Role of phenazines and cyclic lipopeptides produced by pseudomonas sp. CMR12a in induced systemic resistance on rice and bean

• Published in: Environmental microbiology reports Vol. 8; no. 5; pp. 896 - 904
• Main Authors: Ma, Zongwang, Hua, Gia Khuong Hoang, Ongena, Marc, Höfte, Monica
• Format: Journal Article Web Resource
• Language: English
• Published: United States Blackwell Publishing Ltd 01.10.2016; John Wiley & Sons, Inc; Wiley-Blackwell
• Subjects: Antagonism; Bacteria; Beans; Biochemistry, biophysics & molecular biology; Biochimie, biophysique & biologie moléculaire; Biosynthesis; Blight; Disease resistance; Experiments; Leaves; Life sciences; Lipopeptides; Metabolites; Microorganisms; Mutants; Pathogens; Phenazine; Plant diseases; Plant resistance; Pseudomonas; Rice blast; Sciences du vivant; Soil microorganisms; Soil resistance; Web blight; Webs
• DOI: 10.1111/1758-2229.12454
• ISSN: 1758-2229

Summary Pseudomonas sp. CMR12a produces two different classes of cyclic lipopeptides (CLPs) (orfamides and sessilins), which all play a role in direct antagonism against soilborne pathogens. Here we show that Pseudomonas sp. CMR12a is also able to induce systemic resistance to Magnaporthe oryzae on rice and to the web blight pathogen Rhizoctonia solani AG2‐2 on bean. Plant assays with biosynthesis mutants of Pseudomonas sp. CMR12a impaired in the production of phenazines and/or CLPs and purified metabolites revealed that distinct bacterial determinants are responsible for inducing systemic resistance in these two pathosystems. In rice, mutants impaired in phenazine production completely lost their ability to induce systemic resistance, while a soil drench with pure phenazine‐1‐carboxamide (PCN) at a concentration of 0.1 or 1 μM was active in inducing resistance against M. oryzae. In bean, mutants that only produced phenazines, sessilins or orfamides were still able to induce systemic resistance against Rhizoctonia web blight, but a balanced production of these metabolites was needed. This study not only shows that Pseudomonas sp. CMR12a can protect rice to blast disease and bean to web blight disease, but also displays that the determinants involved in induced systemic resistance are plant, pathogen and concentration dependent.