Cloning, Expression, and Characterization of a Novel Thermophilic Monofunctional Catalase from Geobacillus sp. CHB1

• Published in: BioMed research international Vol. 2016; no. 2016; pp. 1 - 8
• Main Authors: Lin, Xinjian, Lin, Chenqiang, Chen, Jichen, Jia, Xianbo
• Format: Journal Article
• Language: English
• Published: Cairo, Egypt Hindawi Publishing Corporation 01.01.2016; John Wiley & Sons, Inc; Hindawi Limited
• Subjects: Amino acids; Bacillus; Bacteria; Bacterial Proteins - biosynthesis; Bacterial Proteins - chemistry; Bacterial Proteins - genetics; Bacterial Proteins - isolation & purification; Catalase - biosynthesis; Catalase - chemistry; Catalase - genetics; Catalase - isolation & purification; Cloning; Cloning, Molecular; E coli; Enzyme kinetics; Enzymes; Escherichia coli; Fermentation; Gene Expression; Genetic engineering; Geobacillus; Geobacillus - enzymology; Geobacillus - genetics; Phylogenetics; Recombinant Proteins - biosynthesis; Recombinant Proteins - chemistry; Recombinant Proteins - genetics; Recombinant Proteins - isolation & purification; China

Catalases are widely used in many scientific areas. A catalase gene (Kat) from Geobacillus sp. CHB1 encoding a monofunctional catalase was cloned and recombinant expressed in Escherichia coli (E. coli), which was the first time to clone and express this type of catalase of genus Geobacillus strains...