Tropomyosin variants describe distinct functional subcellular domains in differentiated vascular smooth muscle cells

Tropomyosin (Tm) is known to be an important gatekeeper of actin function. Tm isoforms are encoded by four genes, and each gene produces several variants by alternative splicing, which have been proposed to play roles in motility, proliferation, and apoptosis. Smooth muscle studies have focused on g...

Full description

Saved in:
Bibliographic Details
Published inAmerican Journal of Physiology: Cell Physiology Vol. 300; no. 6; pp. C1356 - C1365
Main Authors Gallant, Cynthia, Appel, Sarah, Graceffa, Philip, Leavis, Paul, Lin, Jim Jung-Ching, Gunning, Peter W, Schevzov, Galina, Chaponnier, Christine, DeGnore, Jon, Lehman, William, Morgan, Kathleen G
Format Journal Article
LanguageEnglish
Published United States American Physiological Society 01.06.2011
Subjects
Online AccessGet full text

Cover

Loading…
Abstract Tropomyosin (Tm) is known to be an important gatekeeper of actin function. Tm isoforms are encoded by four genes, and each gene produces several variants by alternative splicing, which have been proposed to play roles in motility, proliferation, and apoptosis. Smooth muscle studies have focused on gizzard smooth muscle, where a heterodimer of Tm from the α-gene (Tmsm-α) and from the β-gene (Tmsm-β) is associated with contractile filaments. In this study we examined Tm in differentiated mammalian vascular smooth muscle (dVSM). Liquid chromatography-tandem mass spectrometry (LC MS/MS) analysis and Western blot screening with variant-specific antibodies revealed that at least five different Tm proteins are expressed in this tissue: Tm6 (Tmsm-α) and Tm2 from the α-gene, Tm1 (Tmsm-β) from the β-gene, Tm5NM1 from the γ-gene, and Tm4 from the δ-gene. Tm6 is by far most abundant in dVSM followed by Tm1, Tm2, Tm5NM1, and Tm4. Coimmunoprecipitation and coimmunofluorescence studies demonstrate that Tm1 and Tm6 coassociate with different actin isoforms and display different intracellular localizations. Using an antibody specific for cytoplasmic γ-actin, we report here the presence of a γ-actin cortical cytoskeleton in dVSM cells. Tm1 colocalizes with cortical cytoplasmic γ-actin and coprecipitates with γ-actin. Tm6, on the other hand, is located on contractile bundles. These data indicate that Tm1 and Tm6 do not form a classical heterodimer in dVSM but rather describe different functional cellular compartments.
AbstractList Tropomyosin (Tm) is known to be an important gatekeeper of actin function. Tm isoforms are encoded by four genes, and each gene produces several variants by alternative splicing, which have been proposed to play roles in motility, proliferation, and apoptosis. Smooth muscle studies have focused on gizzard smooth muscle, where a heterodimer of Tm from the α-gene (Tmsm-α) and from the β-gene (Tmsm-β) is associated with contractile filaments. In this study we examined Tm in differentiated mammalian vascular smooth muscle (dVSM). Liquid chromatography-tandem mass spectrometry (LC MS/MS) analysis and Western blot screening with variant-specific antibodies revealed that at least five different Tm proteins are expressed in this tissue: Tm6 (Tmsm-α) and Tm2 from the α-gene, Tm1 (Tmsm-β) from the β-gene, Tm5NM1 from the γ-gene, and Tm4 from the δ-gene. Tm6 is by far most abundant in dVSM followed by Tm1, Tm2, Tm5NM1, and Tm4. Coimmunoprecipitation and coimmunofluorescence studies demonstrate that Tm1 and Tm6 coassociate with different actin isoforms and display different intracellular localizations. Using an antibody specific for cytoplasmic γ-actin, we report here the presence of a γ-actin cortical cytoskeleton in dVSM cells. Tm1 colocalizes with cortical cytoplasmic γ-actin and coprecipitates with γ-actin. Tm6, on the other hand, is located on contractile bundles. These data indicate that Tm1 and Tm6 do not form a classical heterodimer in dVSM but rather describe different functional cellular compartments.
Tropomyosin (Tm) is known to be an important gatekeeper of actin function. Tm isoforms are encoded by four genes, and each gene produces several variants by alternative splicing, which have been proposed to play roles in motility, proliferation, and apoptosis. Smooth muscle studies have focused on gizzard smooth muscle, where a heterodimer of Tm from the α-gene (Tmsm-α) and from the β-gene (Tmsm-β) is associated with contractile filaments. In this study we examined Tm in differentiated mammalian vascular smooth muscle (dVSM). Liquid chromatography-tandem mass spectrometry (LC MS/MS) analysis and Western blot screening with variant-specific antibodies revealed that at least five different Tm proteins are expressed in this tissue: Tm6 (Tmsm-α) and Tm2 from the α-gene, Tm1 (Tmsm-β) from the β-gene, Tm5NM1 from the ...-gene, and Tm4 from the ...-gene. Tm6 is by far most abundant in dVSM followed by Tm1, Tm2, Tm5NM1, and Tm4. Coimmunoprecipitation and coimmunofluorescence studies demonstrate that Tm1 and Tm6 coassociate with different actin isoforms and display different intracellular localizations. Using an antibody specific for cytoplasmic ...-actin, we report here the presence of a ...-actin cortical cytoskeleton in dVSM cells. Tm1 colocalizes with cortical cytoplasmic ...-actin and coprecipitates with ...-actin. Tm6, on the other hand, is located on contractile bundles. These data indicate that Tm1 and Tm6 do not form a classical heterodimer in dVSM but rather describe different functional cellular compartments. (ProQuest: ... denotes formulae/symbols omitted.)
Author Graceffa, Philip
Lin, Jim Jung-Ching
Gunning, Peter W
Lehman, William
DeGnore, Jon
Gallant, Cynthia
Leavis, Paul
Appel, Sarah
Schevzov, Galina
Chaponnier, Christine
Morgan, Kathleen G
Author_xml – sequence: 1
  givenname: Cynthia
  surname: Gallant
  fullname: Gallant, Cynthia
  organization: Health Sciences Dept., Boston University, 635 Commonwealth Ave., Boston, MA 02215, USA
– sequence: 2
  givenname: Sarah
  surname: Appel
  fullname: Appel, Sarah
– sequence: 3
  givenname: Philip
  surname: Graceffa
  fullname: Graceffa, Philip
– sequence: 4
  givenname: Paul
  surname: Leavis
  fullname: Leavis, Paul
– sequence: 5
  givenname: Jim Jung-Ching
  surname: Lin
  fullname: Lin, Jim Jung-Ching
– sequence: 6
  givenname: Peter W
  surname: Gunning
  fullname: Gunning, Peter W
– sequence: 7
  givenname: Galina
  surname: Schevzov
  fullname: Schevzov, Galina
– sequence: 8
  givenname: Christine
  surname: Chaponnier
  fullname: Chaponnier, Christine
– sequence: 9
  givenname: Jon
  surname: DeGnore
  fullname: DeGnore, Jon
– sequence: 10
  givenname: William
  surname: Lehman
  fullname: Lehman, William
– sequence: 11
  givenname: Kathleen G
  surname: Morgan
  fullname: Morgan, Kathleen G
BackLink https://www.ncbi.nlm.nih.gov/pubmed/21289288$$D View this record in MEDLINE/PubMed
BookMark eNpdkUtv1DAUhS1URKeFP8ACRWxYZfAjduwNEqp4SZXYlLXl-EE9SuzB16nUf4_TDhWwsRf3O8fn-lygs5STR-g1wXtCOH1vDkfr53mP8cDxnmKCn6FdG9CecMHO0A4zwXpBBnaOLgAOuIFUqBfonBIqFZVyh-pNyce83GeIqbszJZpUoXMebImT71yEGpOtXVjbGXMycwfrtD27zqZ0Li8mJuia2MUQfPGpRlO9a15gHxBYcq633bKCnX23KeEleh7MDP7V6b5EPz5_urn62l9___Lt6uN1bwc11D6MoxjIpLD31E7YcewDxZRSFyQ1CuPAOfF0JJJPalCSTkJa5YJXUhqiBLtEHx59j-u0eGdbuGJmfSxxMeVeZxP1v5MUb_XPfKcZIVIw0gzenQxK_rV6qHqJsK1gks8raClaCkz4Rr79jzzktbTvatBImeAjYQ2ij5AtGaD48BSFYL1Vqk-V6odK9VZpE735e4knyZ8O2W8LVaPg
CODEN AJPCDD
CitedBy_id crossref_primary_10_1152_ajpcell_00005_2013
crossref_primary_10_37349_emed_2021_00041
crossref_primary_10_1007_s10974_013_9355_8
crossref_primary_10_1007_s10974_013_9353_x
crossref_primary_10_1016_j_cophys_2019_09_006
crossref_primary_10_1002_ar_22983
crossref_primary_10_1091_mbc_E17_03_0152
crossref_primary_10_1113_JP270845
crossref_primary_10_1016_j_matbio_2019_03_001
crossref_primary_10_3390_biology11050662
crossref_primary_10_1074_jbc_M116_744011
crossref_primary_10_1002_cm_21230
crossref_primary_10_1161_CIRCRESAHA_116_309962
crossref_primary_10_1152_ajplung_00036_2014
crossref_primary_10_1152_ajpcell_00044_2021
crossref_primary_10_4161_bioa_1_4_17897
crossref_primary_10_1124_pr_115_010652
crossref_primary_10_1139_cjpp_2014_0413
crossref_primary_10_1113_jphysiol_2012_232306
crossref_primary_10_1113_jphysiol_2014_284174
crossref_primary_10_1007_s10974_012_9283_z
crossref_primary_10_1016_j_semcdb_2019_10_004
crossref_primary_10_1074_jbc_M114_553743
crossref_primary_10_1186_s13075_022_02730_z
crossref_primary_10_1074_jbc_M111_307330
crossref_primary_10_18632_oncotarget_16825
crossref_primary_10_1111_jcmm_16170
crossref_primary_10_1016_j_celrep_2022_111900
Cites_doi 10.1073/pnas.92.21.9776
10.1074/jbc.M410375200
10.1016/S0021-9258(19)67681-7
10.1089/hyb.1985.4.223
10.1006/mcne.1997.0599
10.1016/0092-8674(89)90678-8
10.1152/ajpcell.00431.2009
10.1083/jcb.106.2.385
10.1016/j.jsb.2009.12.016
10.1021/bi00237a007
10.1083/jcb.129.3.697
10.1016/S1937-6448(10)81003-2
10.1091/mbc.E03-03-0169
10.1083/jcb.107.2.563
10.1007/s11373-005-9060-8
10.1016/S0006-291X(86)80328-X
10.1083/jcb.121.4.811
10.1007/s10974-008-9157-6
10.1073/pnas.121146098
10.1016/S0021-9258(19)69568-2
10.1098/rspb.1980.0024
10.1007/978-0-387-85766-4_9
10.1369/jhc.4A6505.2005
10.1083/jcb.100.3.692
10.1007/978-0-387-85766-4_15
10.1007/978-4-431-38453-3_9
10.1006/mcne.1998.0693
10.1002/cm.970250307
10.1006/abbi.2000.1802
10.1152/physrev.00001.2007
10.1016/S0021-9258(18)90828-8
10.1242/jcs.041970
10.1016/S0021-9258(17)39602-3
10.1128/MCB.8.2.679
10.1111/j.1582-4934.2008.00552.x
10.1016/S0021-9258(17)42610-X
10.1128/MCB.10.4.1729
10.1016/j.tcb.2005.04.007
10.1016/B978-012078160-7/50007-X
10.1073/pnas.76.2.819
10.1021/ac00104a020
10.3109/10409238409108717
10.1021/bi00299a029
10.1152/ajpcell.00174.2008
10.1242/jcs.107.3.445
10.1146/annurev.bb.16.060187.002535
10.1016/S0016-5085(98)70310-5
10.1007/978-0-387-85766-4_3
10.1016/S0074-7696(08)61619-8
10.1165/ajrcmb.15.1.8679216
10.1016/B978-0-12-152827-0.50012-8
10.1111/j.1469-7793.1998.187br.x
10.1007/s003350010172
10.1007/978-0-387-85766-4_16
10.1007/978-0-387-85766-4_2
10.1083/jcb.109.3.1141
10.1002/cm.970260308
10.1093/oxfordjournals.jbchem.a123473
10.1016/S0021-9258(18)67160-1
ContentType Journal Article
Copyright Copyright American Physiological Society Jun 2011
Copyright © 2011 the American Physiological Society 2011
Copyright_xml – notice: Copyright American Physiological Society Jun 2011
– notice: Copyright © 2011 the American Physiological Society 2011
DBID CGR
CUY
CVF
ECM
EIF
NPM
AAYXX
CITATION
7QP
7TS
7X8
5PM
DOI 10.1152/ajpcell.00450.2010
DatabaseName Medline
MEDLINE
MEDLINE (Ovid)
MEDLINE
MEDLINE
PubMed
CrossRef
Calcium & Calcified Tissue Abstracts
Physical Education Index
MEDLINE - Academic
PubMed Central (Full Participant titles)
DatabaseTitle MEDLINE
Medline Complete
MEDLINE with Full Text
PubMed
MEDLINE (Ovid)
CrossRef
Calcium & Calcified Tissue Abstracts
Physical Education Index
MEDLINE - Academic
DatabaseTitleList MEDLINE
Calcium & Calcified Tissue Abstracts
CrossRef

Database_xml – sequence: 1
  dbid: NPM
  name: PubMed
  url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
– sequence: 2
  dbid: EIF
  name: MEDLINE
  url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search
  sourceTypes: Index Database
DeliveryMethod fulltext_linktorsrc
Discipline Anatomy & Physiology
Biology
EISSN 1522-1563
EndPage C1365
ExternalDocumentID 2377790981
10_1152_ajpcell_00450_2010
21289288
Genre Research Support, Non-U.S. Gov't
Journal Article
Research Support, N.I.H., Extramural
GrantInformation_xml – fundername: NHLBI NIH HHS
  grantid: HL-31704
– fundername: NHLBI NIH HHS
  grantid: HL-80003
– fundername: NHLBI NIH HHS
  grantid: HL-86655
GroupedDBID ---
23M
2WC
39C
4.4
53G
5GY
5VS
6J9
85S
8M5
AAFWJ
ABJNI
ACGFS
ACPRK
ADBBV
AENEX
AFFNX
AIZTS
ALMA_UNASSIGNED_HOLDINGS
BAWUL
BKKCC
BKOMP
BTFSW
C1A
CGR
CUY
CVF
DIK
E3Z
EBS
ECM
EIF
EJD
EMOBN
F5P
GX1
H13
ITBOX
KQ8
NPM
OK1
P2P
PQQKQ
RAP
RHF
RHI
RPL
RPRKH
TR2
W8F
WH7
WOQ
XSW
YSK
~02
AAYXX
CITATION
7QP
7TS
7X8
5PM
ID FETCH-LOGICAL-c494t-f77641b90ee2cb0d50ef20222df82a900f551e27185b94982b68c9dfe988a1963
ISSN 0363-6143
IngestDate Tue Sep 17 21:24:07 EDT 2024
Wed Dec 04 14:40:27 EST 2024
Thu Oct 10 18:22:35 EDT 2024
Fri Dec 06 05:38:25 EST 2024
Sat Sep 28 07:50:21 EDT 2024
IsDoiOpenAccess false
IsOpenAccess true
IsPeerReviewed true
IsScholarly true
Issue 6
Language English
LinkModel OpenURL
MergedId FETCHMERGED-LOGICAL-c494t-f77641b90ee2cb0d50ef20222df82a900f551e27185b94982b68c9dfe988a1963
Notes ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
C. Gallant and S. Appel contributed equally to this work.
OpenAccessLink https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3118631
PMID 21289288
PQID 872365713
PQPubID 48267
ParticipantIDs pubmedcentral_primary_oai_pubmedcentral_nih_gov_3118631
proquest_miscellaneous_869000151
proquest_journals_872365713
crossref_primary_10_1152_ajpcell_00450_2010
pubmed_primary_21289288
PublicationCentury 2000
PublicationDate 2011-06-01
PublicationDateYYYYMMDD 2011-06-01
PublicationDate_xml – month: 06
  year: 2011
  text: 2011-06-01
  day: 01
PublicationDecade 2010
PublicationPlace United States
PublicationPlace_xml – name: United States
– name: Bethesda
– name: Bethesda, MD
PublicationTitle American Journal of Physiology: Cell Physiology
PublicationTitleAlternate Am J Physiol Cell Physiol
PublicationYear 2011
Publisher American Physiological Society
Publisher_xml – name: American Physiological Society
References 3899907 - Hybridoma. 1985 Fall;4(3):223-42
3352602 - Mol Cell Biol. 1988 Feb;8(2):679-94
3047141 - J Cell Biol. 1988 Aug;107(2):563-72
6231950 - Biochemistry. 1984 Feb 14;23(4):774-9
2320008 - Mol Cell Biol. 1990 Apr;10(4):1729-42
3745212 - J Biol Chem. 1986 Sep 25;261(27):12774-8
19116763 - J Muscle Res Cell Motil. 2008;29(6-8):213-9
15953552 - Trends Cell Biol. 2005 Jun;15(6):333-41
18596213 - Am J Physiol Cell Physiol. 2008 Sep;295(3):C768-78
19209824 - Adv Exp Med Biol. 2008;644:201-22
3606745 - Biochem Biophys Res Commun. 1986 Nov 26;141(1):20-6
11416185 - Proc Natl Acad Sci U S A. 2001 Jun 19;98(13):7045-50
9698395 - Mol Cell Neurosci. 1998 Aug;11(5-6):289-304
15872049 - J Histochem Cytochem. 2005 May;53(5):557-70
8006065 - J Cell Sci. 1994 Mar;107 ( Pt 3):445-55
7730405 - J Cell Biol. 1995 May;129(3):697-708
3997867 - J Biol Chem. 1985 Jun 25;260(12):7264-75
7568216 - Proc Natl Acad Sci U S A. 1995 Oct 10;92(21):9776-80
9558279 - Gastroenterology. 1998 May;114(5):912-22
2982883 - J Cell Biol. 1985 Mar;100(3):692-703
19209817 - Adv Exp Med Biol. 2008;644:110-23
12960432 - Mol Biol Cell. 2003 Nov;14(11):4365-75
1939008 - J Biochem. 1991 Jun;109(6):872-8
11003701 - Mamm Genome. 2000 Oct;11(10):866-70
6383715 - CRC Crit Rev Biochem. 1984;16(3):235-305
2954560 - Annu Rev Biophys Biophys Chem. 1987;16:535-59
15504719 - J Biol Chem. 2005 Feb 11;280(6):4135-43
19638415 - J Cell Sci. 2009 Aug 15;122(Pt 16):2980-8
9490838 - J Physiol. 1998 Apr 1;508 ( Pt 1):187-98
2936578 - Curr Top Cell Regul. 1985;27:51-62
19120701 - J Cell Mol Med. 2008 Dec;12(6A):2165-80
19209823 - Adv Exp Med Biol. 2008;644:187-200
2448313 - J Cell Biol. 1988 Feb;106(2):385-93
8679216 - Am J Respir Cell Mol Biol. 1996 Jul;15(1):122-31
20018948 - Am J Physiol Cell Physiol. 2010 Mar;298(3):C559-71
2043610 - Biochemistry. 1991 Jun 11;30(23):5682-8
2670955 - J Cell Biol. 1989 Sep;109(3):1141-52
8293480 - Cell Motil Cytoskeleton. 1993;26(3):248-61
16453176 - J Biomed Sci. 2006 Mar;13(2):159-72
17278358 - Adv Exp Med Biol. 2007;592:87-97
9002235 - Int Rev Cytol. 1997;170:1-38
10871039 - Arch Biochem Biophys. 2000 Jun 1;378(1):16-24
7916663 - Cell Motil Cytoskeleton. 1993;25(3):267-81
19209810 - Adv Exp Med Biol. 2008;644:6-26
9143561 - Mol Cell Neurosci. 1997;8(6):439-54
20026406 - J Struct Biol. 2010 May;170(2):286-93
6102396 - Proc R Soc Lond B Biol Sci. 1980 Mar 21;207(1168):259-86
18195081 - Physiol Rev. 2008 Jan;88(1):1-35
2924347 - Cell. 1989 Mar 10;56(5):749-58
8491774 - J Cell Biol. 1993 May;121(4):811-21
7741214 - Anal Chem. 1995 Apr 15;67(8):1426-36
284403 - Proc Natl Acad Sci U S A. 1979 Feb;76(2):819-23
20460184 - Int Rev Cell Mol Biol. 2010;281:91-128
7217070 - J Biol Chem. 1981 Apr 25;256(8):4081-6
2005125 - J Biol Chem. 1991 Mar 25;266(9):5891-7
6501298 - J Biol Chem. 1984 Dec 10;259(23):14383-8
6092349 - J Biol Chem. 1984 Oct 25;259(20):12873-80
4021438 - Lab Anim Sci. 1985 Jun;35(3):246-50
19209811 - Adv Exp Med Biol. 2008;644:27-42
B20
B21
B22
B23
B24
B25
B26
B27
B28
North AJ (B37) 1994; 107
B29
Hartshorne DJ (B14) 1987
Vinegar A (B54) 1985; 35
B30
B31
B32
B33
B34
B35
Bretscher A (B1) 1984; 259
B38
B39
B2
B3
B4
B5
B7
B8
B9
B40
B41
B45
B46
B47
B48
B49
Sanders C (B44) 1985; 260
B50
B51
B52
B53
B10
B11
B55
B12
B56
B13
B57
B58
B15
Jancso A (B19) 1991; 266
B59
B16
B17
B18
Fatigati V (B6) 1984; 259
Montarras D (B36) 1981; 256
Sanders C (B43) 1986; 261
Ross R (B42) 1980
B60
B61
B62
References_xml – ident: B8
  doi: 10.1073/pnas.92.21.9776
– ident: B10
  doi: 10.1074/jbc.M410375200
– volume: 266
  start-page: 5891
  year: 1991
  ident: B19
  publication-title: J Biol Chem
  doi: 10.1016/S0021-9258(19)67681-7
  contributor:
    fullname: Jancso A
– ident: B28
  doi: 10.1089/hyb.1985.4.223
– start-page: 69
  volume-title: Handbook of Physiology. The Cardiovascular System. Vascular Smooth Muscle
  year: 1980
  ident: B42
  contributor:
    fullname: Ross R
– ident: B45
  doi: 10.1006/mcne.1997.0599
– ident: B51
  doi: 10.1016/0092-8674(89)90678-8
– ident: B22
  doi: 10.1152/ajpcell.00431.2009
– ident: B15
  doi: 10.1083/jcb.106.2.385
– ident: B35
  doi: 10.1016/j.jsb.2009.12.016
– ident: B27
  doi: 10.1021/bi00237a007
– ident: B57
  doi: 10.1083/jcb.129.3.697
– ident: B56
  doi: 10.1016/S1937-6448(10)81003-2
– ident: B3
  doi: 10.1091/mbc.E03-03-0169
– start-page: 423
  volume-title: Physiology of the Gastrointestinal Tract
  year: 1987
  ident: B14
  contributor:
    fullname: Hartshorne DJ
– ident: B30
  doi: 10.1083/jcb.107.2.563
– ident: B23
  doi: 10.1007/s11373-005-9060-8
– ident: B52
  doi: 10.1016/S0006-291X(86)80328-X
– ident: B46
  doi: 10.1083/jcb.121.4.811
– ident: B18
  doi: 10.1007/s10974-008-9157-6
– ident: B50
  doi: 10.1073/pnas.121146098
– volume: 256
  start-page: 4081
  year: 1981
  ident: B36
  publication-title: J Biol Chem
  doi: 10.1016/S0021-9258(19)69568-2
  contributor:
    fullname: Montarras D
– ident: B5
  doi: 10.1098/rspb.1980.0024
– ident: B33
  doi: 10.1007/978-0-387-85766-4_9
– ident: B47
  doi: 10.1369/jhc.4A6505.2005
– ident: B31
  doi: 10.1083/jcb.100.3.692
– ident: B34
  doi: 10.1007/978-0-387-85766-4_15
– ident: B17
  doi: 10.1007/978-4-431-38453-3_9
– ident: B13
  doi: 10.1006/mcne.1998.0693
– ident: B38
  doi: 10.1002/cm.970250307
– ident: B53
  doi: 10.1006/abbi.2000.1802
– ident: B11
  doi: 10.1152/physrev.00001.2007
– volume: 259
  start-page: 12873
  year: 1984
  ident: B1
  publication-title: J Biol Chem
  doi: 10.1016/S0021-9258(18)90828-8
  contributor:
    fullname: Bretscher A
– ident: B4
  doi: 10.1242/jcs.041970
– volume: 260
  start-page: 7264
  year: 1985
  ident: B44
  publication-title: J Biol Chem
  doi: 10.1016/S0021-9258(17)39602-3
  contributor:
    fullname: Sanders C
– ident: B58
  doi: 10.1128/MCB.8.2.679
– ident: B20
  doi: 10.1111/j.1582-4934.2008.00552.x
– volume: 259
  start-page: 14383
  year: 1984
  ident: B6
  publication-title: J Biol Chem
  doi: 10.1016/S0021-9258(17)42610-X
  contributor:
    fullname: Fatigati V
– ident: B25
  doi: 10.1128/MCB.10.4.1729
– ident: B12
  doi: 10.1016/j.tcb.2005.04.007
– ident: B26
  doi: 10.1016/B978-012078160-7/50007-X
– ident: B40
  doi: 10.1073/pnas.76.2.819
– ident: B61
  doi: 10.1021/ac00104a020
– ident: B24
  doi: 10.3109/10409238409108717
– ident: B60
  doi: 10.1021/bi00299a029
– ident: B21
  doi: 10.1152/ajpcell.00174.2008
– volume: 107
  start-page: 445
  year: 1994
  ident: B37
  publication-title: J Cell Sci
  doi: 10.1242/jcs.107.3.445
  contributor:
    fullname: North AJ
– ident: B62
  doi: 10.1146/annurev.bb.16.060187.002535
– volume: 35
  start-page: 246
  year: 1985
  ident: B54
  publication-title: Lab Anim Sci
  contributor:
    fullname: Vinegar A
– ident: B7
  doi: 10.1016/S0016-5085(98)70310-5
– ident: B9
  doi: 10.1007/978-0-387-85766-4_3
– ident: B32
  doi: 10.1016/S0074-7696(08)61619-8
– ident: B48
  doi: 10.1165/ajrcmb.15.1.8679216
– ident: B49
  doi: 10.1016/B978-0-12-152827-0.50012-8
– ident: B41
  doi: 10.1111/j.1469-7793.1998.187br.x
– ident: B2
  doi: 10.1007/s003350010172
– ident: B29
  doi: 10.1007/978-0-387-85766-4_16
– ident: B55
  doi: 10.1007/978-0-387-85766-4_2
– ident: B16
  doi: 10.1083/jcb.109.3.1141
– ident: B39
  doi: 10.1002/cm.970260308
– ident: B59
  doi: 10.1093/oxfordjournals.jbchem.a123473
– volume: 261
  start-page: 12774
  year: 1986
  ident: B43
  publication-title: J Biol Chem
  doi: 10.1016/S0021-9258(18)67160-1
  contributor:
    fullname: Sanders C
SSID ssj0004269
Score 2.2367125
Snippet Tropomyosin (Tm) is known to be an important gatekeeper of actin function. Tm isoforms are encoded by four genes, and each gene produces several variants by...
SourceID pubmedcentral
proquest
crossref
pubmed
SourceType Open Access Repository
Aggregation Database
Index Database
StartPage C1356
SubjectTerms Actins - genetics
Actins - metabolism
Amino Acid Sequence
Animals
Cell Differentiation - physiology
Chickens
Chromatography
Cytoskeleton
Ferrets
Genes
Humans
Mass spectrometry
Molecular Sequence Data
Muscle Cell Biology and Cell Motility
Muscle, Smooth, Vascular - cytology
Myocytes, Smooth Muscle - cytology
Myocytes, Smooth Muscle - physiology
Protein Binding
Protein Isoforms - chemistry
Protein Isoforms - genetics
Protein Isoforms - metabolism
Proteins
Sequence Alignment
Studies
Tropomyosin - chemistry
Tropomyosin - genetics
Tropomyosin - metabolism
Title Tropomyosin variants describe distinct functional subcellular domains in differentiated vascular smooth muscle cells
URI https://www.ncbi.nlm.nih.gov/pubmed/21289288
https://www.proquest.com/docview/872365713
https://search.proquest.com/docview/869000151
https://pubmed.ncbi.nlm.nih.gov/PMC3118631
Volume 300
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Nj9MwELXKIiQuCHb5KAvIB8QlSklsx4mPVQWs-JKQdqW9RXbiaLtS02qbIpV_wr9lbMepuwUJuERVnMRp3st4ZjJ-Rug1NxpWqaximXIVM9LIuGC0iYnkijJRFEKbucNfvvKzC_bxMrscjX4GVUubTk2qH7-dV_I_qMI-wNXMkv0HZIeLwg74DfjCFhCG7d9hbJY4WGyXEO9H3yHotTUttTaWQGnz7aWbt1UXmbGrT_mtN8qk6m3tab1cyLmrI_fLpMDrbjzQoTx1vVgCktFis4aeI3PmOvRmh889gVtrS0rdFBg6jWY2NTjsGqp9TPreKR7Mtm13NR8GB_CKXd3AXqr6w42sdNPIXQrIt3zWXiZhqHGsd0lZX2s10b3dhZgYQkkaGmaaJAEDQzM7S6mTIz8cADIjKCuvV-aRTIzDmtj6vfBg-PurhaUEjNuFIG5dwVuy277pDrprNBbNsgyfvgVC9IQLPwcrI28PO7Qq0-4S-y7PQRxzuxw38G_OH6IHPYJ46lj2CI10e4xOpq3sgGH4Dd6BeIzuuRVMtyeoCyiIPQWxpyD2FMQ7CuKAgrinIIaT9ymIPQWxoyB2FMSWgo_Rxft357OzuF_II66YYF3c5DlnqRKJ1qRSSZ0luiEm01A3BZEiSRrw2zUBNylTAgwEUbyoRN1osBXSDBFP0FG7bPUzhBVnumEJJTmTrFZKFJTpShZEwL2zpBijyD_rcuX0Wkob52ak7EEqLUilAWmMTj0cZf9er8siJ5RneUrHCA-tYHTNubLVyw0cwoUVIUjH6KnDbujMgz5G-R6qwwFGz32_pZ1fWV13CsE-p-nzP17zFN3fvT0v0FF3s9EvwSfu1CvLzl-E1sC5
link.rule.ids 230,314,780,784,885,27924,27925
linkProvider Colorado Alliance of Research Libraries
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Tropomyosin+variants+describe+distinct+functional+subcellular+domains+in+differentiated+vascular+smooth+muscle+cells&rft.jtitle=American+Journal+of+Physiology%3A+Cell+Physiology&rft.au=Gallant%2C+Cynthia&rft.au=Appel%2C+Sarah&rft.au=Graceffa%2C+Philip&rft.au=Leavis%2C+Paul&rft.date=2011-06-01&rft.eissn=1522-1563&rft.volume=300&rft.issue=6&rft.spage=C1356&rft_id=info:doi/10.1152%2Fajpcell.00450.2010&rft_id=info%3Apmid%2F21289288&rft.externalDocID=21289288
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0363-6143&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0363-6143&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0363-6143&client=summon