Discovery of a Novel Mycobacterium tuberculosis Lineage That Is a Major Cause of Tuberculosis in Rio de Janeiro, Brazil

• Published in: Journal of Clinical Microbiology Vol. 45; no. 12; pp. 3891 - 3902
• Main Authors: Lazzarini, Luiz Claudio Oliveira, Huard, Richard C, Boechat, Neio L, Gomes, Harrison M, Oelemann, Maranibia C, Kurepina, Natalia, Shashkina, Elena, Mello, Fernanda C.Q, Gibson, Andrea L, Virginio, Milena J, Marsico, Ana Grazia, Butler, W. Ray, Kreiswirth, Barry N, Suffys, Philip N, Lapa e Silva, Jose Roberto, Ho, John L
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.12.2007; American Society for Microbiology (ASM)
• Subjects: Animals; Bacterial diseases; Bacteriology; Biological and medical sciences; Brazil - epidemiology; Cluster Analysis; DNA Fingerprinting; DNA Transposable Elements - genetics; DNA, Bacterial - genetics; Fundamental and applied biological sciences. Psychology; Genotype; Human bacterial diseases; Humans; Infectious diseases; Medical sciences; Microbiology; Minisatellite Repeats - genetics; Miscellaneous; Mycobacteriology and Aerobic Actinomycetes; Mycobacterium; Mycobacterium tuberculosis; Mycobacterium tuberculosis - classification; Mycobacterium tuberculosis - genetics; Mycobacterium tuberculosis - isolation & purification; Mycobacterium tuberculosis - pathogenicity; Phylogeny; Polymerase Chain Reaction - methods; Polymorphism, Genetic; Polymorphism, Restriction Fragment Length; Recombination, Genetic; Sequence Deletion; Tuberculosis - epidemiology; Tuberculosis - microbiology; Tuberculosis - pathology; Tuberculosis - physiopathology; Tuberculosis and atypical mycobacterial infections; South America; Brazil; America; Infection; Tuberculosis; Mycobacterium tuberculosis; Mycobacteriales; Bacteriosis; Mycobacteriaceae; Bacteria; Actinomycetes; Mycobacterial infection

The current study evaluated Mycobacterium tuberculosis isolates from Rio de Janeiro, Brazil, for genomic deletions. One locus in our panel of PCR targets failed to amplify in ~30% of strains. A single novel long sequence polymorphism (>26.3 kb) was characterized and designated RDRio. Homologous r...