Decreased expression of insulin-degrading enzyme increases gluconeogenesis and glucose production in cultured hepatocytes administered with glucagon

• Published in: Scientific reports Vol. 15; no. 1; pp. 19168 - 12
• Main Authors: González-Casimiro, Carlos M., Cámara-Torres, Patricia, Merino, Beatriz, Astudillo, Alma M., de la Fuente, Miguel A., Ramírez, Cristina M., Alonso, Andrés, Cózar-Castellano, Irene, Perdomo, Germán
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 31.05.2025; Nature Publishing Group; Nature Portfolio
• Subjects: 631/45/776; 631/80/86; Animal tissues; Animals; Blood glucose; Cells, Cultured; Cyclic AMP response element-binding protein; Cyclic AMP Response Element-Binding Protein - metabolism; Diabetes mellitus (non-insulin dependent); Diabetes Mellitus, Type 2 - metabolism; Enzymes; Glucagon; Glucagon - administration & dosage; Glucagon - metabolism; Glucagon - pharmacology; Gluconeogenesis; Gluconeogenesis - drug effects; Gluconeogenesis - genetics; Glucose; Glucose - biosynthesis; Glucose - metabolism; Hepatocytes; Hepatocytes - drug effects; Hepatocytes - metabolism; Homeostasis; Humanities and Social Sciences; Humans; Hypothesis testing; Insulin; Insulin-degrading enzyme; Insulysin; Insulysin - genetics; Insulysin - metabolism; Liver; Liver - metabolism; Male; Mice; Mice, Knockout; multidisciplinary; Organelles; Phosphorylation; Proteolysis; Receptors, Glucagon - metabolism; Science; Science (multidisciplinary); Signal Transduction; Therapeutic targets; Transcriptomics; Up-regulation; Glucagon; Hepatocytes; Insulin-degrading enzyme; Gluconeogenesis

Insulin-degrading enzyme (IDE) is a protein with proteolytic and non-proteolytic functions that regulates glucose homeostasis. In the fasted state, glucagon regulates glycemia through induction of hepatic gluconeogenesis. The rate of hepatic gluconeogenesis is elevated in subjects with type 2 diabet...