The effects of inhibiting IRE1α on the viability of ovarian granulosa cells

• Published in: Scientific reports Vol. 15; no. 1; pp. 19512 - 10
• Main Authors: Li, Chao, Tan, Yong-Peng, Meng, Tie-Gang, Gao, Di, Xu, Ke, Lu, You-Hui, Yi, Li-Tao, Liu, Shu-Chen, Wang, Guang, Sun, Qing-Yuan, Lei, Xiao-Can
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 04.06.2025; Nature Publishing Group; Nature Portfolio
• Subjects: 4µ8c; 631/136; 631/80; 631/92; Apoptosis; Apoptosis - drug effects; APY29; Cell apoptosis; Cell cycle; Cell proliferation; Cell Proliferation - drug effects; Cell Survival - drug effects; Cells, Cultured; Chaperones; DNA damage; DNA Damage - drug effects; Drug dosages; Endoplasmic Reticulum Stress - drug effects; Endoribonucleases - antagonists & inhibitors; Endoribonucleases - metabolism; Female; Females; Fertility; Flow cytometry; Granulosa cells; Granulosa Cells - cytology; Granulosa Cells - drug effects; Granulosa Cells - metabolism; Humanities and Social Sciences; Humans; IRE1α; Kinases; Medicine; Membrane potential; Membrane Potential, Mitochondrial - drug effects; multidisciplinary; Ovarian granulosa cells; Ovaries; Oxidative stress; Oxidative Stress - drug effects; Phosphorylation - drug effects; Physiology; Protein folding; Protein Serine-Threonine Kinases - antagonists & inhibitors; Protein Serine-Threonine Kinases - metabolism; Protein-serine/threonine kinase; Proteins; Reproductive health; Science; Science (multidisciplinary); APY29; Ovarian granulosa cells; Chaperones; 4µ8c; IRE1α; Cell apoptosis

IRE1α, a type I transmembrane protein characterized by a cytoplasmic serine/threonine kinase domain, is related to ER stress and ER function maintenance. In this study, 4µ8c, a highly effective selective inhibitor of IRE1α RNase, and APY29, an ATP competitive inhibitor, inhibiting IRE1α autophosphor...