Mutations at the Base of the Icosahedral Five-Fold Cylinders of Minute Virus of Mice Induce 3'-to-5' Genome Uncoating and Critically Impair Entry Functions

• Published in: Journal of Virology Vol. 86; no. 1; pp. 69 - 80
• Main Authors: Cotmore, Susan F, Tattersall, Peter
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 2012
• Subjects: Animals; Biological and medical sciences; Cell Line; coat proteins; DNA, Single-Stranded - chemistry; DNA, Single-Stranded - genetics; DNA, Viral - chemistry; DNA, Viral - genetics; Fundamental and applied biological sciences. Psychology; genome; Genome and Regulation of Viral Gene Expression; Genome, Viral; Mice; Microbiology; Minute virus of mice; Minute Virus of Mice - chemistry; Minute Virus of Mice - genetics; Minute Virus of Mice - physiology; Miscellaneous; mutants; Mutation; Nucleic Acid Conformation; Parvoviridae Infections - veterinary; Parvoviridae Infections - virology; phenotype; progeny; proteolysis; Rodent Diseases - virology; Rodent protoparvovirus 1; single-stranded DNA; virion; Virology; Virus Assembly; Virus Internalization; Virus Replication; viruses; Virus; Parvovirus; Parvoviridae; Mutation; Genome; Parvovirinae; Mouse minute virus

The linear single-stranded DNA genome of minute virus of mice can be ejected, in a 3'-to-5' direction, via a cation-linked uncoating reaction that leaves the 5' end of the DNA firmly complexed with its otherwise intact protein capsid. Here we compare the phenotypes of four mutants, L1...