Transcriptome-Wide Mapping of 5-methylcytidine RNA Modifications in Bacteria, Archaea, and Yeast Reveals m5C within Archaeal mRNAs

• Published in: PLoS genetics Vol. 9; no. 6; p. e1003602
• Main Authors: Edelheit, Sarit, Schwartz, Schraga, Mumbach, Maxwell R., Wurtzel, Omri, Sorek, Rotem
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 01.06.2013; Public Library of Science (PLoS)
• Subjects: Archaea - genetics; Bacillus subtilis - genetics; Bacteria; Biology; Cytidine - analogs & derivatives; Cytidine - genetics; DNA methylation; E coli; Escherichia coli - genetics; Eukaryotes; Gene Expression Profiling; Genes; Genetics; Genome; Genomes; High-Throughput Nucleotide Sequencing; Methylation; Organisms; Proteins; RNA, Messenger - genetics; RNA, Ribosomal - genetics; RNA, Transfer - genetics; Saccharomyces cerevisiae - genetics; Studies; Transfer RNA; Yeast

The presence of 5-methylcytidine (m(5)C) in tRNA and rRNA molecules of a wide variety of organisms was first observed more than 40 years ago. However, detection of this modification was limited to specific, abundant, RNA species, due to the usage of low-throughput methods. To obtain a high resolutio...