TALE nickase-mediated SP110 knockin endows cattle with increased resistance to tuberculosis

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 112; no. 13; pp. E1530 - E1539
• Main Authors: Wu, Haibo, Wang, Yongsheng, Zhang, Yan, Yang, Mingqi, Lv, Jiaxing, Liu, Jun, Zhang, Yong
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 31.03.2015; National Acad Sciences
• Series: PNAS Plus
• Subjects: animal breeding; Animals; Apoptosis; Bacteria; Biological Sciences; bovine tuberculosis; Cattle; Deoxyribonuclease I - metabolism; disease resistance; DNA Breaks, Single-Stranded; Endonucleases - metabolism; Enzyme-Linked Immunospot Assay; Experiments; Female; Gene Knock-In Techniques; Gene Transfer Techniques; genes; Genome; Genomes; HEK293 Cells; hosts; Humans; Macrophages - microbiology; Mice; Minor Histocompatibility Antigens; mutation; Mycobacterium bovis; Nuclear Proteins - genetics; Nuclear Proteins - physiology; PNAS Plus; transcription (genetics); Transcription factors; Transcription, Genetic; Transgenic animals; Tuberculosis; Tuberculosis, Bovine - genetics; Tuberculosis, Bovine - microbiology; Tuberculosis, Bovine - prevention & control; disease resistance; homologous recombination; TALEN; single-strand break; tuberculosis
• ISSN: 0027-8424; 1091-6490; 1091-6490
• DOI: 10.1073/pnas.1421587112

Transcription activator-like effector nuclease (TALEN)-mediated genome modification has been applied successfully to create transgenic animals in various species, such as mouse, pig, and even monkey. However, transgenic cattle with gene knockin have yet to be created using TALENs. Here, we report si...