Ultrasensitive in vivo infrared spectroscopic imaging via oblique photothermal microscopy

• Published in: Nature communications Vol. 16; no. 1; pp. 6065 - 15
• Main Authors: Li, Mingsheng, Xiao, Sheng, Ni, Hongli, Ding, Guangrui, Yuan, Yuhao, Marar, Carolyn, Mertz, Jerome, Cheng, Ji-Xin
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 02.07.2025; Nature Publishing Group; Nature Portfolio
• Subjects: 140/125; 631/1647/245/2226; 639/624/1107/510; 639/624/1107/527/2257; 9/10; Animals; Efficiency; Female; Humanities and Social Sciences; Humans; Infrared analysis; Infrared imaging; Infrared spectroscopy; Lasers; Mice; Microscopy; Microscopy - instrumentation; Microscopy - methods; Monte Carlo simulation; multidisciplinary; Photons; Propagation; Science; Science (multidisciplinary); Sensitivity; Sensors; Skin; Skin - diagnostic imaging; Skin - metabolism; Spectrophotometry, Infrared - methods; Spectrum analysis

In vivo IR spectroscopy faces challenges due to poor sensitivity in reflection mode and low resolution at micrometer scale. To break this barrier, we report an oblique photothermal microscope (OPTM) to enable ultrasensitive IR spectroscopic imaging of live subjects at sub-micron resolution. Classic...