – ident: 8 doi: 10.3168/jds.2011-4754 – ident: 13 – ident: 2 doi: 10.1099/jmm.0.47212-0 – ident: 19 doi: 10.1089/fpd.2009.0430 – ident: 20 doi: 10.1292/jvms.15-0552 – ident: 16 doi: 10.3390/microorganisms8050644 – ident: 15 doi: 10.1099/jgv.0.001718 – ident: 22 doi: 10.1016/j.mimet.2019.03.024 – ident: 7 doi: 10.1177/104063870802000511 – ident: 21 – ident: 14 doi: 10.1007/s00705-023-05712-x – ident: 3 doi: 10.1111/j.1469-0691.2009.02991.x |
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| SubjectTerms | Animals Cattle Cattle Diseases - diagnosis Cattle Diseases - microbiology comprehensive Dembo-PCR Infectious diseases Japan Laboratory Animal Science Livestock pathogen Pathogens Quarantine Quarantine - veterinary real-time PCR Real-Time Polymerase Chain Reaction - methods Real-Time Polymerase Chain Reaction - veterinary |
| Title | Development of Dembo-PCR for cattle in accordance with Japanese livestock quarantine guidelines |
| URI | https://www.jstage.jst.go.jp/article/jvms/87/1/87_24-0030/_article/-char/en https://www.ncbi.nlm.nih.gov/pubmed/39581586 https://www.proquest.com/docview/3166956145 https://www.proquest.com/docview/3132608848 https://pubmed.ncbi.nlm.nih.gov/PMC11735209 |
| Volume | 87 |
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| ispartofPNX | Journal of Veterinary Medical Science, 2025, Vol.87(1), pp.80-85 |
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